Genetic diversity of Mycobacterium tuberculosis isolates from the central, eastern and southeastern Ethiopia

Agonafir, Mulualem; Belay, Gurja; Maningi, Nontuthuko E.; Feleke, Adey; Reta, Melese Abate; Olifant, Sharon L.; Hassen, Mohammed Suaudi; Girma, Tewodros; Fourie, P. Bernard

doi:10.1016/j.heliyon.2023.e22898

Cited by 3 publications

(2 citation statements)

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“…TB is the leading HIVassociated opportunistic infection and the main cause of death globally, particularly in resource-limited settings [3]. Globally, HIV-associated TB was estimated to be responsible for about 6.3% of all new cases and 12.8% of all TB-related deaths [1]. Encouragingly, there has been a gradual decline in the prevalence of HIV among new TB cases in recent years [1].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is important to evaluate the genetic diversity of MTb strains to identify genotypes that affect HIV-infected patients. Indeed, determining the genetic diversity of MTb strains has emerged as a crucial public health tool, aiding researchers and TB control programs in comprehending the emergence and spread of specific strains, and assessing the overall impact of genetic diversity on the progression of TB infection and disease [6]. Furthermore, strains of different genetic lineages of MTb demonstrate variability in some biological properties such as the in vitro growth rate, virulence in animal models, and the capacity to acquire drug resistance [7].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Mycobacterium tuberculosis Strains Isolated from HIV-Infected Patients in Mexico

Valencia-Trujillo,

Avila-Trejo,

García-Reyes

et al. 2024

Pathogens

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There has been very limited investigation regarding the genetic diversity of Mycobacterium tuberculosis (MTb) strains isolated from human immunodeficiency virus (HIV)-infected patients in Mexico. In this study, we isolated 93 MTb strains from pulmonary and extrapulmonary samples of HIV-infected patients treated in a public hospital in Mexico City to evaluate the genetic diversity using spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) typing (based on 24 loci). The cohort comprised 80 male and 13 female individuals. There was a positive correlation between a high HIV viral load (>100,000 copies) and extrapulmonary tuberculosis (TB) (r = 0.306, p = 0.008). Lineage 4 was the most frequent lineage (79 strains). In this lineage, we found the H clade (n = 24), including the Haarlem, H3, and H1 families; the T clade (n = 22), including T1 and T2; the X clade (n = 15), including X1 and X3; the LAM clade (n = 14), including LAM1, LAM2, LAM3, LAM6, and LAM9; the S clade (n = 2); Uganda (n = 1); and Ghana (n = 1). We also found 12 strains in the EAI clade belonging to lineage 1, including the EAI2-Manila and EAI5 families. Interestingly, we identified one strain belonging to the Beijing family, which is part of lineage 2. One strain could not be identified. This study reports high genetic diversity among MTb strains, highlighting the need for a molecular epidemiological surveillance system that can help to monitor the spread of these strains, leading to more appropriate measures for TB control in HIV-infected patients.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%