Genetic Diversity in &lt;i&gt;Streptococcus dysgalactiae&lt;/i&gt; subsp. &lt;i&gt;equisimilis&lt;/i&gt; Isolates from Patients with Invasive and Noninvasive Infections in a Japanese University Hospital (2014&amp;ndash;2015)

Fujita, Takeshi; Horiuchi, Ayaka; Ogawa, Miho; Yoshida, Haruno; Hirose, Yoshio; Nagano, Noriyuki; Takahashi, Takashi

doi:10.7883/yoken.jjid.2015.602

Cited by 7 publications

(13 citation statements)

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“…The emm gene, encoding the M protein, is widely used for molecular epidemiology studies of these two organisms [ 2 ]. Multilocus sequence typing (MLST) has enhanced understanding of the genetic diversity of SDSE [ 13 ]. Although SDSE is highly susceptible to β-lactam antibiotics, several reports have identified high resistance rates to classes of macrolides, lincosamides, or tetracycline [ 6 ].…”

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confidence: 99%

Molecular Epidemiological Features and Antibiotic Susceptibility Patterns of Streptococcus dysgalactiae subsp. equisimilis Isolates from Korea and Japan

et al. 2018

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BackgroundThe molecular characterization of Streptococcus dysgalactiae subsp. equisimilis (SDSE) has not yet been performed in Korea. This study aimed to find the differences or similarities in the clinical features, molecular epidemiological findings, and antimicrobial resistance patterns of SDSE from two countries (Korea and Japan).MethodsSDSE isolates were collected from Korea (N=69) from 2012–2016 and Japan (N=71) from 2014–2016. Clinical characteristics, emm genotypes, and sequence types (STs) were compared. Microdilution tests were performed using different antimicrobials, and their resistance determinants were screened.ResultsMedian ages were 69 years in Korea and 76 years in Japan. The most common underlying diseases were diabetes and malignancy. Blood-derived isolates comprised 36.2% and 50.7% of Korean and Japanese isolates, respectively; mortality was not different between the two groups (5.8% vs 9.9%, P=0.53). Among Korean isolates with 20 different combined ST-emm types, ST127-stG245 (N=16), ST128-stG485 (N=10), and ST138-stG652 (N=8) were prevalent. Among Japanese isolates with 29 different combined types, ST17-stG6792 (N=11), ST29-stG485 (N=7), and ST205-stG6792 (N=6) were prevalent. Resistance rates to erythromycin, clindamycin, and minocycline were 34.8%, 17.4%, and 30.4% in Korea and 28.2%, 14.1%, and 21.4% in Japan, respectively.ConclusionsSDSE infections commonly occurred in elderly persons with underlying diseases. There was a significant difference in the distribution of ST-emm types between the two countries. Antimicrobial resistance rates were comparable with different frequencies of resistance determinants in each country.

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Section: Introductionmentioning

confidence: 99%

Molecular Epidemiological Features and Antibiotic Susceptibility Patterns of Streptococcus dysgalactiae subsp. equisimilis Isolates from Korea and Japan

et al. 2018

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“…To date, only a few studies have examined the relationship between the presence of sicG and molecular epidemiological markers (e.g., emm genotypes, sequence types [STs], and antimicrobial resistance determinants) in SDSE isolates [14]. We aimed to determine the association between the presence of sicG and the microbiological and clinical features of SDSE isolates collected from three central prefectures (Saitama, Tokyo, and Chiba) in Japan over two years (2014–2016).…”

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confidence: 99%

Prevalence of Complement-Mediated Cell Lysis-like Gene (sicG) in Streptococcus dysgalactiae subsp. equisimilis Isolates From Japan (2014–2016)

et al. 2017

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BackgroundStreptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan.MethodsGroup G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data.; they were obtained from 25 sterile (blood, joint fluid, and cerebrospinal fluid) and 30 non-sterile (skin-, respiratory tract-, and genitourinary tract-origin) samples. emm genotyping, multilocus sequence typing, sicG amplification/sequencing, and random amplified polymorphic DNA (RAPD) analysis of sicG-positive strains were performed.ResultssicG was detected in 30.9% of the isolates (16 human and one canine) and the genes from the 16 human samples (blood, 10; open pus, 3; sputum, 2; throat swab, 1) and one canine sample (open pus) showed the same sequence pattern. All sicG-harboring isolates belonged to clonal complex (CC) 17, and the most prevalent emm type was stG6792 (82.4%). There was a significant association between sicG presence and the development of skin/soft tissue infections. CC17 isolates with sicG could be divided into three subtypes by RAPD analysis.ConclusionsCC17 SDSE harboring sicG might have spread into three closely-related prefectures in central Japan during 2014–2016. Clonal analysis of isolates from other areas might be needed to monitor potentially virulent strains in humans and animals.

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“…We collected six isolates with β-hemolytic groups G/C/A streptococcal infection at intervals over three weeks from a repository at the Clinical Laboratory of Kitasato University Medical Center from May 1, 2014 through April 30, 2017 as these isolates might cause repetitive infections. These isolates were identified as SDSE using an API-20 Strep system (Sysmex BioMérieux, Tokyo, Japan) for biochemical testing, followed by confirmation using PCR amplification of the 16S rRNA gene, as described previously [6]. The isolates were considered positive if the PCR-amplified product yielded at least one sequence showing ≥98.7% similarity with the 16S rRNA sequence of the type strain National Collection of Food Bacteria (NCFB) 1356(T).…”

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“…All isolates were subjected to emm genotyping, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and random amplified polymorphic DNA (RAPD) analyses, as described previously [67]. Briefly, all emm typing was based on the U.S. Centers for Disease Control and Prevention database (http://www2a.cdc.gov/ncidod/biotech/strepblast.asp).…”

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“…MLST was performed by sequencing seven housekeeping genes ( gki , gtr , murI , mutS , recP , xpt , and atoB ) according to the SDSE website (http://pubmlst.org/sdysgalactiae/). Sequence types (STs) were grouped into clonal complexes, in which related STs were classified as single locus variants differing in only one housekeeping gene [678]. PFGE profiling following DNA digestion with the restriction enzyme Sma I was also performed [6].…”

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Comparison of Characteristics of Streptococcus dysgalactiae subsp. equisimilis Isolates Causing Repetitive vs Single Infections

et al. 2019

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No study has described Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates that cause repetitive infections (recurrence and reinfection). We compared the microbiological characteristics of SDSE causing repetitive infections with those causing single infections. Three patients with invasive infections were identified based on their medical records, and multiple SDSE isolates were collected at intervals over three weeks, using a laboratory repository. Isolates from 12 patients with single-episode infections served as controls. Six isolates were collected from three patients with first and second episodes of infection. All isolates causing either repetitive or single-episode infection were subjected to emm typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and random amplified polymorphic DNA (RAPD) analyses. Amplification of five virulence genes ( sicG , prtF1 , prtF2 , lmb , and cbp ), biofilm formation (BF), and cell invasion abilities (CIAs) were measured as virulent phenotypes. We observed close genetic similarities in the data obtained by emm typing, MLST, PFGE, and RAPD in four isolates from two patients, suggesting recurrence, whereas two isolates from one patient indicated genetic differences in these data, suggesting re-infection. The presence of the five virulence genes and the BF and CIA measurements appeared not to contribute to repetitive infections, compared with isolates causing single-episode infection. In conclusion, clinicians encountering patients with repetitive infections should be aware of both possibilities: recurrence with closely related strains and reinfection with different strains.

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Genetic Diversity in <i>Streptococcus dysgalactiae</i> subsp. <i>equisimilis</i> Isolates from Patients with Invasive and Noninvasive Infections in a Japanese University Hospital (2014–2015)

Cited by 7 publications

References 15 publications

Molecular Epidemiological Features and Antibiotic Susceptibility Patterns of Streptococcus dysgalactiae subsp. equisimilis Isolates from Korea and Japan

Molecular Epidemiological Features and Antibiotic Susceptibility Patterns of Streptococcus dysgalactiae subsp. equisimilis Isolates from Korea and Japan

Prevalence of Complement-Mediated Cell Lysis-like Gene (sicG) in Streptococcus dysgalactiae subsp. equisimilis Isolates From Japan (2014–2016)

Comparison of Characteristics of Streptococcus dysgalactiae subsp. equisimilis Isolates Causing Repetitive vs Single Infections

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