Genetic Mapping and Molecular Breeding in Cucurbits

Wang, Yihong; Dean, Ralph A.; Joobeur, Tarek

doi:10.1002/9780470650349.ch5

Cited by 6 publications

(7 citation statements)

References 84 publications

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“…The use of high density SNP array screening for a whole population is the most efficient way of mapping QTLs but it is highly expensive. Thus, in order to reduce the cost, we used a small population for screening with a 90K SNP array as described by Wang ( 2014 ), where a population of 40 RILs was employed to verify QTLs with PVEs of 20–30% on specific chromosomes based on the marker density at 5 cM. In the present study, 45 RILs were randomly selected and genotyped using a 90K SNP array and four QTLs were mapped onto chromosomes 2B, 3B, 4B/6A, and 7D.…”

Section: Discussionmentioning

confidence: 99%

QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

Yuan

Zeng

et al. 2018

Front. Plant Sci.

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Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a devastating foliar disease that affects common wheat and barley throughout the world. The reasonable deployment of adult plant resistance (APR) wheat varieties is one of the best methods for controlling this disease. Wheat landraces are valuable resources for identifying the genes/QTLs responsible for disease resistance. Humai 15 is a Chinese spring wheat landrace and it has exhibited adequate levels of APR to the prevalent Pst races in field environments for many years. In this study, a population of 177 recombinant inbred lines (RILs) was derived from Humai 15 × Mingxian 169. After screening based on a 90K chip array using 45 RILs and Kompetitive Allelic Specific PCR marker genotyping for the population of RILs, a major effect QTL in Humai 15 was located on the centromere of chromosome 2B, where it accounted for up to 47.2% of the phenotypic variation. Two other minor QTL genes from Humai 15 were located on chromosome arms 3BS and 4BL. The Yr18 gene was identified on chromosome arm 7DS in Mingxian 169.

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Section: Discussionmentioning

confidence: 99%

QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

Yuan

Zeng

et al. 2018

Front. Plant Sci.

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“…The nutritional compositions of C. sativus include protein, fat and carbohydrate as primary metabolites; along with dietary fibre which is important for the digestive system. C. sativus contains some essential vitamins and anti-oxidants which are effective in human health (Grubben and Denton, 2004;Wang et al, 2007). C. sativus is used for jaundice, bleeding disorders and anuria; while its seeds are highly nourishing (Gogte, 2000).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Agatemor¹,

Nwodo²,

Anosike³

2018

Afr. J. Biotechnol.

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Cucumber (Cucumis sativus L.) is very common, cultivated throughout the world and often eaten as a raw vegetable without cooking. In this study, the phytochemical and proximate compositions of cucumber were investigated. Quantitative phytochemical analysis of the homogenate of C. sativus fruit showed that reducing sugars (574.36 ± 3.88 mg/g) was highest amount when compared to other phytochemicals, alkaloids (2.22 ± 0.96 mg/g) and flavonoids (2.14 ± 0.56 mg/g) were moderately present, while cyanogenic glycoside (0.21 ± 0.13 mg/g) was the lowest in quantity. Proximate analysis showed that C. sativus fruit contained the following-fibre (1.02 ± 0.01%), moisture (94.2 ± 0.08%), protein (3.01 ± 0.07%), lipid (0.55 ± 0.13%), carbohydrate (0.28 ± 0.09%) and ash (0.94 ± 0.24%) contents.

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“…It is a diploid species with twelve chromosomes (2n = 24) (Dane 1991), and has relatively small genome size (4.5–5.0 × 10 8 bp) (Arumuganathan and Earle 1991; Wang et al 2006). Cucumis melo is classified as two subspecies, melo and agrestis (Jeffrey 1980).…”

Section: Introductionmentioning

confidence: 99%

“…Melon ( Cucumis melo L.) is an economically important Cucurbitaceae crop widely produced in temperate and tropical regions (Fernandez-Trujillo et al 2011 ). It is a diploid species with twelve chromosomes (2n = 24) (Dane 1991 ), and has relatively small genome size (4.5–5.0 × 10 8 bp) (Arumuganathan and Earle 1991 ; Wang et al 2006 ). Cucumis melo is classified as two subspecies, melo and agrestis (Jeffrey 1980 ).…”

Section: Introductionmentioning

confidence: 99%

Mapping quantitative trait loci for fruit traits and powdery mildew resistance in melon (Cucumis melo)

Wang

Huang

et al. 2016

Bot Stud

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BackgroundFruit characters affect consumer preferences and the market value of melons is determined by fruit quality. Most fruit quality-related traits are controlled by multiple genes, and are influenced by environmental factors. Furthermore, powdery mildew is another limiting factor in melon production. To develop new melon cultivars with disease resistance and high quality fruits using the molecular marker-assisted breeding strategy, identification of quantitative trait loci for fruit quality and disease resistance is required.ResultsThe F2 populations from the cross of TARI-08874 (Cucumis melo ssp. melo) and ‘Bai-li-gua’ (C. melo ssp. agrestis) were used to map the quantitative trait loci (QTLs) for fruit-related traits and powdery mildew resistance in two trials. All traits were significantly different (P < 0.05) between parents. The generated linkage map consisted of twelve major linkage groups (LGs), spanning 626.1 cM in total, with an average distance of 8.3 cM between flanking markers. Nineteen QTLs were detected for seven melon traits, among which ten QTLs were localized to the same positions as the corresponding QTLs described in other studies. Four of these QTLs were detected in both trials. The results of identified QTLs in this study suggested that fruit size in the tested populations were mainly determined by fruit diameter and flesh thickness. All of the major QTLs for fruit diameter and flesh thickness were identified on LG5 and LG11. Four QTLs identified responsible for netting width of fruit rind were co-localized with the QTLs for netting density, suggesting similar genetic mechanisms affecting these two traits. Additionally, only one major QTL for powdery mildew resistance was detected on LG2, and it was closely linked to a simple sequence repeat (SSR) marker CMBR120 which was identified in a previous study.ConclusionBecause the netting feature is a crucial factor for external appearance of fruits in Asia market, we focus on mining the genetic information of fruit netting. This is the first report of QTL mapping to netting width. Furthermore, new QTLs were identified for netting density (qND4, qND6, and qND7) and netting width (qNW2, qNW4, qNW6, and qNW7) successfully. In addition, novel QTLs for fruit diameter (qFD5), flesh thickness (qFT11) were also detected.

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Genetic Mapping and Molecular Breeding in Cucurbits

Cited by 6 publications

References 84 publications

QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Mapping quantitative trait loci for fruit traits and powdery mildew resistance in melon (Cucumis melo)

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