Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens)

Abstract

Although based on very limited M and L segment sequences, Artybash virus (ARTV) was proposed previously as a unique hantavirus harbored by the Laxmann's shrew (Sorex caecutiens). To verify this conjecture, lung tissues from 68 Laxmann's shrews, captured during 2006 to 2014 in eastern Siberia, Russia, and Hokkaido, Japan, were analyzed for ARTV RNA using reverse transcription polymerase chain reaction (RT-PCR). ARTV RNA was detected in six Laxmann's shrews. Pairwise alignment and comparison of partial-and full-length S, M, and L segment sequences from these Laxmann's shrews, as well as phylogenetic analyses, using maximum likelihood and Bayesian methods indicated that ARTV was distinct from other soricine shrew-borne hantaviruses and representative hantaviruses harbored by rodents, moles, and bats. Taxonomic identity of the ARTVinfected Laxmann's shrews was confirmed by full-length cytochrome b mitochondrial DNA sequence analysis. Our data indicate that the hantavirus previously known as Amga virus (MGAV) represents genetic variants of ARTV. Thus, the previously proposed designation of ARTV/MGAV should be replaced by ARTV.

Genetic Diversity and Phylogeography of Thottapalayam thottimvirus (Hantaviridae) in Asian House Shrew (Suncus murinus) in Eurasia

“…Total RNA was extracted from RNAlater R -preserved lung tissues, using the GC series Magtration R -MagaZorb R RNA Common N Kit or MagDEA RNA 100 Kit (Precision System Science, Matsudo, Japan), and then reverse transcribed, using PrimeScript TM II 1st strand cDNA Synthesis Kit (Takara Bio, Otsu, Japan) and oligonucleotide primer (OSM55F, 5 ′ -TAGTAGTAGACTCC−3 ′ ), designed from the conserved 5 ′ends of the S, M, and L segments of hantaviruses (Klempa et al, 2006(Klempa et al, , 2007Song et al, 2007c;Arai et al, 2008Arai et al, , 2016a.…”

Integrative taxonomy of theRhinolophus macrotiscomplex (Chiroptera, Rhinolophidae) in Vietnam and nearby regions

“…Two mitochondrial genes were sequenced in three laboratories (the Centre National de Séquençage, France; the Biological Research Centre of the Hungarian Academy of Sciences, Hungary; and the Infectious Disease Surveillance Center, Japan) for this study: the complete cytochrome b (Cytb; 1,140 bp) and the 5 0 fragment of cytochrome c oxidase subunit I (COI; 657 bp). Primer sets used for PCR amplification of Cytb were Mt-14724F/Cyb-15915R (Irwin, Kocher, & Wilson, 1991), Cy-14726F/Cyb-15909R (Arai et al, 2016), or Molcit-F/Cytb-H (Ib añez, Garc ıa-Mudarra, Ruedi, Stadelmann, & Juste, 2006;Weyeneth, Goodman, Stanley, & Ruedi, 2008) and of COI were UTyr/C1L705 (Hassanin et al, 2012) or VF1d/ VR1d (Ivanova, Zemlak, Hanner, & Hebert, 2007) (Table S3).…”

Đakrông virus, a novel mobatvirus (Hantaviridae) harbored by the Stoliczka’s Asian trident bat (Aselliscus stoliczkanus) in Vietnam

“…Using the MagDEA RNA 100 Kit (Precision System Science, Matsudo, Japan) 39 , total RNA was extracted from lung tissues of 215 bats, representing 15 genera and 46 species in five families (Hipposideridae, Megadermatidae, Pteropodidae, Rhinolophidae and Vespertilionidae) (Supplemental Table 1). RNA was reverse transcribed to cDNA was synthesized using the PrimeScript II 1st strand cDNA Synthesis Kit (Takara bio, Otsu, Japan) and an oligonucleotide primer (OSM55F, 5′–TAGTAGTAGACTCC–3′) designed from conserved 5′–end of the S, M and L segments of hantaviruses 39 .…”

“…Oligonucleotide primers previously used to detect hantaviruses 12,13,16,39–43 were employed to amplify S, M and L segments (Supplemental Table 2). First-round PCR was performed in 20-μL reaction mixtures, containing 250 μM dNTP, 2.5 mM MgCl 2 , 1 U of Takara LA Taq polymerase Host Start version (Takara Bio) and 0.25 μM of each primer 16 .…”