Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital

Canela, Heliara Maria Spina; Cardoso, Bárbara Rita; Frazão, Miliane Rodrigues; Falcão, Juliana Pfrimer; Vitali, Lúcia Helena; Martínez, Roberto; Ferreira, Márcia Eliana da Silva

doi:10.1007/s42770-021-00446-x

Cited by 4 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with our findings, several studies performed in Japan, South Korea, and China also indicated that ST7 is the major genotype associated with C. glabrata ( Dodgson et al, 2003 ; Hou et al, 2017 ; Byun et al, 2018 ; Canela et al, 2021 ). Conversely, Boonsilp et al (2021) have shown that the most common ST is ST55 (60%), followed by ST7 (20%), ST195 (10%), and ST199 (10%), among C. glabrata isolated from blood samples in Bangkok, Thailand.…”

Section: Discussionsupporting

confidence: 92%

“…A study performed by Canela et al (2021) showed lower genetic variability in C. glabrata obtained from a Brazilian hospital than in other Candida species. Using MLST, Boonsilp et al (2021) found that C. glabrata isolated from a tertiary care hospital in Bangkok, Thailand, shows a low level of genetic diversity and that the most common genotype is ST55 (60%), followed by ST7 (20%), ST195 (10%), and ST199 (10%).…”

Section: Introductionmentioning

confidence: 92%

“…The purpose of genotyping is to track the source of infection, clarify the route of transmission, and identify outbreaks. Numerous publications have reported several methods for genotyping, including multilocus sequence typing (MLST), restriction fragment length polymorphism, pulsed-field gel electrophoresis (PFGE), and microsatellite analysis ( Kiasat et al, 2019 ; Khalifa et al, 2020 ; Canela et al, 2021 ; Dikmen et al, 2021 ). Among these techniques, MLST, with its high reproducibility and reliability, is frequently used for strain phylogeny and epidemiology comparisons worldwide ( Canela et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multilocus Sequence Typing and Antifungal Susceptibility of Vaginal and Non-vaginal Candida glabrata Isolates From China

Chen

Lulou

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Candida glabrata is a common cause of Candida infections. In our present study, we investigated the antifungal susceptibility and molecular epidemiology of vaginal and non-vaginal C. glabrata isolates. Seventy-six vaginal C. glabrata strains isolated from patients with vulvovaginal candidiasis and 57 non-vaginal C. glabrata isolates were collected at two hospitals in Shanghai, China. Antifungal susceptibility was examined using a broth microdilution method. Multilocus sequence typing was used for genotyping. Overall, 28 (21.1%), 28 (21.1%), and 29 (21.8%) C. glabrata isolates were resistant to fluconazole, itraconazole, and voriconazole, respectively. Briefly, 18 (23.7%), 18 (23.7%), and 19 (25%) vaginal strains were resistant to fluconazole, itraconazole, and voriconazole. While the resistance to these antifungals were all 17.5% (10/57) in non-vaginal strains. All isolates retained susceptibility to amphotericin B, and only four non-vaginal isolates were caspofungin resistant. Genotyping identified 17 ST patterns. In non-vaginal samples, the same genotypes appear as in the vaginal samples, except for one genotype (ST-182), while in the vaginal samples more genotypes appear (ST8, ST19, ST45, ST55, ST66, ST80, ST138, and ST17). The most common genotype was ST7 (81 strains), followed by ST10 (14 strains) and ST15 (11 strains). The majority of resistant phenotype strains (25/30, 83.3%) correlated to the predominant genotype (ST7), and the rest belonged to ST3 (2/30, 6.7%), ST10 (1/30, 3.3%), ST19 (1/30, 3.3%), and ST45 (1/30, 3.3%). Our survey revealed cross-resistance in vaginal and non-vaginal C. glabrata isolates. Moreover, there is no genotype associated with the resistance phenotype.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multilocus Sequence Typing and Antifungal Susceptibility of Vaginal and Non-vaginal Candida glabrata Isolates From China

Chen

Lulou

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Microorganisms of the Candida genus are the main agents that cause fungal infections in the bloodstream, known as candidemia. It causes mortality rates varying from 15 to 35% and increases the period and costs of hospitalization, making the disease a serious public health problem, especially in developing countries (Canela et al, 2021). C. albicans and C. tropicalis are found in the microbiota of the reproductive and gastrointestinal mucosa, living symbiotically in about 50 -70% of healthy individuals (Megri et al, 2020).…”

Section: Fungal Strain Code Labmicramentioning

confidence: 99%

Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

Oliveira

Santiago

Katak

et al. 2021

RSD

View full text Add to dashboard Cite

Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis. In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.

show abstract

Molecular epidemiology and antimicrobial resistance of vaginal Candida glabrata isolates in Namibia

Dunaiski,

Kock,

Chan

et al. 2024

Medical Mycology

View full text Add to dashboard Cite

Candida glabrata is the most common non-albicans Candida species that causes vulvovaginal candidiasis (VVC). Given the intrinsically low susceptibility of C. glabrata to azole drugs, investigations into C. glabrata prevalence, fungal susceptibility profile, and molecular epidemiology are necessary to optimise the treatment of VVC. This molecular epidemiological study was conducted to determine antifungal drug profile, single nucleotide polymorphisms (SNPs) associated with phenotypic antifungal resistance and epidemic diversity of C. glabrata isolates from women with VVC in Namibia. Candida glabrata isolates were identified using phenotypic and molecular methods. Antifungal susceptibility of strains was determined for fluconazole, itraconazole, amphotericin B and anidulafungin. Whole genome sequencing was used to determine SNPs in antifungal resistance genes and sequence type (ST) allocation. Among C. glabrata isolates, all (20/20; 100%) exhibited phenotypic resistance to the azole class antifungal drug, (fluconazole), and phenotypic susceptibility to the polyene class (amphotericin B), and the echinocandins (anidulafungin). Non-synonymous SNPs were identified in antifungal resistance genes of all fluconazole-resistant C. glabrata isolates including ERG6 (15%), ERG7 (15%), CgCDR1 (25%), CgPDR1 (60%), SNQ2 (10%), FKS1 (5.0%), FKS2 (5.0%), CgFPS1 (5.0%) and MSH2 (15%). ST15 (n = 8/20, 40%) was predominant. This study provides important insight into phenotypic and genotypic antifungal resistance across C. glabrata isolates from women with VVC in Namibia. In this study, azole resistance is determined by an extensive range of SNPs, while the observed polyene and echinocandin resistance-associated SNPs despite phenotypic susceptibility require further investigation.

show abstract

Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital

Cited by 4 publications

References 47 publications

Multilocus Sequence Typing and Antifungal Susceptibility of Vaginal and Non-vaginal Candida glabrata Isolates From China

Multilocus Sequence Typing and Antifungal Susceptibility of Vaginal and Non-vaginal Candida glabrata Isolates From China

Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

Molecular epidemiology and antimicrobial resistance of vaginal Candida glabrata isolates in Namibia

Contact Info

Product

Resources

About