Genetic diversity and population structure of the primary malaria vector Anopheles sinensis (Diptera: Culicidae) in China inferred by cox1 gene

Feng, Xinyu; Huang, Libin; Lin, Lin; Mu, Yang; Ma, Yajun

doi:10.1186/s13071-017-2013-z

Cited by 15 publications

(22 citation statements)

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“…L20934.1) and other Anopheles mosquitoes. Some parts fragments such as CO1 and CO2 region were verified by PCR products [ 7 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…The genetic structure of An. sinensis populations in China were also detected by molecular markers, and the weak genetic structure may be a consequence of low genetic differentiation and high gene flow among populations in central China [ 7 – 10 ]. However, there are still some issues to be elucidated in the molecular classification of An.…”

Section: Introductionmentioning

confidence: 99%

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Sequencing and analysis of the complete mitochondrial genome in Anopheles sinensis (Diptera: Culicidae)

Chen

Wang

et al. 2017

Infect Dis Poverty

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Background Anopheles sinensis (Diptera: Culicidae) is a primary vector of Plasmodium vivax and Brugia malayi in most regions of China. In addition, its phylogenetic relationship with the cryptic species of the Hyrcanus Group is complex and remains unresolved. Mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, of which mitochondrial genome data of An. sinensis is not available.Methods An. sinensis samples was collected from Shandong, China, and identified by molecular marker. Genomic DNA was extracted, followed by the Illumina sequencing. Two complete mitochondrial genomes were assembled and annotated using the mitochondrial genome of An. gambiae as reference. The mitochondrial genomes sequences of the 28 known Anopheles species were aligned and reconstructed phylogenetic tree by Maximum Likelihood (ML) method.FindingsThe length of complete mitochondrial genomes of An. sinensis was 15,076 bp and 15,138 bp, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and an AT-rich control region. As in other insects, most mitochondrial genes are encoded on the J strand, except for ND5, ND4, ND4L, ND1, two rRNA and eight tRNA genes, which are encoded on the N strand. The bootstrap value was set as 1000 in ML analyses. The topologies restored phylogenetic affinity within subfamily Anophelinae. The ML tree showed four major clades, corresponding to the subgenera Cellia, Anopheles, Nyssorhynchus and Kerteszia of the genus Anopheles.ConclusionsThe complete mitochondrial genomes of An. sinensis were obtained. The number, order and transcription direction of An. sinensis mitochondrial genes were the same as in other species of family Culicidae.Electronic supplementary materialThe online version of this article (10.1186/s40249-017-0362-7) contains supplementary material, which is available to authorized users.

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“…L20934.1) and other Anopheles mosquitoes. Some parts fragments such as CO1 and CO2 region were verified by PCR products [ 7 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequencing and analysis of the complete mitochondrial genome in Anopheles sinensis (Diptera: Culicidae)

Chen

Wang

et al. 2017

Infect Dis Poverty

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“…Different An. sinensis populations in China exhibit variability in morphology, chromosomes, ecology, vector capacity, mitochondrial DNA, and even entire genomic DNA composition ( Feng et al, 2017 ). Understanding the molecular and genetic mechanisms that determine variability in transmission efficiency and mosquito susceptibility could aid in the development of novel vector control strategies.…”

Section: Discussionmentioning

confidence: 99%

Biology, Bionomics and Molecular Biology of Anopheles sinensis Wiedemann 1828 (Diptera: Culicidae), Main Malaria Vector in China

et al. 2017

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China has set a goal to eliminate all malaria in the country by 2020, but it is unclear if current understanding of malaria vectors and transmission is sufficient to achieve this objective. Anopheles sinensis is the most widespread malaria vector specie in China, which is also responsible for vivax malaria outbreak in central China. We reviewed literature from 1954 to 2016 on An. sinensis with emphasis on biology, bionomics, and molecular biology. A total of 538 references were relevant and included. An. sienesis occurs in 29 Chinese provinces. Temperature can affect most life-history parameters. Most An. sinensis are zoophilic, but sometimes they are facultatively anthropophilic. Sporozoite analysis demonstrated An. sinensis efficacy on Plasmodium vivax transmission. An. sinensis was not stringently refractory to P. falciparum under experimental conditions, however, sporozoite was not found in salivary glands of field collected An. sinensis. The literature on An. sienesis biology and bionomics was abundant, but molecular studies, such as gene functions and mechanisms, were limited. Only 12 molecules (genes, proteins or enzymes) have been studied. In addition, there were considerable untapped omics resources for potential vector control tools. Existing information on An. sienesis could serve as a baseline for advanced research on biology, bionomics and genetics relevant to vector control strategies.

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“…Anopheles sinensis is one of the most important disease vectors in China. It is considered as the primary vector of P. vivax malaria due to its wide distribution and high density in central China where several malaria outbreaks occurred in history [ 1 , 2 ]. Like many mosquitoes, An.…”

Section: Introductionmentioning

confidence: 99%

Characterization and potential role of microRNA in the Chinese dominant malaria mosquito Anopheles sinensis (Diptera: Culicidae) throughout four different life stages

Feng¹,

Wu²,

Zhou³

et al. 2018

Cell Biosci

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BackgroundmicroRNAs (miRNAs) are one kind of small non-coding RNAs widely distributed in insects. Many studies have shown that miRNAs play critical roles in development, differentiation, apoptosis, and innate immunity. However, there are a few reports describing miRNAs in Anopheles sinensis, the most common, and one of the dominant malaria mosquito in China. Here, we investigated the global miRNA expression profile across four different developmental stages including embryo, larval, pupal, and adult stages using Illumina Hiseq 2500 sequencing.ResultsIn total, 164 miRNAs were obtained out of 107.46 million raw sequencing reads. 99 of them identified as known miRNAs, and the remaining 65 miRNAs were considered as novel. By analyzing the read counts of miRNAs in all developmental stages, 95 miRNAs showed stage-specific expression (q < 0.01 and |log2 (fold change)| > 1) in consecutive stages, indicating that these miRNAs may be involved in critical physiological activity during development. Sixteen miRNAs were identified to be commonly dysregulated throughout four developmental stages. Many miRNAs showed stage-specific expression, such as asi-miR-2943 was exclusively expressed in the embryo stage, and asi-miR-1891 could not be detected in larval stage. The expression of six selected differentially expressed miRNAs identified by qRT-PCR were consistent with our sequencing results. Furthermore, 5296 and 1902 target genes were identified for the dysregulated 68 known and 27 novel miRNAs respectively by combining miRanda and RNAhybrid prediction. GO annotation and KEGG pathway analysis for the predicted genes of dysregulated miRNAs revealed that they might be involved in a broad range of biological processes related with the development, such as membrane, organic substance transport and several key pathways including protein processing in endoplasmic reticulum, propanoate metabolism and folate biosynthesis. Thirty-two key miRNAs were identified by microRNA-gene network analysis.ConclusionThe present study represents the first global characterization of An. sinensis miRNAs in its four developmental stages. The presence and differential expression of An. sinensis miRNAs imply that such miRNAs may play critical roles in An. sinensis life cycle. A better understanding of the functions of these miRNAs will have great implication for the effective control of vector population and therefore interrupting malaria transmission.Electronic supplementary materialThe online version of this article (10.1186/s13578-018-0227-1) contains supplementary material, which is available to authorized users.

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Genetic diversity and population structure of the primary malaria vector Anopheles sinensis (Diptera: Culicidae) in China inferred by cox1 gene

Cited by 15 publications

References 39 publications

Sequencing and analysis of the complete mitochondrial genome in Anopheles sinensis (Diptera: Culicidae)

Sequencing and analysis of the complete mitochondrial genome in Anopheles sinensis (Diptera: Culicidae)

Biology, Bionomics and Molecular Biology of Anopheles sinensis Wiedemann 1828 (Diptera: Culicidae), Main Malaria Vector in China

Characterization and potential role of microRNA in the Chinese dominant malaria mosquito Anopheles sinensis (Diptera: Culicidae) throughout four different life stages

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