Genetic evidence that the outer membrane protein YOP1 of Yersinia enterocolitica mediates adherence and phagocytosis resistance to human epithelial cells

Heesemann, J.

doi:10.1016/0378-1097(87)90512-x

Cited by 66 publications

(95 citation statements)

References 6 publications

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“…1B). In contrast, YadA-mediated cell entry of E. coli was hardly detectable (Ͻ0.1%), which is in full agreement with previous reports on YadA-mediated cell entry (5,22,57). When both adhesive factors were expressed simultaneously, neither the adhesion nor the invasion efficiency of the invasinmediated uptake process was significantly reduced (Fig.…”

Section: Yada Expression Does Not Interfere With Invasin Functionsupporting

confidence: 92%

“…Of these factors, invasin also allowed efficient uptake of yersiniae into mammalian cells (26,43). In contrast, the YadA protein promoted significant attachment to eukaryotic cells but its contribution to bacterial entry, relative to that of invasin, seemed small (5,22,57). Experiments in this study demonstrated that YadA of Y. pseudotuberculosis is an adhesin that has the capacity to mediate very efficient high-level uptake into epithelial cells.…”

Section: Discussionmentioning

confidence: 93%

“…In contrast to YadA of Y. pseudotuberculosis, expression of yadA of Y. enterocolitica did not induce detectable uptake of adherent bacteria into HEp-2 cells; it reduced bacterial entry, most likely mediated by invasin (22). The reason for this difference is not clear, but it is possible that the YadA protein of Y. enterocolitica lacks determinants that are important for the ability of YadA of Y. pseudotuberculosis to promote cellular entry.…”

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confidence: 84%

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The YadA Protein ofYersinia pseudotuberculosisMediates High-Efficiency Uptake into Human Cells under Environmental Conditions in Which Invasin Is Repressed

2002

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The YadA protein is a major adhesin of Yersinia pseudotuberculosis that promotes tight adhesion to mammalian cells by binding to extracellular matrix proteins. In this study, we first addressed the possibility of competitive interference of YadA and the major invasive factor invasin and found that expression of YadA in the presence of invasin affected neither the export nor the function of invasin in the outer membrane. Furthermore, expression of YadA promoted both bacterial adhesion and high-efficiency invasion entirely independently of invasin. Antibodies against fibronectin and ␤ 1 integrins blocked invasion, indicating that invasion occurs via extracellular-matrix-dependent bridging between YadA and the host cell ␤ 1 integrin receptors. Inhibitor studies also demonstrated that tyrosine and Ser/Thr kinases, as well as phosphatidylinositol 3-kinase, are involved in the uptake process. Further expression studies revealed that yadA is regulated in response to several environmental parameters, including temperature, ion and nutrient concentrations, and the bacterial growth phase. In complex medium, YadA production was generally repressed but could be induced by addition of Mg 2؉ . Maximal expression of yadA was obtained in exponential-phase cells grown in minimal medium at 37°C, conditions under which the invasin gene is repressed. These results suggest that YadA of Y. pseudotuberculosis constitutes another independent high-level uptake pathway that might complement other cell entry mechanisms (e.g., invasin) at certain sites or stages during the infection process.

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Section: Yada Expression Does Not Interfere With Invasin Functionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 84%

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The YadA Protein ofYersinia pseudotuberculosisMediates High-Efficiency Uptake into Human Cells under Environmental Conditions in Which Invasin Is Repressed

2002

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“…Known functions are binding to the eukaryotic cell surface and extracellular matrix (ECM) proteins (3,5,15,20,(27)(28)(29)(30). The binding domains mediating adherence are probably located in nonconserved regions of the N terminus of Oca family members.…”

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confidence: 99%

Molecular Analysis of Transport and Oligomerization of the Yersinia enterocolitica Adhesin YadA

et al. 2003

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“…YadA has been shown to mediate (i) autoagglutination (3,29), (ii) mannose-resistant guinea pig hemagglutination (16), (iii) binding of bacteria onto epithelial cells (8,15,24), (iv) binding of bacteria to soluble collagen (11), (v) binding of bacteria to immobilized fibronectin (32), (vi) resistance to normal serum (3,18,28), (vii) binding of bacteria to intestinal brush border vesicles (21), (viii) binding to mucin (22), and (ix) colonization of mouse intestines (17).…”

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confidence: 99%

LcrF is the temperature-regulated activator of the yadA gene of Yersinia enterocolitica and Yersinia pseudotuberculosis

Skurnik

Toivanen

1992

J Bacteriol

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The virulence plasmid of human pathogenic Yersinia species, pYV, encodes secreted proteins, Yop proteins, and an outer membrane protein, YadA. YadA has been associated with binding to a variety of substrates and with interference with host defense. YadA is regulated by temperature and is expressed only at 37 degrees C. Unlike the yop regulon, the yadA gene is not under Ca2+ regulation. Here, we show that LcrF (VirF), the temperature-regulated activator of the yop regulon, also acts as an activator for yadA.

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Genetic evidence that the outer membrane protein YOP1 of Yersinia enterocolitica mediates adherence and phagocytosis resistance to human epithelial cells

Cited by 66 publications

References 6 publications

The YadA Protein ofYersinia pseudotuberculosisMediates High-Efficiency Uptake into Human Cells under Environmental Conditions in Which Invasin Is Repressed

The YadA Protein ofYersinia pseudotuberculosisMediates High-Efficiency Uptake into Human Cells under Environmental Conditions in Which Invasin Is Repressed

Molecular Analysis of Transport and Oligomerization of the Yersinia enterocolitica Adhesin YadA

LcrF is the temperature-regulated activator of the yadA gene of Yersinia enterocolitica and Yersinia pseudotuberculosis

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