Genetic interactions between ANLN and KDR are prognostic for breast cancer survival

Dai, Xiaofeng; Chen, Xiao; Hakizimana, Olivier; Man, Yi

doi:10.3892/or.2019.7332

Cited by 22 publications

(26 citation statements)

References 54 publications

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“…25 Surveys such as that conducted by Dai et al show that ANLN down-regulation takes part in the prognostic of breast cancer survival by interacting with KDR. 26,27 ANLN overexpression leads to poor prognosis and poor clinical outcome to anthracycline-based chemotherapy in breast cancer patients. 15 In our study, we documented that the doxorubicin resistance in MDA-MB-231/ADM cells was significantly higher than that in MDA-MB-231 cells.…”

Section: Discussionmentioning

confidence: 99%

<p>ANLN Directly Interacts with RhoA to Promote Doxorubicin Resistance in Breast Cancer Cells</p>

Wang

Xiang

Huang

et al. 2020

CMAR

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Background: Chemotherapy resistance is the leading cause of cancer treatment failure. This research was conducted to explore a potential link between actin-binding protein anillin (ANLN) and doxorubicin resistance in breast cancer. Materials and Methods: We compared ANLN expression and 50% inhibition concentration (IC50) of doxorubicin in human breast cancer cells (MDA-MB-231) and human breast cancer cells with doxorubicin resistance (MDA-MB-231/ADM). Co-immunoprecipitation was used to investigate the interaction between ANLN and RhoA. The cell viability, apoptosis, gene and protein expression were estimated by MTT, flow cytometry, quantitative real-time PCR and western blot. Results: The doxorubicin resistance in MDA-MB-231/ADM cells (IC50 = 19.40 ± 1.16 μg/ mL) was significantly higher than that in MDA-MB-231 cells (IC50 = 1.65 ± 0.23 μg/mL). ANLN was up-regulated in MDA-MB-231/ADM cells compared to MDA-MB-231 cells. Furthermore, ANLN overexpression promoted cell viability and inhibited apoptosis of MDA-MB-231 cells. The gene and protein expression of multidrug resistance (MDR1) and cancer resistance protein (BCRP) were enhanced by ANLN overexpression in MDA-MB-231 cells. ANLN silencing suppressed cell viability and the expression of MDR1 and BCRP and facilitated apoptosis in MDA-MB-231/ADM cells. Moreover, ANLN promoted RhoA activation by interacting with RhoA. ANLN up-regulation enhanced cell viability and the expression of MDR1 and BCRP and decreased apoptosis of MDA-MB-231 cells. The influence conferred by ANLN overexpression was effectively abolished by C3 transferase. Conclusion: This work revealed that ANLN promoted doxorubicin resistance in breast cancer cells by activating RhoA. Thus, our study suggests a novel target for breast cancer treatment.

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Section: Discussionmentioning

confidence: 99%

<p>ANLN Directly Interacts with RhoA to Promote Doxorubicin Resistance in Breast Cancer Cells</p>

Wang

Xiang

Huang

et al. 2020

CMAR

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“…CDK1 was found to interact with ANLN [56,57], and it is indicated that ANLN mobility is directly or indirectly regulated by CDK1 via phosphorylation [58]. The ANLN-actin-binding protein has been identified as being involved in PI3K/PTEN signaling, which is critical in cell life/death control [59]. CITK was proven to be associated with the localization of F-actin and ANLN at the abscission sites [60].…”

Section: Other Binding Partnersmentioning

confidence: 99%

“…KDR: Kinase insert domain receptor (KDR) is a primary vascular endothelial growth factor receptor that encodes a crucial receptor regulating the cancer angiogenesis/metastasis switch [59].…”

Section: Other Binding Partnersmentioning

confidence: 99%

“…ANLN has been identified as an interactor with KDR that encodes a key receptor mediating the cancer angiogenesis/metastasis switch. The observations have suggested that ANLN acts as the intrinsic connection between PI3K/PTEN and KDR signaling, which represents two critical transitions in carcinogenesis [59]. The interaction between ANLN and KDR could act as ANLN and KDR jointly as a prognostic in cancer survival, which could be applied to control triple negative breast cancer [71].…”

Section: Other Binding Partnersmentioning

confidence: 99%

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Role of Anillin in Tumour: From a Prognostic Biomarker to a Novel Target

Tuan

Lee

2020

Cancers

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Anillin (ANLN), an actin-binding protein, reportedly plays a vital role in cell proliferation and migration, particularly in cytokinesis. Although there have been findings pointing to a contribution of ANLN to the development of cancer, the association of ANLN to cancer remains not fully understood. Here, we gather evidence to determine the applicability of ANLN as a prognostic tool for some types of cancer, and the impact that ANLN has on the hallmarks of cancer. We searched academic repositories including PubMed and Google Scholar to find and review studies related to cancer and ANLN. The conclusion is that ANLN could be a potent target for cancer treatment, but the roles ANLN, other than in cytokinesis and its influence on tumour microenvironment remodeling in cancer development, must be further elucidated, and specific ANLN inhibitors should be found.

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“…Anillin has attracted increasing attention as a potential disease-related gene (ORPH:93213, OMIM:616027). Evidence point at anillin expression levels being associated with cell proliferation and migration disorders in cancer and kidney diseases [146][147][148][149] . Additional roles for this actin binding protein have been reported in nerve cell development 150,151 and dysregulation of Anillin has been implicated in central nervous system myelin disorders 152,153 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the zebrafishatoh7−/−mutant,lakritz, highlights Atoh7-dependent genetic networks with potential implications for human eye diseases

Covello

Rossello

Filosi

et al. 2020

Preprint

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Short Running Title: Atoh7 genetic networks and human eye diseases. Nonstandard Abbreviations: RPCs: retinal progenitor cells NCRNA: retinal non-attachment ONH: optic nerve hypoplasia ONA: optic nerve aplasia PHPV: persistent hyperplastic primary vitreous RGCs: retinal ganglion cells EFTFs: eye field transcription factors ABSTRACT: Expression of the bHLH transcription protein Atoh7 is a crucial factor conferring competence to retinal progenitor cells for the development of retinal ganglion cells. A number of studies have emerged establishing ATOH7 as a retinal disease gene. Remarkably, such studies uncovered ATOH7 variants associated with global eye defects including optic nerve hypoplasia, microphthalmia, retinal vascular disorders and glaucoma. The complex genetic networks and cellular decisions arising downstream of atoh7 expression, and how their dysregulation cause development of such disease traits remains unknown. To begin to understand such Atoh7-dependent events in vivo we performed transcriptome analysis of wild type and atoh7 mutant (lakritz) zebrafish embryos at the onset of retinal ganglion cell differentiation. We investigated in silico interplays of atoh7 and other disease-related genes and pathways. By network reconstruction analysis of differentially expressed genes we identified gene clusters enriched in retinal development, cell cycle, chromatin remodelling, stress response and Wnt pathways. By weighted gene coexpression network we identified coexpression modules affected by the mutation and enriched in retina development genes tightly connected to atoh7. We established the groundwork whereby Atoh7-linked cellular and molecular processes can be investigated in the dynamic multitissue environment of the developing normal and diseased vertebrate eye. 3 KEY WORDS: Atoh7, Ath5, retinal ganglion cells, transcriptome analysis; inherited eye diseases; human retina; zebrafish 6 anesthetized for 5-10 min in Ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, Saint Louis, MO, USA) in E3 medium. The corresponding body of each embryo was collected and used immediately to perform genotyping analysis to identify the corresponding to lakritz and wild type eyes. All embryos were collected from the same batches of fish stock to maintain a uniform genetic background. DNA extraction from zebrafish body biopsies and genotyping Genomic DNA extraction from each single body was performed in 100 μl of lysis buffer containing Proteinase K-20mg/ml (EuroClone S.p.A. Milan, Italy), 2 M Tris-HCl ph 8.0, 0.5 M EDTA ph 8.0 and 5 M NaCl, 20 % SDS in a final volume of 50 μl ultra H20. After 3 hours of incubation at 65°C, the gDNA was purified with an Ethanol precipitation step and resuspended in 50 μl of Dnase/Rnase H2O. The genotyping was performed by Restriction Fragment Length Polymorphism (RFLP) assay as previously described 6 . An ∼ 300 bp fragment of atoh7 was PCR amplified with 1 U Taq DNA polymerase (Applied Biosystems by Life Technologies, Carlsbad, CA, USA) according to manufacturer protocols in a 30μl PC...

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Genetic interactions between ANLN and KDR are prognostic for breast cancer survival

Cited by 22 publications

References 54 publications

<p>ANLN Directly Interacts with RhoA to Promote Doxorubicin Resistance in Breast Cancer Cells</p>

<p>ANLN Directly Interacts with RhoA to Promote Doxorubicin Resistance in Breast Cancer Cells</p>

Role of Anillin in Tumour: From a Prognostic Biomarker to a Novel Target

Transcriptome analysis of the zebrafishatoh7−/−mutant,lakritz, highlights Atoh7-dependent genetic networks with potential implications for human eye diseases

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