Genetic Diversity Studies in Cucurbits Using Molecular Tools

Leiva-Brondo, Miguel; Nuez, F.; Picó, Belén; Wang, Yihong; Behera, T. K.; Kole, Chittaranjan

doi:10.1201/b11436-6

Cited by 21 publications

(25 citation statements)

References 201 publications

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“…The firts coordinate, explaining 10% of the variation, separated all the commercial cultivars of the Zucchini Group from the other commercial groups and from the Spanish landraces, supporting the differentiation of this modern group. Paris et al (2003) and previous studies performed with first generation molecular markers (Esteras et al, 2011), also found that the Zucchini group, the most recent of the subspecies, is the most distinct of the edible fruited cultivargroups of ssp. pepo.…”

Section: Relationships Among Varietiesmentioning

confidence: 85%

“…The genetic variability within C. pepo has been previously assesed using allozymes and different DNA marker systems (Restriction Fragment Length Polymorphism, RFLP, Random Amplified Polymorphic DNA, Amplified Fragment Lenght Polymorphism, AFLP, and Inter Simple Sequence Repeats, ISSRs) (reviewed in Lebeda et al 2007 andin Esteras et al 2011). Most of the studies have been focused on the assessment of the genetic and evolutionary relationships between the wild types and the domesticates, between the two subespecies, and among the cultivar groups, including only a few representatives of European landraces (Ferriol et al 2003;Paris et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Spanish Cucurbita pepo landraces: an unexploited resource for summer squash breeding

Formisano

Roig

Esteras

et al. 2011

Genet Resour Crop Evol

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Cucurbita pepo is a world-wide cultivated vegetable of American origin. Most of the widely grown comercial types are known as Summer squashes and belong to the elongated forms of C. pepo ssp. pepo (Cocozelle, Vegetable marrow and Zucchini Groups). These forms were developed in Europe after the arrival of the first american landraces through a process of selection and fixation that led to the loss of genetic diversity. Part of the genetic variability included on the first American cultigens remains in diverse landraces, still cultivated for self-consumption and sale in local markets. Using the first colecction of genomic and EST derived microsatellites that has just become available for the species, we compared the natural variation present in a collection of Spanish landraces with that of a set of commercial varieties and hybrids, representing the current Summer squash market offer. A total of 194 alleles allowed us to distinguish all genotypes, even those closely related. In general, Cocozelle and Vegetable marrow, Groups with considerably long histories, were more variable than the Zucchini Group, of more recent origin. We found significant genetic diversity among landraces. Variation present in the local accessions belonging to the Zucchini Group was larger than that of commercial cultivars. Cluster, principal coordinates and population structure results suggested that the variation of the Spanish landraces has not been extensivelly used in breeding. Commercial Summer squashes can then benefit from this underexploited variability, specially from some landraces that already displayed favourable commercial traits.

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Section: Relationships Among Varietiesmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Spanish Cucurbita pepo landraces: an unexploited resource for summer squash breeding

Formisano

Roig

Esteras

et al. 2011

Genet Resour Crop Evol

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“…In total, 22 Cucurbita accessions from Angola were assayed, along with 32 Cucurbita spp controls from a wide range of origins, representing most of the cultivated and wild species of the genus. 4 Seeds of the control accessions were supplied by the United States Department of Agriculture (USDA), the Centro Agronómico Tropical de Investigación y Enseñanza (CATIE), the COMAV and the Vavilov Institute of Plant Industry (VIR) genebanks (Tables 1 and 2). …”

Section: Plant Materialsmentioning

confidence: 99%

Angolan vegetable crops have unique genotypes of potential value for future breeding programmes

et al. 2016

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HOW TO CITE:A survey was carried out in Angola with the aim of collecting vegetable crops. Collecting expeditions were conducted in Kwanza-Sul, Benguela, Huíla and Namibe Provinces and a total of 80 accessions belonging to 22 species was collected from farmers and local markets. Species belonging to the Solanaceae (37 accessions) and Cucurbitaceae (36 accessions) families were the most frequently found with pepper and eggplant being the predominant solanaceous crops collected. Peppers were sold in local markets as a mixture of different types, even different species: Capsicum chinense, C. baccatum, C. frutescens and C. pubescens. Most of the eggplant accessions collected belonged to Solanum aethiopicum L. Gilo Group, the so-called 'scarlet eggplant'. Cucurbita genus was better represented than the other cucurbit crops. A high morphological variation was present in the Cucurbita maxima and C. moschata accessions. A set of 22 Cucurbita accessions from Angola, along with 32 Cucurbita controls from a wide range of origins, was cultivated in Valencia, Spain and characterised based on morphology and molecularity using a set of 15 microsatellite markers. A strong dependence on latitude was found in most of the accessions and as a result, many accessions did not set fruit. The molecular analysis showed high molecular variability and uniqueness in the collected accessions, as shown by their segregation from the set of global controls. In summary, the material collected is quite valuable because of its uniqueness and the potential of the breeding characteristics it possesses.

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“…El format de la Tesi basat en articles ja publicats pel grup confirma l´interés dels resultats obtinguts. Esteras et al, 2012b).…”

Section: Resumunclassified

“…Previamente al desarrollo de esta colección, se trataba de poner a punto en Cucurbita SSRs desarrollados en el género Cucumis (melón o pepino). Sin embargo, la tasa de transferibilidad tanto en gSSRs (genómicos) como en EST-SSRs resultaba muy baja (revisado en Esteras et al, 2012b). En este sentido, la doctoranda colaboró en diversos proyectos INIA "Desarrollo de una colección nuclear de germoplasma español de calabacín (C. pepo) y especies relacionadas" (RF2004-00003-00-00) y "Control de la erosión genética en bancos de germoplasma: el caso de la colección de germoplasma español de calabazas y calabacines del COMAV" (RF03-003) transfiriendo marcadores SSRs de Cucumis a Cucurbita (Picó et al, 2005(Picó et al, -2006Fernández-Silva et al, 2008, trabajos incluidos en los anexos 4 y 5) o aplicando marcadores dominantes y menos reproducibles como AFLPs (Amplified Fragment Length Polymorphisms) (Esteras et al, 2009c, trabajo incluido en el anexo 6).…”

Section: Calabacín (C Pepo)unclassified

Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades.

Gómez¹

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Melon (Cucumis melo) and summer squash (Cucurbita pepo) are economically important cucurbits cultivated worldwide. New, better-adapted, cultivars, resistant to pests and diseases, and varieties displaying better quality traits are required to optimize their production and to meet market demands. Crop breeding must be carried out efficiently and competitively, using the existing genetic knowledge and the molecular tools available for each species. The development of genomic tools to speed up Cucurbits breeding programs is the main objective of this Doctoral Thesis.Molecular markers are essential tools for the construction of genetic maps, for an efficient MAS selection, for QTL (Quantitative Trait Loci) analysis and mapping and for the development of new breeding populations. Molecular markers are also necessary tools for biodiversity analysis. High-quality markers, such as microsatellites (Simple Sequence Repeats, SSRs) and SNPs (Single Nucleotide Polymorphisms) have been developed and/or validated for both species in this Thesis. The generation of sequence information, required for the development of SSRs and SNPs has dramatically changed in the last 6 years due to the advent of new technological advances. In fact, a fairly complete melon transcriptome has been produced using high-throughput 454 sequencing (NGS, Next Generation Sequencing) and combining the newly produced ESTs with the previously existing Sanger sequences. The melon transcriptome characterization presented is the most complete to date (53.252 unigenes with over 63% of them annotated). The generation of large SSRs and SNPs collections for these species, by mapping the ESTs (Expressed Sequence Tags) against the new version of the transcriptome has been a great achievement starting a new era in melon breeding.The obtained collection of molecular markers (more than 3.000 SSRs and 38.000 SNPs) has allowed the construction of melon maps with increased marker density. 6Similar resources have been developed for the first time in C. pepo. SSRs identified in the C. pepo transcriptome sequencing project has been applied to localize landraces with favourable commercial traits and whose alleles might be exploited in breeding programs. Likewise, the usefulness of both EST-SSRs and genomic SSRs for depicting genetic relationships has been proved. On the other hand, the employment of the first SNP set identified for Cucurbita through a massive genotyping platform, GoldenGate, has been a success (90%). The genetic map obtained, with more than 300 SNPs, has allowed to map a large set of QTLs related to early flowering, femaleness tendency, shape, size and fruit color, and other traits of interest in breeding.Therefore, these works report the validation and application of previously developed markers and the generation of new genomic tools to speed up the breeding process of these crops. The paper-based format of this Thesis confirms the interest of the obtained results. 7 RESUMENEl melón (Cucumis melo) y el calabacín (Cucurbita pepo) son especies cucurbi...

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Genetic Diversity Studies in Cucurbits Using Molecular Tools

Cited by 21 publications

References 201 publications

Genetic diversity of Spanish Cucurbita pepo landraces: an unexploited resource for summer squash breeding

Genetic diversity of Spanish Cucurbita pepo landraces: an unexploited resource for summer squash breeding

Angolan vegetable crops have unique genotypes of potential value for future breeding programmes

Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades.

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