Genetic diversity for random amplified polymorphic DNA (RAPD) markers in two Swedish populations of Pyrenophora teres

Jönsson, Rickard; Säll, Torbjörn; Bryngelsson, Tomas

doi:10.1080/07060660009500473

Cited by 33 publications

(34 citation statements)

References 14 publications

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“…The sequencing of PCR products showed that at least one of the published SNPs (C / T-384 bp) does not occur in Czech Pyrenophora teres isolates. These results correspond to the fact that P. teres shows very high intraspecific variability (Peltonen et al, 1996;Minarikova & Polisenska, 1999;Jonsson et al, 2000). This problem could also refer to two different AFLP fragments, CTT/AG (339 bp) and CTT/AG (348 bp), which showed very high sequence homology (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years DNA technology has become one of the major tools for the identification and detection of fungal plant pathogens (Mugnier, 1995). Most published works concerning the study of Pyrenophora isolate variability have been based on the analysis of ITS regions (Stevens et al ., 1998), on molecular methods like random amplified polymorphic DNA (RAPD; Peever & Milgroom, 1994;Peltonen et al ., 1996;Campbell et al ., 1999;Jonsson et al ., 2000) or restriction fragment length polymorphism (RFLP; Louw et al ., 1995;Wu et al ., 2002). Taylor et al .…”

Section: Introductionmentioning

confidence: 99%

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AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

2005

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Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres , the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of Pyrenophora species. The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis , P. graminea and Helminthosporium sativum .

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

2005

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“…Low levels of genetic differentiation have been observed between fields located close to each other (e.g. within 20 km) (Peever & Milgroom 1994;Jonsson et al 2000;Serenius et al 2007), whereas fields separated by greater distances (e.g. between different countries, states, or continents) have shown a higher level of genetic differentiation (Serenius et al 2007).…”

Section: Introductionmentioning

confidence: 97%

“…The genetic structure of P. teres populations has previously been investigated using random amplified polymorphic DNA (RAPD) (Peever & Milgroom 1994;Jonsson et al 2000;Campbell et al 2002) and amplified fragment length polymorphism (AFLP) (Rau et al 2003;Leisova et al 2005b;Serenius et al 2007) molecular markers. In these studies, varying levels of genetic diversity were detected among populations, with geographical distance shown to be a factor in determining the level of genetic differentiation between populations.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of South Australian Pyrenophora teres populations as revealed by microsatellite analyses

2010

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“…Robinson and Jalli (1997) reported that partial resistance and the average effect of alleles can be inherited and that these may be important factors in conditioning resistance to net blotch. Screening for resistance to net blotch have been conducted for several decades (Jonsson et al, 1997;Jalli and Robinson, 2000). Such studies are limited in their usefulness as different experiments examined different genotypes.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic variation in populations of Drechslera teres f. teres and D. teres f. maculata and differences in host cultivar responses

et al. 2006

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The current study examined the variability in the pathogenicity of populations of Drechslera teres f. teres and D. teres f. maculata (the net and spot forms of D. teres) from Ireland and northern Europe. A population of progeny isolates from a mating of net and spot forms was also examined. Significant variation in virulence was found both between and among net form and spot form isolates (p<0.001). In the Irish population, significant differences were found between the net and spot forms, with the spot form isolates more virulent (p<0.05). Progeny isolates were significantly more virulent than net form or spot form populations (p<0.001). Significant differences were found in cultivar reactions, with cv. Botnia most susceptible to both forms of the pathogen (p<0.001). Cultivar Boreal 94145, although quantitatively resistant, was found to be very susceptible to both forms of the pathogen and to progeny isolates. Cultivars CI 5791, CI 2330 and CI 9819 were all less susceptible to infection by both forms, but were more susceptible to spot form isolates. Significant correlations were found between whole plants and detached leaf experiments for the net form isolates only (p<0.001). This study illustrates the importance of including both net form and spot form isolates in resistance studies and the need for a clearer understanding for the genetic basis of resistance to the net and spot forms. It also highlights the limitations of using a detached leaf assay for screening of net blotch of barley.

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Genetic diversity for random amplified polymorphic DNA (RAPD) markers in two Swedish populations of Pyrenophora teres

Cited by 33 publications

References 14 publications

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

Genetic structure of South Australian Pyrenophora teres populations as revealed by microsatellite analyses

Pathogenic variation in populations of Drechslera teres f. teres and D. teres f. maculata and differences in host cultivar responses

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