2024
DOI: 10.1002/cpz1.70034
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Genetic Modification of Mice Using Prime Editing

Amr R. Salem,
Xiaoling Xie,
Susan H. Griffin
et al.

Abstract: Genetically modifying mice traditionally involved complex methods of designing and validating targeting constructs, embryonic stem cell electroporation and selection, blastocyst injection, and breeding chimeras for germline transmission. Such arduous steps were best carried out by specialized gene targeting cores in academia or through expensive commercial vendors. Further, the time from initiation to completion of a project often took at least 1 year and, in some cases, much longer (or never), with no guarant… Show more

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