GENETIC DIVERSITY IN KAYU MERAH (Pterocarpus indicus Willd) POPULATIONS USING RANDOM AMPLIFIED POLYMORPHISM DNA MARKER

Sulistyawati, Purnamila; Widyatmoko, Aypbc

doi:10.20886/jpth.2017.11.1.67-76

Cited by 13 publications

(7 citation statements)

References 5 publications

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“…DNA samples capable of producing bands indicate that the DNA has a complementary sequence with the primer. This difference can indicate genetic diversity both within and among populations (Sulistyawati and Widyatmoko 2017).…”

Section: Discussionmentioning

confidence: 99%

Genetic variation and characterization of the sucrose synthase 2 gene (Sus2) in sugarcane based molecular markers

Kasiamdari

Febiansi

Prabowo³

et al. 2019

Biodiversitas

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Abstract. Kasiamdari RS, Febiansi D, Prabowo H, Aristya GR, Musthofa A. 2019. Genetic variation and characterization of the sucrose synthase 2 gene (Sus2) in sugarcane based molecular markers. Biodiversitas 20: 3087-3096. Sugarcane (Saccharum spp.) genotype is one of the sugar-producing plants and an important commodity to support the community’s economy. Plant breeders continuously cross-breed these plants to obtain cultivars with desirable traits to produce a variety of new phenotypes. An analysis of variation at gene-level is carried out as an important step in plant breeding programs. The purpose of the present study was to determine genetic variation and characterize the sucrose synthase 2 gene (Sus2) based on molecular markers. Twenty sugarcane cultivars were sampled from Indonesian Sweetener and Fiber Crops Research Institute (BALITTAS) Malang and four sugarcane cultivars from PT. Madu Baru in Polosio A Plantation, Poncosari, Srandakan, Bantul Regency, Yogyakarta. DNAs were amplified using two primer pairs, i.e. AI and SMC226CG. The genetic variations of the twenty-four sugarcane cultivars were analyzed by constructing a dendrogram of the Amplified DNA using Multi-Variate Statistical Package (MVSP) software. The results showed that there were three large clusters, namely Cluster A consisting of ‘PS 865’, ‘PS 951’, ‘PS 921’, and ‘PS 58’, with a similarity index of 57%; Cluster B consisting of the sugarcane ‘Kentung’, with a similarity index of 33%; and cluster C consisting of the sugarcane ‘PSDK 923’, ‘PSBM 901’, ‘TLH 2’, ‘BL’, ‘PSJT 941’, ‘KK’, ‘PS 80.1649’, ‘PSCO 902’, ‘PS 80,910’, ‘PS 882’, ‘PS 862’, ‘PS 851’, ‘PS 881’, ‘PS 865’, ‘PS 384’, ‘VMC 76-16’, ‘BZ 132’, ‘PS 891’, ‘PS 41’ with a similarity index of 60%. Polymorphisms occurred after the DNAs were amplified and after the bands appeared in heterozygous and homozygous individuals. In sugarcane, the Sus2 gene when detected by the molecular marker primer AI showed high-sucrose sugarcane; whereas the Sus2 gene detected by the primer SMC226CG showed low-sucrose sugarcane. Results of the present study showed that all sugarcane samples were detected as having low sucrose except Bulu Lawang and Kentung cultivars.

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Section: Discussionmentioning

confidence: 99%

Genetic variation and characterization of the sucrose synthase 2 gene (Sus2) in sugarcane based molecular markers

Kasiamdari

Febiansi

Prabowo³

et al. 2019

Biodiversitas

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“…Different trends are seen in Gowa populations that come from the central region but have a genetic distance closer to the populations of the western regions. Similarly, (Sulistyawati and Widyatmoko 2017) reported that the genetic distance of Timor Island's redwood population is closer to the redwood population of Seram Island compared to the population of Flores Island, which is geographically closer.…”

Section: Genetic Distance and Relationshipmentioning

confidence: 93%

Genetic diversity of sugar palm (Arenga pinnata) derived from nine regions in Indonesia based on SSR markers

et al. 2021

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Abstract. Rinawati DY, Reflinur, Dinarti D, Sudarsono. 2021. Genetic diversity of sugar palm (Arenga pinnata) derived from nine regions in Indonesia based on SSR markers. Biodiversitas 22: 3749-3755. Sugar palm (Arenga pinnata (Wurmb) Merr.) has an important economic and conservation value. Indonesia has genetic diversity potential of sugar palm, considering the widespread distribution of sugar palm in Indonesia which has variations in geographical type. This study aims to determine the diversity and relationship of sugar palm from nine regions in Indonesia based on SSR markers. The genetic material consists of 141 sugar palm accessions derived from Bangka, Lampung, Lebak, Bogor, Tasikmalaya, Brebes, Gowa, Bombana, and Muna. Nine pairs of SSR primers were used for genotyping. The highest and lowest genetic diversity was found in the Bangka and Muna populations, respectively. The genetic diversity within a population (79%) was higher than the genetic diversity between populations (21%). The genetic distance between Bangka and Lebak is the closest (0.033), while between Lampung and Muna is the farthest (0.283). The accession relationship is divided into three major clusters. Clusters 1 consisted of Bangka, Lampung, Bogor, Tasikmalaya, Brebes, Gowa, Bombana and Muna accessions. Cluster 2 consisted of Bangka, Lampung, Lebak, Bogor, Tasikmalaya, Brebes, and Gowa accessions. Cluster 3 consisted of Bangka, Lebak, Brebes, Tasikmalaya, and Gowa accessions. Accession clustering does not show a typical relationship pattern based on geographic location.

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“…dieksploitasi cukup tinggi oleh masyarakat. Namun hal ini tidak diiringi dengan kemampuan regenerasi yang baik, sehingga mengakibatkan penurunan populasi (Sulistyawati & Widyatmoko, 2017). Hal ini telah terjadi selama bertahun-tahun karena penebangan yang tidak selektif dan hilangnya habitat secara keseluruhan.…”

Section: Pendahuluanunclassified

Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro

Nofitria,

Violita,

Novita

et al. 2024

JBT

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Pterocarpus indicus Willd. also known as redwood is a native Philippine species belonging to the Fabaceae family that has many uses and high economic value and highly exploited. However, it is not accompanied by good regeneration which causes this species to be categorized as endangered (IUCN v. 2021-1). The in vitro technique is an alternative to overcome this problem. This study aims to determine the response of P. indicus Willd. shoot growth to the addition of BAP and TDZ in vitro. The research was conducted from August to December 2023, at the Biotechnology Laboratory, KST BJ. Habibie Puspiptek Serpong, South Tangerang, Banten. This study used a completely randomized design (CRD) consisting of 9 treatments and 14 replications. The treatments consisted of: R0 = Control, R1 = BAP 0,25 ppm, R2 = BAP 0,5 ppm, R3 = BAP 0,75 ppm, R4 = BAP 1 ppm, R5 = TDZ 0,25 ppm, R6 = TDZ 0,5 ppm, R7 = TDZ 0,75 ppm, R8 = TDZ 1 ppm. The results showed that the concentration of BAP 0,5 ppm gave the best results in height of shoot and TDZ 0,25 ppm gave optimum results in the number of shoots compared to the control and other treatment concentrations.

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GENETIC DIVERSITY IN KAYU MERAH (Pterocarpus indicus Willd) POPULATIONS USING RANDOM AMPLIFIED POLYMORPHISM DNA MARKER

Abstract: Kayu merah (Pterocarpus indicus Willd) which listed as one of the IUCN Red List of Threatened Species in the category Vulnerable (VU A1D) is naturally distributed throughout Indonesia included Java,

Cited by 13 publications

References 5 publications

Genetic variation and characterization of the sucrose synthase 2 gene (Sus2) in sugarcane based molecular markers

Genetic variation and characterization of the sucrose synthase 2 gene (Sus2) in sugarcane based molecular markers

Genetic diversity of sugar palm (Arenga pinnata) derived from nine regions in Indonesia based on SSR markers

Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro

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