Genetic Diversity Between Two Populations of Heteropneustes fossilis (Bloch) Using RAPD Profile

Garg, R. K.; Sairkar, Pramod; Silawat, N.; Vijay, Neetu; Batav, Nitin; Mehrotra, Nitin

doi:10.3923/ijzr.2009.171.177

Cited by 17 publications

(15 citation statements)

References 9 publications

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“…This is consistent with the report of Daniel (1997), Ponnian and Gopalakrishnana (2000) and Garg et al (2009b). RAPD method uncovered 30 polymorphic loci from five primers selected for population analysis.…”

Section: Discussionsupporting

confidence: 81%

Genetic Differentiation Studies among Natural Populations of <i>Tilapia zillii</i>

2015

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The population structure of Tilapia zillii (Gervais 1848) from three reservoirs in Nigeria, Osun State (Opa, Osu and Igun) was determined by employing morphological and molecular (Random Amplified Polymorphic DNA) methods. For morphological studies, 25 morphometric measurements and six meristic counts were recorded on 40 individuals within each population. Principal Component Analysis (PCA) was performed on the morphometric and meristic data using the PAST software. For RAPD studies, genomic DNA was extracted from caudal fin tissue using CTAB method and five primers were used to initiate PCR amplifications. All the clusters produced by the Principal components analysis (PCA) of the morphometric and meristic parameters overlapped indicating a low level of genetic differentiation between the three populations of T. zillii studied. The UPGMA cluster diagram from RAPD analysis identified two major genotypic groups with inter and intra group relationships. All individuals in the first cluster were from the Osu reservoir, while individuals from Opa and Igun reservoirs constituted the second cluster. Nei's unbiased measure of genetic distances was 0.8532, 0.7321 and 0.7111 for Osu, Igun and Opa populations respectively. This revealed that Opa and Igun populations were genetically closer, while Osu populations is distant from them. The results suggest that the RAPD technique could be used to differentiate populations of T. zillii. However, additional methods such as microsatellite and sequence analysis can be used to maximize the efficiency of genetic differentiation studies.

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Section: Discussionsupporting

confidence: 81%

Genetic Differentiation Studies among Natural Populations of <i>Tilapia zillii</i>

2015

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“…3). The morphometric results are insignificant to support the established genetic structure of the population that often leads to taxonomic uncertainty (Daniel, 1997;Ponnian and Gopalakrishnana, 2000;Garg et al, 2009b). The investigation was further extended to analyze the genetic variation.…”

Section: Resultsmentioning

confidence: 99%

Assessment of Morphometric and Genetic Variation in Three Freshwater Fish Species of the Genus Garra (Osteichthyes: Cyprinidae)

et al. 2011

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The present study evaluated the patterns of morphometric and genetic variation using RAPD-PCR techniques for the first time on three species of Garra, viz. G. mullya, G. kalakadensis and G. gotyla stenorhynchus, collected from various river basins of South-India. The results of morphological analysis revealed that G. mullya and G. kalakadensis hold many similar characters compared to the other congener, G. gotyla stenorhynchus. However, the G. gotyla stenorhynchus fish species exhibited distinct variation in the morphological characters such as snout length, pre-nasal length, inter-nasal width, gap width, lower jaw to isthmus, head depth at pupil, dorsal fin length and disc width from the other two species of Garra. However, certain morphometric characters overlapped. Hence the RAPD finger printing was used to assess the levels of genetic variation in Garra spp. using RAPD-PCR technique. A total of 72 reliable fragments were detected using 10 Operon primers, ranging from 2600 molecular weight to 3100. The shared RAPD fragments found in both G. mullya and G. kalakadensis with fixed frequencies were observed with all the investigated primers, implying their genetically closer relationship. However, the similarity index observed for G. gotyla stenorhynchus was less with the other two species specifying a genetically distant link. The present investigation thus contribute to the knowledge on morphological and genetic variation in these three Garra species.

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“…RAPD banding patterns were recorded on spreadsheets, which were used to determine gene diversity, gene flow, number of polymorphic loci and genetic distance characteristics of the fishes in the present study were similar and there is overlap in the range of each of traditional morphometric measurements taken (Tables 2 and 3). The morphometric data may not be enough to support the established genetic structure of the population that often leads to taxonomic uncertainity in many occasions because of the considerable geographical and ecological variability in form [25,26]. In Hilsa sp., significant differences in allele frequencies and morphological variations were observed from nine different sites within Bangladesh by which may be due to the local environmental conditions [27].…”

Section: Discussionmentioning

confidence: 99%

Genetic Variation among Cat Fish (Mystus vittatus) Population Assessed by Randomly Amplified Polymorphic (RAPD) Markers from Assam, India

Hasan¹,

Goswami²

2015

J Aquac Res Development

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Genetic Diversity Between Two Populations of Heteropneustes fossilis (Bloch) Using RAPD Profile

Cited by 17 publications

References 9 publications

Genetic Differentiation Studies among Natural Populations of <i>Tilapia zillii</i>

Genetic Differentiation Studies among Natural Populations of <i>Tilapia zillii</i>

Assessment of Morphometric and Genetic Variation in Three Freshwater Fish Species of the Genus Garra (Osteichthyes: Cyprinidae)

Genetic Variation among Cat Fish (Mystus vittatus) Population Assessed by Randomly Amplified Polymorphic (RAPD) Markers from Assam, India

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