Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

Havlíčková, Lenka; Jozová, Eva; Rychla, Andrea; Klíma, Miroslav; Kučera, V.; Čurn, Vladislav

doi:10.17221/220/2013-cjgpb

Cited by 14 publications

(7 citation statements)

References 32 publications

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“…55 Likewise, RAPD, AFLP, ISSR, and SSR have been used to find the genetic variability in rapeseed. [56][57][58][59] Detailed information will be presented in the subsequent sections (2.1.1.1-2.1.1.7) to highlight the efforts in rapeseed mapping using different approaches.…”

Section: Marker-assisted Breedingmentioning

confidence: 99%

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

et al. 2021

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Plant abiotic stresses negative affects growth and development, causing a massive reduction in global agricultural production. Rapeseed (Brassica napus L.) is a major oilseed crop because of its economic value and oilseed production. However, its productivity has been reduced by many environmental adversities. Therefore, it is a prime need to grow rapeseed cultivars, which can withstand numerous abiotic stresses. To understand the various molecular and cellular mechanisms underlying the abiotic stress tolerance and improvement in rapeseed, omics approaches have been extensively employed in recent years. This review summarized the recent advancement in genomics, transcriptomics, proteomics, metabolomics, and their imploration in abiotic stress regulation in rapeseed. Some persisting bottlenecks have been highlighted, demanding proper attention to fully explore the omics tools. Further, the potential prospects of the CRISPR/Cas9 system for genome editing to assist molecular breeding in developing abiotic stress-tolerant rapeseed genotypes have also been explained. In short, the combination of integrated omics, genome editing, and speed breeding can alter rapeseed production worldwide.

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Section: Marker-assisted Breedingmentioning

confidence: 99%

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

et al. 2021

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“…A higher WAM value corresponded with the pattern of genotype distribution after factorial (PCoA) analysis (Figure 1b). In our previous study, focused on Brassica napus [32], we observed WAM values that were significantly lower (22.50% for SSR markers). These results showed the importance of extensive collections of genetic resources for preserving genetic diversity.…”

Section: Microsatellite Polymorphism and Genetic Diversity Of Genetic...mentioning

confidence: 66%

“…Bayesian clustering algorithms were performed in the STRUCTURE 2.3.4 software to describe the genetic structure better and to explain the results from previous statistical analyses. Although this approach has been highly frequented in ecological studies [38] in the last period, it is also used for agricultural crops including oil seed brassicas, such as B. napus [32], B. juncea [23], and B. carinata [25]. Through the STRUCTURE software, we determined the likelihood of the data for several different K, with K ranging from one to seven, to eventually see the plateau pattern in the diagnostic plot.…”

Section: Genetic Structure Of Yellow Mustard Varietiesmentioning

confidence: 99%

Microsatellite Markers: A Tool to Assess the Genetic Diversity of Yellow Mustard (Sinapis alba L.)

Jozová,

Rost,

Rychlá

et al. 2023

Plants

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Microsatellite markers were used for the assessment of genetic diversity and genetic structure in a germplasm collection of yellow mustard, Sinapis alba L. The comprehensive collection of genetic resources represented 187 registered varieties, landraces, and breeding materials. Microsatellites generated 44 polymorphic alleles in 15 loci. Eleven of them were medium to highly polymorphic, and the high levels of observed heterozygosity (0.12–0.83) and Nei’s gene diversity index (0.11–0.68) indicated a high level of polymorphism. Based on PCoA and neighbor joining analyses, the genetic resources were divided into two groups. The range of genetic dissimilarity in the analysed collection was in the range of 0.00–1.00. The high level of dissimilarity between the accessions was documented by the high WAM value (33.82%). Bayesian clustering algorithms were performed in the STRUCTURE 2.3.4 software. The number of clusters was estimated at K = 2. The accessions were classified according to Q1/Q2 values. The low average values of the parameters Fst_1 (0.3482), Fst_2 (0.1916), and parameter alpha (0.0602) indicated substantial mating barriers between varieties and reproductive isolation due to the limited exchange of genetic resources between breeders. These results demonstrated the importance of extensive collections of genetic resources for the maintenance of genetic diversity and indicated considerable genetic differentiation among accessions.

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“…In the past, it usually took one year to identify seed purity through field planting. In order to shorten the identification time, molecular markers such as RAPD and SSR have been applied to the identification of hybrid purity [ 25 , 26 ]. However, these markers usually only correspond to a specific variety, and the change in variety requires the re-development of molecular markers.…”

Section: Resultsmentioning

confidence: 99%

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Zhang

Song

et al. 2023

Plants

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Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize the heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about the global revolution of hybrid varieties, replacing conventional crop varieties. Over the last half century, China has led the development of hybrid Brassica napus varieties. Two sterile lines, polima (pol) and shaan 2A, were of particular importance for the establishment of three-line hybrid systems in rapeseed, which has opened up a new era of heterosis utilization. However, in current breeding practices, it takes up to three years to identify the restorer or maintainer relationship and the cytoplasmic type of any inbred material. This greatly affects the breeding speed of new varieties and inhibits the rapid development of the rapeseed industry. To address this problem, we developed a set of molecular markers for the identification of fertile cytoplasmic gene N and sterile cytoplasmic gene S, as well as for the fertile nucleus gene R and sterile nucleus gene r, based on differences in the gene sequences between the CMS line, maintainer line and restorer line of Brassica napus. Combining these markers can accurately identify the CMS line, maintainer and restorer of both the pol and shaan systems, as well as their hybrids. These markers can not only be used to identify of the maintainer and restorer relationship of inbred materials; they can also be used as general molecular markers to identify the CMS-type hybrid purity of pol and shaan systems.

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Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

Cited by 14 publications

References 32 publications

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

Microsatellite Markers: A Tool to Assess the Genetic Diversity of Yellow Mustard (Sinapis alba L.)

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

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