Genetic diversity information of seagrass Enhalus acoroides and Cymodocea rotundata for the local genetic conservation at North Maluku

Ramili, Yunita; Bengen, Dietriech G.; Madduppa, Hawis; Kawaroe, Mujizat

doi:10.1088/1755-1315/584/1/012021

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Studies on seagrass genetic diversity in Indonesia are still limited to certain species. For example, studies on the population structure of E. acoroides (Putra et al 2018), S. isoetifolium, and T. hemprichii (Wainwright et al 2018) C. rotundata (Ramili et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Stevanus

Pharmawati

2020

Biodiversitas

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Abstract. Stevanus, Pharmawati M. 2021. Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia. Biodiversitas 22: 50-57. West Lombok, Indonesia is one of the locations that is thought to have a quite high diversity of seagrass. Information on the diversity of seagrass species is important due to the important value of seagrass in the marine ecosystem. This research aimed to analyze biodiversity and phylogenetic of seagrass from Sekotong, West Lombok, Indonesia using DNA barcoding of rbcL gene. As many as 35 samples from seven morphologically identified species (Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halodule pinifolia, Halophila ovalis, and H. minor) were taken from four Gilis (small island) in Sekotong. The DNA was amplified for the rbcL gene and sequence analyses using BLAST were conducted to determine the species. Phylogenetic analyses were carried out using three evolutionary algorithms using Neighbor-Joining, Maximum Likelihood and Bayesian analysis with 1000 bootstrap. The rbcL gene was successfully amplified from all samples with a maximum length of 552 bp. The phylogenetic analysis showed that clades were split by family and genera where six clades were formed (Enhalus acoroides, T. hemprichii, Halophila complex, H. pinifolia, S. isoetifolium, and C. rotundata) with more than 95% of bootstrap values for Neighbor-Joining and Bayesian. The p-distance values between species were 0.008-0.097 and the polymorphic site was not found within species. The rbcL sequences only confirmed five seagrass species out of seven morphologically identified species and the sequences generated from this study cannot discriminate Halophila ovalis and H. minor.

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Section: Introductionmentioning

confidence: 99%

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Stevanus

Pharmawati

2020

Biodiversitas

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“…The sequence results obtained were then analyzed using the Mega 6.0 program (Molecular Evolutionary Genetic Analysis) for the alignment process and phylogenetic analysis [17], [18]. The sequence results were then identified in the GenBank service at NCBI (National Center for Biotechnology Information) using the BLAST (Basic Local Alignment Search Tool) menu [19] and compared with other hammerhead sharks found in GenBank.…”

Section: Data Analysesmentioning

confidence: 99%

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Bahri

Hikmah²,

Fadli³

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Hammerhead sharks are particularly vulnerable to fishing because of their slow growth rate and reproductive constraints. This type of sharks needs special attention due to its conservation status. This research was aimed to describe the relationships of the hammerhead sharks from West Aceh waters with the hammerhead sharks from other places. Samples of the shark were collected from Ujong Baroh Fish Port, West Aceh. The samples were analysed at the Laboratory of Department of Marine Science and Fisheries, Syiah Kuala University. The laboratory activity was started with extraction of DNA enhanced by Polymerase Chain Reaction method and visualized by an electrolytical. Analysis using BLAST showed that the first sample obtained 94%-95% cover query value and produced a 683 base pair length while the second sample obtained 98% per sample for a cover query value and produced a 658 base pair length. The average nucleotide values of the hammerhead shark were T=32.8, C=26.6, A=25.7 and G=14.9. The phylogenetic tree shows that the hammerhead sharks from the study area were more similar to the hammerhead sharks from Australia and Malaysia. Overall, based on the nucleotide composition, phylogenetic tree and genetic distance, the hammerhead sharks from the study area are unique compared to the populations from the other places.

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Genetic diversity information of seagrass Enhalus acoroides and Cymodocea rotundata for the local genetic conservation at North Maluku

Cited by 2 publications

References 20 publications

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

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