Genetic diversity in common carp stocks assayed by random-amplified polymorphic DNA markers

Abstract

The common carp (Cyprinus carpio L.) is one of the major aquaculture species, contributing nearly 35% to the inland fish production in Karnataka, India. Stocks collected from Hungary (2), Indonesia and Vietnam were assessed alongside two local stocks in a series of culture performance trials with the objective of setting up a base population for developing a breeding programme. The present study deals with the genetic divergence and polymorphism in these six stocks using random-amplified polymorphic DNA (RAPD) markers. A total of 180 decamer random primers were screened for polymerase chain reaction (PCR) amplification (OPA 1-20, OPB1-20, OPC1-20, OPD1-20, OPE1-20, OPF1-20, OPG1-20, OPP1-20 and OPM1-20). Eight primers were selected for analysis of common carp genotypes (OPA-7, OPA-20, OPB-17, OPF-10, OP F-9, OPG-4, OPG-9 and OPP-16). Out of 492 bands recorded, 57.1% were polymorphic. Stepwise regression analysis was carried out to find best combination markers affecting body weight (P < 0.001). The results demonstrate major differences in the genetic structures between different stocks. Dendrogram data showed grouping of individuals according to stocks and corresponding data variables revealed the per cent homology within the stock and also found markers correlating to the body weigh

Comparación de protocolos de extracción de ADN con muestras de aleta y larva de peces: extracción modificada con cloruro de sodio

“…Además, es destacable su facilidad de colecta y confi abilidad, en comparación con muestras de músculo o sangre. La aleta del pez y la larva son fuentes confi ables de ADN y han sido usadas exitosamente en varios estudios poblacionales y taxonómicos (Vieira et al, 2005;Povh et al, 2005;Aho et al, 2006;Basavaraju et al, 2007), incluyendo el muestreo de descendencias de piscícolas o de aquellas usadas en programas de mejoramiento y repoblamiento (LoperaBarrero, 2005; Povh, 2007;Gomes, 2007).…”

Optimizing reproducibility evaluation for random amplified polymorphic DNA markers

“…We also analyzed the same data sets using the POPGENE 1.32 program (population genetic analysis) (Yeh and Boyle, 1997) and estimated the proportion of polymorphic loci (P%), Nei's genetic diversity (He) and Shannon genetic diversity (S), assuming Hardy-Weinberg equilibrium. Although these estimates are not entirely adequate for dominant markers, they are still widely used (e.g., Artiukova et al, 2004;Ferreyra et al, 2004;Basavaraju et al, 2007) …”

Utilización De La Biología Molecular Como Medio Para Optimizar La Producción Piscícola Y Repoblamiento De Medios Naturales

“…En peces, para obtener muestras adecuadas de ADN se han utilizado aletas, sangre, tejido hepático, escamas, óvulos, células bucales, larvas y músculo entre otros (CUMMINGS & THORGAARD, 1994;RAMÍREZ-GIL, 2001;GALLO & DÍAZ-SARMIENTO, 2003;WASKO et al,2003;ARANISHI, 2006;CHAKRABORTYet al,2006;LIVIA et al,2006). Los cuales han sido usados con los diferentes marcadores moleculares, para determinar estudios poblacionales, filogenia, taxonómico, muestreos de descendencia para programas de mejoramiento y repoblamiento (WASKOet al,et al,2003;LOPERA-BARRERO, 2005;POVH et al, 2005;VIEIRA et al,2005;AHO et al,2006;BASAVARAJU et al,2007;GOMES, 2007), así POVH et al (2007)…”