Genetic diversity in populations of the medicinal plant Leonurus cardiaca L. revealed by inter-primer binding site (iPBS) markers

Borna, Fatemeh; Luo, Shuming; Ahmad, Nabil; Nazeri, Vahideh; Shokrpour, Majid; Trethowan, Richard

doi:10.1007/s10722-016-0373-4

Cited by 23 publications

(21 citation statements)

References 32 publications

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“…Thus far, the application of the iPBS method for investigating the genetic diversity of fungal pathogens has been very limited (Borna et al, 2016;Ozer & Bayraktar, 2018;Özer, Bayraktar & Baloch, 2016;Šķipars et al, 2018;Wu et al, 2019). To date, retrotransposon sequences have been used to study the genetic diversity of only a few Alternaria species isolated from wheat seeds.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Alternaria species associated with black point in wheat grains

Turzhanova

Хапилина

Tumenbayeva

et al. 2020

PeerJ

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The genus Alternaria is a widely distributed major plant pathogen that can act as a saprophyte in plant debris. Fungi of this genus frequently infect cereal crops and cause such diseases as black point and wheat leaf blight, which decrease the yield and quality of cereal products. A total of 25 Alternaria sp. isolates were collected from germ grains of various wheat cultivars from different geographic regions in Kazakhstan. We investigated the genetic relationships of the main Alternaria species related to black point disease of wheat in Kazakhstan, using the inter-primer binding site (iPBS) DNA profiling technique. We used 25 retrotransposon-based iPBS primers to identify the differences among and within Alternaria species populations, and analyzed the variation using clustering (UPGMA) and statistical approaches (AMOVA). Isolates of Alternaria species clustered into two main genetic groups, with species of A.alternata and A.tennuissima forming one cluster, and isolates of A. infectoria forming another. The genetic diversity found using retrotransposon profiles was strongly correlated with geographic data. Overall, the iPBS fingerprinting technique is highly informative and useful for the evaluation of genetic diversity and relationships of Alternaria species.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of Alternaria species associated with black point in wheat grains

Turzhanova

Хапилина

Tumenbayeva

et al. 2020

PeerJ

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“…Traditionally, genetic diversity analysis of date palm used morphological markers based on a few characteristics of the fruit (shape, weight, colour, skin aspect, consistency and texture), leaves and spines and pedigree information. However, analysis among closely related cultivars using morphological characters alone is often unreliable and influenced by the environment (Mehmood et al, 2013;Luo et al, 2015;Borna et al, 2016). Isozyme markers were used as an alternative, although the method was limited by the number of informative data and gave no direct assessment of DNA genomic variation (see Akbari et al 2012;Al-Najm et al, 2016;Khanam et al, 2012;Mehmood et al, 2014;Mehmood et al, 2016;Sedra et al, 1993;Sedra et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Molecular variability and population structure of a core collection of date palm (Phoenix dactylifera L.) cultivars from Australia and the Middle East

Al-Najm¹,

Luo²,

Ahmad³

et al. 2017

Aust J Crop Sci

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Understanding genetic relatedness in date palm (Phoenix dactylifera L.) germplasm is important for effective plant improvement. Microsatellite (SSR) markers were used to assess the molecular variation and population structure of 60 Australian locally selected and exotic genotypes (24 female, 30 male and 6 related species), 12 Iraqi female cultivars and 10 female cultivars from Jordan. The main objectives were to survey genetic diversity and determine population structure in this core date palm collection which includes the most important and widely distributed cultivars in Australia. These Australian accessions were then compared to those originating from the date palm center of origin in the Middle East. PCR of 17 SSR primers (co-dominant markers) produced a total of 313 alleles ranging from 5 to 31 with an average of 18.4 alleles per locus. The PIC value for these 17 primers ranged from 0.4771 to 0.8199 with a mean of 0.670. The mean expected heterozygosity (0.841), mean observed heterozygosity (0.946) and Shannon's information index (2.067) indicated a high level of genetic diversity among the accessions. Multi-locus DNA fingerprints based on the 17 SSR loci unambiguously differentiated all accessions and revealed an absence of duplicated samples. Ordination and cluster analyses showed that the Australian accessions did not group together geographically; instead separate male and female groups differentiated among the six clusters. A Bayesian cluster analysis also partitioned the accessions into six groups and this result was largely compatible with the result of ordination analysis. The Australian date palm germplasm is highly diverse and thus provides an effective platform for plant improvement, enhancement of date production and the conservation of genetic resources.

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“…The estimators of genetic diversity for B. veronicifolia were generated by iPBS markers and were similar to those reported for populations of Smallanthus sonchifolius [34], motherwort (Leonurus cardiaca L.) [35] and Cicer sp. [36].…”

Section: Discussionmentioning

confidence: 66%

Influence of Environmental Factors on the Genetic and Chemical Diversity of Brickellia veronicifolia Populations Growing in Fragmented Shrublands from Mexico

Pacheco‐Hernández

Villa‐Ruano

Lozoya‐Gloria

et al. 2021

Plants

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Brickellia veronicifolia is a native Asteraceae from Mexico that persists in fragmented habitats. This investigation reports the genetic and chemical diversity of B. veronicifolia. The diversity analysis based on iPBS markers showed an averaged Shannon index (S) of 0.3493, a Nei genetic diversity (h) of 0.2256, and a percentage of polymorphic loci average (P) of 80.7867%. The population structure obtained by AMOVA revealed that the highest variation found within populations was 94.58%. GC-MS profiling of six populations indicated that major volatiles were β–caryophyllene (11.63%), spathulenol (12.85%), caryophyllene oxide (13.98%), α–cadinol (7.04%), cubedol (6.72%) and tau-muurolol (4.81%). Mantel tests suggested a statistically significant relationship between minor volatiles and geographical distance (r = 0.6163; p = 0.0470; p ˂ 0.05). Likewise, major volatiles showed a significant correlation with the soil pH (r = 0.6862; p = 0.0230) and maximum temperature (r = 0.4999; p = 0.0280). Our study suggests that the variation and genetic divergence of B. veronicifolia has no relationship with climatic parameters, whereas the volatiles are probably influenced by environmental factors and not by the genotype per se. Based on the characteristics of B. veronicifolia, this plant could be considered as a candidate for restoring fragmented shrublands in Mexico.

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Genetic diversity in populations of the medicinal plant Leonurus cardiaca L. revealed by inter-primer binding site (iPBS) markers

Cited by 23 publications

References 32 publications

Genetic diversity of Alternaria species associated with black point in wheat grains

Genetic diversity of Alternaria species associated with black point in wheat grains

Molecular variability and population structure of a core collection of date palm (Phoenix dactylifera L.) cultivars from Australia and the Middle East

Influence of Environmental Factors on the Genetic and Chemical Diversity of Brickellia veronicifolia Populations Growing in Fragmented Shrublands from Mexico

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