Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers

Ganopoulos, Ioannis; Kazantzis, Konstantinos; Chatzicharisis, Ioannis; Karayiannis, I.; Tsaftaris, Athanasios

doi:10.1007/s10681-011-0416-z

Cited by 96 publications

(55 citation statements)

References 48 publications

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“…In a similar study, association analysis of SSR and ISSR markers with fruit characteristics in sweet cherry cultivars revealed 14 SSR alleles negatively or positively correlated with different morphological traits by using MRA (Ganopoulos et al, 2011). In addition, three ISSR markers were identified to be correlated with fruit harvest time and soluble solids and four ISSR markers were found to be correlated with fruit skin color.…”

Section: Discussionmentioning

confidence: 75%

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

et al. 2015

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ABSTRACT. The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with α-tocopherol and γ-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization.

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Section: Discussionmentioning

confidence: 75%

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

et al. 2015

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“…The most important traits that were included in our developed database were identified by the PCA analysis, which, in this case, allows the multivariate statistical analysis of a large number of variables (Ganopoulos et al, 2015;Ganopoulos et al, 2011). The initial variables were transformed to a decreased number of uncorrelated ones in order to reduce the data, allowing relationships between the individuals and variables to be sufficiently revealed.…”

Section: Multivariate Analysis Of Qualitative Variables: Pca and Ahc mentioning

confidence: 99%

“…In turn, germplasm characterization facilitates the identification of important phenotypic traits. These traits are commonly linked to genetic data and their use is crucial for breeding programs (Ganopoulos et al, 2015;Ganopoulos et al, 2011). Therefore, the clustering analysis and PCA are key tools to group and evaluate cultivars preserved in GeneBank collections (Iezzoni and Pritts, 1991).…”

Section: Multivariate Analysis Of Qualitative Variables: Pca and Ahc mentioning

confidence: 99%

Phenotypic and molecular characterization of apple (Malus × domestica Borkh) genetic resources in Greece

Ganopoulos¹,

Tourvas

Xanthopoulou

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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ABSTRACT:The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward's agglomeration method were used in an unsupervised hierarchical cluster analysis of all cultivars. The cultivars were grouped into four main clusters, suggesting that the characterized apple collection has a high potential for specific breeding goals. Furthermore, the cultivars were genotyped using seven microsatellite primers. Moderate levels of polymorphism were detected, and 38 distinctive alleles (5.4 alleles per primer pair) were identified. Both multivariate clustering approach (phenotypic data) and the genetic distance clustering approach (genetic data) grouped the apple cultivars according to their type. Hence, these data could be used for protection or patenting processes of existing or new apple cultivars carried out by the EU-Community Plant Variety Office.

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“…The distinctiveness among the cherry accessions in breeding and cultivation is fairly important for scientific as well as economic reasons (Struss et al, 2003;Ganopoulos et al, 2011). Cherry production is carried out in the Central Anatolia and Marmara regions, which are suitable regions due to the long harvest time and abundant sunshine (Asma, 2000).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of East Anatolian traditional plum and cherry accessions using SSR markers

Öz¹,

Vurgun²,

Bakır³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We conducted SSR analyses of 59 accessions, including 29 traditional plum (Prunus domestica), 24 sweet cherry (Prunus avium), and 1 sour cherry (Prunus cerasus) selected from East Anatolian gene sources and 3 plum and 2 cherry reference accessions for molecular characterization and investigation of genetic relationships. Eight SSR loci [1 developed from the apricot (UDAp-404), 4 from the peach (UDP96-010, UDP96-001, UDP96-019, Pchgms1) and 3 from the cherry (UCD-CH13, UCD-CH17, UCD-CH31) genome] for plum accessions and 9 SSR loci [5 developed from the cherry (PS12A02, UCD-CH13, UCD-CH17, UCD-CH31, UCD-CH21), 3 from the peach (Pchgms1, UDP96-001, UDP96-005) and 1 from the plum (CPSCT010) genome] for cherry accessions were used for genetic identification. A total of 66 and 65 alleles were obtained in the genetic analyses of 31 East Anatolian plum and cherry plum and 28 cherry accessions, respectively. The number of alleles revealed by SSR analysis ranged from 4 to 14 alleles per locus, with a mean value of 8.25 in plum accessions, and from 5 to 10 alleles per locus with a mean value of 7.2 in cherry accessions. Only one case of synonym was identified among the cherry accessions, while no case of synonym was observed among the plum accessions. Genomic SSR markers used in discrimination of plum and cherry accessions showed high cross-species transferability in the Prunus genus. Because of their appreciable polymorphism and cross species transferability, the SSR markers that we evaluated in this study will be useful for studies involving fingerprinting of cherry and plum cultivars.

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Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers

Cited by 96 publications

References 48 publications

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

Phenotypic and molecular characterization of apple (Malus × domestica Borkh) genetic resources in Greece

Molecular analysis of East Anatolian traditional plum and cherry accessions using SSR markers

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