Genetic parameters for oocyte number and embryo production within a bovine ovum pick-up–in vitro production embryo-production program

Merton, J.S.; Ask, B.; Onkundi, D.C.; Mullaart, E.; Colenbrander, B.; Nielen, M.

doi:10.1016/j.theriogenology.2009.06.003

Cited by 62 publications

(64 citation statements)

References 18 publications

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“…cow (Hasler, 1998;Merton et al, 2009). However, most laboratories currently culture up to 20 embryos in a 20-50-l droplet or up to 50 embryos in 400-500 l in a well in vitro (Krisher & Wheeler, 2010;Vajta, 2010) to obtain transferrable embryos.…”

Section: Introductionmentioning

confidence: 99%

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

Kang

Ofuji

Imai

et al. 2014

Zygote

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SummaryThe aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.

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Section: Introductionmentioning

confidence: 99%

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

Kang

Ofuji

Imai

et al. 2014

Zygote

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“…The reasons for this variability, which resemble those found in reported superovulatory responses (Sales et al, 2008) are not clear, but should, in part, be explained by genetics. It has been shown that high antral follicle counts may be inherited and are highly repeatable within certain individuals (Merton et al, 2009). It is possible that the OPU sessions in this trial were done too shortly after the rbST administration, and to the same effect, the eCG injection could have been delayed one or two days, so that extra time would be allowed for rbST to act.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, Holsteins usually produce less OPU-derived oocytes in relation to Bos indicus cows. This is also true for embryo production, either after superovulation (Sales et al, 2008) or OPU-derived oocyte culture (Merton et al, 2009).…”

Section: Introductionmentioning

confidence: 98%

Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers

et al. 2012

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-The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimulation (6 Holstein and 8 Tabapuã), 500 mg of rbST (Boostin ® ) on D0 and 500 IU of eCG (Folligon) on D2; and control (6 Holstein and 8 Tabapuã), in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen ® ) and embryos were evaluated seven days after fertilization. There was an effect from the interaction of treatment and breed, so that hormonal stimulation increased antral follicle count (2-8 mm) in Tabapuã (29.9±2.6 to 41.4±2.6), but not in Holstein heifers (14.4±2.6 to 15.5±2.6). Tabapuã heifers had higher mean antral follicle count than Holsteins (35.6±1.8 vs. 15.0±2.1). The number of viable oocytes was not increased by stimulation in Tabapuã (from 4.7±1.0 to 5.2±1.1 in control and simulation, respectively) or in Holstein heifers (1.3±1.9 to 2.0±1.6 in control and simulation, respectively). There was no difference in the percentage of heifers with more than five viable oocytes in the group treated (33 vs 27%). The number of blastocysts was not affected by treatment (1.75 vs. 1.00 in hormonal stimulation and control, respectively). The increase in antral follicle count in the stimulated Tabapuã heifers did not reflect upon oocyte yield. The differential breed response to the hormonal treatment underscores the need for additional tests, especially for the Holstein breed, in order to enhance OPU efficiency.

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“…While the heritability of fertility traits is low (0.05), OPU-IVP related traits (number of cumulusoocyte complexes, quality of cumulus-oocyte complexes, number and proportion of cleaved embryos at day 4, and number and proportion of total and transferable embryos at day 7 of culture) have shown a heritability from 0.10 to 0.25 (Merton et al, 2009). Thus, genetic improvement could be faster for ART traits such as OPU-IVP-ET than for conventional fertility traits in dairy cattle (Kadarmideen et al, 2000).…”

Section: Application Of Quantitative Genetics For Selection Of Embryomentioning

confidence: 99%

Basic and practical aspects of pregnancy establishment in cattle

Pedersen¹,

Mazzoni²,

Stroebech³

et al. 2017

Anim Reprod

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Bovine embryos are increasingly produced using reproductive technologies, e.g. ovum pick-up (OPU), in vitro embryo production (IVP) and embryo transfer (ET). Such in vitro manipulated embryos are known to deviate in several aspects compared to in vivo derived embryos. Pregnancy establishment in cattle involves timed biological events including fine-tuned communication, initiated and carried out by both the embryo and the endometrium. This stimulates research to increase the understanding of events and interactions taking place in the uterus after embryo transfer, both from a biological and systems biology point of view. This review will focus on the biological events taking place during early embryonic development, implantation and beginning of placentation, with focus on transfer of in vitro produced embryos, including a systems biology approach for selection of superior embryo recipients.

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Genetic parameters for oocyte number and embryo production within a bovine ovum pick-up–in vitro production embryo-production program

Cited by 62 publications

References 18 publications

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers

Basic and practical aspects of pregnancy establishment in cattle

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