Genetic diversity studies for grain iron and zinc content analysis for Elite rice (Oryza sativa L.) genotype by using SSR markers

Zulfiqar, Asma; Naseer, Sumbal; Saleem, Ammara; Ahmed, Sahkil; Sardar, Rehana

doi:10.1016/j.jfca.2022.104816

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…However, the growth traits were much higher for ZQUA44 than for ZQUB15 in all three nutrient levels, indicating that trees with different genotypes may respond differently. This result agrees with prior research on Oryza sativa, Vigna radiata, and Zea may that different individuals have different production under the same nutrient levels [17][18][19][20]. To address this further, we conducted comparative transcriptomic sequencing analyses.…”

Section: Discussionsupporting

confidence: 86%

Comparative Transcriptome Analysis of High- and Low-Growth Genotypes of Eucalyptus urophylla in Response to Long-Term Nitrogen Deficiency

Yang,

Xu,

Pan

et al. 2023

Genes

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Nutrients play important roles in the growth and development of most plant species. However, in perennial trees, the function of nutrients in different genotypes is poorly understood. Three different nutrient levels (low, sufficient, and high nutrient levels) were applied to two contrasting Eucalyptus urophylla cultivars (a high-growth cultivar ZQUA44 and a low-growth cultivar ZQUB15), and growth and expression levels were analyzed. Although the growth traits of both genotypes under nutrient starvation treatment were much lower than under abundant nutrients, tree height, crown width, and biomass of different ZQUA44 tissues were much higher than those of ZQUB15 at all three nutrient levels. Differentially expressed genes (DEGs) clustered into six subclusters based on their expression patterns, and functional annotation showed that the DEGs involved in glutathione metabolism and flavonoid biosynthesis may be responsible for nutrient starvation across different genotypes, while the DEGs involved in carotenoid biosynthesis and starch and sucrose metabolism may have a range of functions in different genotypes. The DEGs encoding the MYB-related family may be responsible for nutrient deficiency in all genotypes, while B3 may have different functions in different genotypes. Our results demonstrate that different genotypes may form different pathways to coordinate plant survival when they face abiotic stresses.

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Section: Discussionsupporting

confidence: 86%

Comparative Transcriptome Analysis of High- and Low-Growth Genotypes of Eucalyptus urophylla in Response to Long-Term Nitrogen Deficiency

Yang,

Xu,

Pan

et al. 2023

Genes

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“…Two fine Basmati varieties were chosen for this study based on their iron content. According to our previous study, two Basmati varieties, Basmati-515 and Basmati-198, have the highest and lowest iron contents of 22.0 and 14.1 ppm, respectively, while PK-386 had 19.0 ppm [28]. KSK-133, a coarse variety with high yields and extra-long grains, was chosen.…”

Section: Experimental Designmentioning

confidence: 99%

Iron Sulfate (FeSO4) Improved Physiological Attributes and Antioxidant Capacity by Reducing Oxidative Stress of Oryza sativa L. Cultivars in Alkaline Soil

et al. 2022

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Rice ranks second among cereals in dietary uses around the world. Rice is deficient in iron (Fe), and these are important micronutrients for infants, men, and women. Fortification of rice with iron would help to minimize nutrient deficiency disorders among humans. The current study aims to introduce nutrient-rich rice. The effects of iron on germination, growth, photosynthetic pigment, antioxidant activity, and reduction of oxidative stress were investigated in four Oryza sativa L. cultivars. O. sativa of four different cultivars (Basmati-515, PK-386, KSK-133, and Basmati-198) were grown under five treatments (100, 200, 300, 400, and 500 mM) of iron sulphate (FeSO4) in soil of pH 7.5, along with control, by using six replicates. The result revealed that Fe treatment significantly affected seed germination percentage, plant growth parameters, biomass, photosynthetic pigments (chl a, chl b, total chlorophyll, and carotenoids), antioxidant enzymatic and non-enzymatic activity, and reduced oxidative stress. The findings also showed that Fe application reduced the oxidative stress including malondialdehyde content and hydrogen peroxide, by increasing the antioxidant enzymatic activity, i.e., catalase, ascorbate peroxidase, superoxide dismutase, peroxidase, glutathione peroxidase, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), and non-enzymatic antioxidant compounds (proline, amino acid, total soluble protein, phenolics, flavonoids, reducing-non-reducing sugar, and carbohydrates) in all cultivars of O. sativa. Furthermore, FeSO4 induced a significant increase in proline, free amino acid, and total carbohydrates in the leaves of all O. sativa cultivars, but Basmati-198 showed the significantly highest content by 169, 88, and 110%, respectively, at concentration of 500 mM. The present research work showed that soil application of FeSO4 improved the seed germination, plant growth, and antioxidants enzymatic and non-enzymatic activity, denatured the ROS (reactive oxygen species) in alkaline soil. In order to understand the underlying mechanisms, long-term field investigations should be carried out at the molecular level to examine patterns of iron uptake and plant growth.

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“…Seeds of rice ( Oryza sativa L.) were received from the Rice Research Institute, KSK, and the rice varieties used in the present research work were “Basmati-198, Basmati-515, PK-386, and KSK-133”. Two Basmati varieties were selected in present research work According to our earlier research, the iron concentrations of Basmati-515 and Basmati-198, are 22.0 and 14.1 ppm, respectively, whereas PK-386 has 19.0 ppm [ 23 ]. Seeds of four rice cultivars surface sterilized by using 0.1% bleaching powder (10 to 20 min), then gently rinsed with deionized water before being planted in plastic pots in a natural environment condition (day temperature: 36 o C and night temperature: 27 o C).…”

Section: Methodsmentioning

confidence: 99%

Alkaline and acidic soil constraints on iron accumulation by Rice cultivars in relation to several physio-biochemical parameters

Saleem,

Zulfiqar,

Saleem

et al. 2023

BMC Plant Biol

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Agricultural production is severely limited by an iron deficiency. Alkaline soils increase iron deficiency in rice crops, consequently leading to nutrient deficiencies in humans. Adding iron to rice enhances both its elemental composition and the nutritional value it offers humans through the food chain. The purpose of the current pot experiment was to investigate the impact of Fe treatment in alkaline (pH 7.5) and acidic (pH 5.5) soils to introduce iron-rich rice. Iron was applied to the plants in the soil in the form of an aqueous solution of FeSO4 with five different concentrations (100, 200, 300, 400, and 500 mM). The results obtained from the current study demonstrated a significant increase in Fe content in Oryza sativa with the application of iron in both alkaline and acidic pH soils. Specifically, Basmati-515, one of the rice cultivars tested, exhibited a notable 13% increase in iron total accumulation per plant and an 11% increase in root-to-shoot ratio in acidic soil. In contrast to Basmati-198, which demonstrated maximum response in alkaline soil, Basmati-515 exhibited notable increases in all parameters, including a 31% increase in dry weight, 16% increase in total chlorophyll content, an 11% increase in CAT (catalase) activity, 7% increase in APX (ascorbate peroxidase) activity, 26% increase in POD (peroxidase) activity, and a remarkable 92% increase in SOD (superoxide dismutase) in acidic soil. In alkaline soil, Basmati-198 exhibited respective decreases of 40% and 39% in MDA and H2O2 content, whereas Basmati-515 demonstrated a more significant decrease of 50% and 67% in MDA and H2O2 in acidic soil. These results emphasize the potential for targeted soil management strategies to improve iron nutrition and address iron deficiency in agricultural systems. By considering soil conditions, it is possible to enhance iron content and promote its availability in alkaline and acidic soils, ultimately contributing to improved crop nutrition and human health.

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Genetic diversity studies for grain iron and zinc content analysis for Elite rice (Oryza sativa L.) genotype by using SSR markers

Cited by 8 publications

References 22 publications

Comparative Transcriptome Analysis of High- and Low-Growth Genotypes of Eucalyptus urophylla in Response to Long-Term Nitrogen Deficiency

Comparative Transcriptome Analysis of High- and Low-Growth Genotypes of Eucalyptus urophylla in Response to Long-Term Nitrogen Deficiency

Iron Sulfate (FeSO4) Improved Physiological Attributes and Antioxidant Capacity by Reducing Oxidative Stress of Oryza sativa L. Cultivars in Alkaline Soil

Alkaline and acidic soil constraints on iron accumulation by Rice cultivars in relation to several physio-biochemical parameters

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