Genetic Identity between Bhadawari and Murrah Breeds of Indian Buffaloes (Bubalus bubalis) Using RAPD-PCR

Saifi, H. W.; Bhushan, Bharat; Kumar, Sanjeev; Kumar, Pushpendra; Patra, Biswanath; Sharma, Arjava

doi:10.5713/ajas.2004.603

Cited by 15 publications

(10 citation statements)

References 11 publications

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“…The number of fragments amplified in four breeds ranged from 2 to 10 fragments per primer. The number of DNA fragments amplified with each primer found in the present study is in agreement with several investigators (Chen et al, 2001;Rehman, 2002;Appa Rao et al, 1996;Ali, 2003;Kumar et al, 2003;Saifi et al, 2004;Mahfouz, 2008). Kumar et al (2003) used six oligonucleotide primers for RAPD fingerprinting in order to estimate genetic relationship among four different breeds of sheep.…”

Section: Resultssupporting

confidence: 93%

Characterization of four indigenous sheep breeds ofBalochistan, Pakistan by random amplified polymorphic DNAs

Tariq¹,

Bajwa²,

Jawasreh³

et al. 2012

Afr. J. Biotechnol.

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Genetic diversity among four indigenous breeds of sheep namely Mengali, Balochi, Beverigh and Harnai of Balochistan was detected by random amplified polymorphic DNA (RAPD) technique. Nineteen RAPD primers were initially applied and seventeen were chosen for further analysis, based on band pattern quality, reproducibility and presence of bands. An average of 92 RAPD fragments were obtained by using 17 primers and out of the 36 fragments (39.13%) illustrated monomorphism while, 56 bands (60.87%) were polymorphic in all the four sheep breeds. The number of bands amplified in all the sheep breeds ranged from 2 to 10. The highest number of polymorphic loci 40 was observed in the Mengali breed, while the lowest 28 was in Balochi. Further, 33 and 31 polymorphic loci were seen in Beverigh and Harnai breeds, respectively. The overall gene diversity was highest in the Mengali (0.1474) while the lowest in Balochi breed (0.0998). Results of genetic similarities showed closer proximity between Balochi and Beverigh (0.992), Balochi and Harnai (0.992), and between Beverigh and Harnai (0.996). The resemblance was observed between Mengali and Balochi (0.918), between Mengali and Beverigh breeds (0.931) and between Mengali and Harnai breeds (0.925). The high level of genetic similarity between Balochi, Beverigh and Harnai sheep indicated the close relationship that might be due to common habitat. Further the present study highlighted the presence of diversity among and within breeds that can be used in the selection or crossbreeding programs of sheep. The present study suggests that RAPD-PCR can effectively be used to determine the genetic distances among the sheep breeds.

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Section: Resultssupporting

confidence: 93%

Characterization of four indigenous sheep breeds ofBalochistan, Pakistan by random amplified polymorphic DNAs

Tariq¹,

Bajwa²,

Jawasreh³

et al. 2012

Afr. J. Biotechnol.

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“…Saifi et al (2004) utilizaram marcadores RAPD para verificar a identidade genética entre as raças Murrah e Bhadawari.…”

Section: Introductionunclassified

Variabilidade genética em búfalos estimada por marcadores RAPD

Albuquerque

Egito

Marques

et al. 2006

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi caracterizar, por meio de marcadores RAPD, dois grupos genéticos de búfalos, Carabao e tipo Baio, que estão sendo conservados in situ, assim como verificar as relações genéticas entre eles e os outros três grupos genéticos de búfalos existentes no Brasil, Murrah, Jafarabadi e Mediterrâneo, considerados raças comerciais. Foram estudados 48 animais de cada grupo, com exceção dos grupos Murrah e Mediterrâneo, com 47 e 42 animais, respectivamente, compreendendo um total de 233 animais. Os 21 iniciadores polimórficos geraram 98 marcadores. A variabilidade genética entre e dentro dos grupos foi estimada em 26,5 e 73,5%, respectivamente, sugerindo divergência significativa entre os cinco grupos genéticos. Na análise entre pares de grupos, foi verificado que a maior e a menor divergência estavam em torno de 40 e 18%, quando se compararam os grupos Carabao x Mediterrâneo e Murrah x Jafarabadi, respectivamente. Entre os grupos Baio e Murrah, a análise revelou divergência genética de 20,42%, indicando que esses grupos são distintos. Os cinco grupos são geneticamente distintos, o que reforça a necessidade de conservação dos grupos genéti-cos Carabao e Baio, ameaçados de extinção no Brasil.Termos para indexação: Bubalus bubalis, caracterização genética, marcadores moleculares. Genetic variability of buffaloes estimated by RAPD markersAbstract -The objective of this work was to characterize genetically, using RAPD markers, two genetic groups of buffalos, Carabao and Baio, which are being conserved in situ, as well as to verify the genetic relationship among them and the other three genetic groups of buffalos raised in Brazil, considered as commercial breeds: Murrah, Jaffarabadi and Mediterrâneo. Forty eight animals of each group were studied, with the exception of the Murrah and Mediterrâneo, in which 47 and 42 animals, respectively, were sampled, comprising a total of 233 animals. The 21 polymorphic primers produced 98 markers. Genetic variability within and between groups was estimated in 26.5 and 73.5%, respectively, suggesting a significant divergence among groups. On the analysis between pairs of groups, the divergence was about 40 and 18%, respectively, when the Carabao x Mediterranâneo and the Murrah x Jaffarabadi were compared. The estimated genetic divergence between Baio and Murrah groups was 20.42%, indicating that they are distinct groups. The five genetic groups are genetically distinct, indicating the need to conserve the Carabao and Baio, two genetic groups in danger of extinction in Brazil.

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“…The DNA-RAPD assay which is short oligonucleotide primers of arbitrary sequence to amplify genomic DNA by PCR enables the approach for identifying genetic markers (Eman, et al, 2008). RAPD was utilized in breeds genetic studies in most species, including farm animals like cattle , buffalo (Saifi, et al, 2004), goat (Chen, et al, 2001), chicken (Okumus and Kaya, 2005) and sheep (Okumus and Mercan, 2007). The investigation of genetic diversity and similarity among and within breeds is necessary be providing useful genetic information essential for developing affective management plans for the conservation and improvement of these genetic resources.…”

Section: Introductionmentioning

confidence: 99%

Finger Printing of Rahmani, Chios Sheep Breeds and Their Crosses Using Random Amplified Polymorphic Dna (Rapd)

Mohamed

El-Din

Fahmy

et al. 2016

Journal of Agricultural Chemistry and Biotechnology

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RAPD-PCR was performed using ten random primers to identify the genetic diversity among Rahmani, Chios and their crosses. The appearance of bands on gels would reflect the differences between genotypes of the examined animals. The differences would be detected by number and size of present or absent bands with each primer which could be used as positive or negative genetic markers to distinguish between breeds and their crosses. Primers A7, C7 and C1 were used for fingerprinting to identify the Rahmani breed, where they produced bands of 454 and 724 bp, respectively only with Rahmani breed. Presence of band at 410 bp with primer A9 would be used as a genetic marker for Chios breed. The presence of bands 1634, 1105, 223 and 187 bp with primer B17 and also bands 735, 683, 425 and 395 bp with primer A9 could be used as a fingerprinting for cross (½ C ½ R). Presence of bands 173, 132 and 122 bp with primer A5 would be used as a genetic marker for the reciprocal cross (½ R ½ C). The results of molecular DNA of the experimental sheep breeds and their crosses, showed that polymorphism within crosses is higher than polymorphism within the pure breeds of Rahmani and Chios. Fragments generated by primers showed a polymorphism ratio of 10.8 % between Rahmani and Chios and 25 % between crosses (½ C ½ R and ½ R ½ C). Also, the similarity between Rahmani and Chios breeds was 89.1 %, while it was 75 % between crosses. The results asserted that fingerprinting (RAPD-PCR genetic marker) technique would be a useful tool to differentiate sheep breeds and their crosses.

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Genetic Identity between Bhadawari and Murrah Breeds of Indian Buffaloes (Bubalus bubalis) Using RAPD-PCR

Cited by 15 publications

References 11 publications

Characterization of four indigenous sheep breeds ofBalochistan, Pakistan by random amplified polymorphic DNAs

Characterization of four indigenous sheep breeds ofBalochistan, Pakistan by random amplified polymorphic DNAs

Variabilidade genética em búfalos estimada por marcadores RAPD

Finger Printing of Rahmani, Chios Sheep Breeds and Their Crosses Using Random Amplified Polymorphic Dna (Rapd)

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