Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 inPlasmodium falciparumisolates from children in South of Benin

Abstract

The aim of this study was to determine the genetic diversity of Plasmodium falciparum by analyzing the polymorphism of the msp-1 and msp-2 genes and the multiplicity of infection in children with uncomplicated malaria in southern Benin. Blood samples of children with fever or history of fever with thick smear positive P. falciparum were collected on filter paper. After extraction of DNA by Chelex®, the samples underwent nested PCR. 93 isolates from children were genotyped. For the msp-1 gene, the K1 and R033 sequences were the most represented in the study population with 85.2% and 83% prevalence, respectively. Regarding the msp-2 gene, the FC27 family was more highly represented with 99% prevalence against 81.5% for 3D7. Mixed infections accounted for 80.4% of the samples. Twenty-five alleles were identified for msp-1 and 28 for msp-2. Fourteen and ten alleles belonged to the K1 (100–500 bp) and MAD20 (100–500 bp) families, respectively. The RO33 sequence did not show any polymorphism, with only one variant (160 bp) detected. The msp-2 gene was present as 16 FC27 family fragments (250–800 bp) and 12 of the 3D7 family (350–700 bp). The multiplicity of infection was estimated at 3.8 for msp-1 and 3.9 for msp-2 with 77 (87.5%) and 84 (91.3%) samples harboring more than one parasite genotype for msp-1 and msp-2, respectively. The multiplicity of infection (MOI) was influenced neither by age nor by parasite density. This study shows a significant diversity of P. falciparum in southern Benin with an MOI unaffected by age or by parasite density.

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

“…This has also consistent with reports from Congo Brazzaville [20], sub-Saharan Africa [13] and Cameroon [22]. but differsfrom genotyping in Benin [23] and Sudan [24]. This suggests that the diversity of P .…”

Impact of an Irrigation Dam on the Transmission and Diversity of Plasmodium falciparum in a Seasonal Malaria Transmission Area of Northern Ghana

“…is is similar to data reported from other regions of Ghana and countries in sub-Saharan Africa with similar malaria transmission settings [29,[31][32][33]. A study conducted in the south of Benin however reported the FC27 family as the most predominant allelic family [34]. e reported differences in the genetic diversity of P. falciparum infections may be due to host-parasite interactions, transmission intensity, ethnicity, and geographical location.…”

Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

“…We found that of the 72 allelic types detected in msp-1, the K1 allelic family with 20.8% allelic frequency besides its representation in poly allelic bands was predominant. This is in line with a previous study from Kolla-Shele area, Arbaminch Zuria District, southwest Ethiopia [46] and studies in Ghana and Benin [51,52]. However, studies in Northern Ethiopia [50] and Malaysia [53] reported Mad20 allele was predominant.…”

“…However, the FC27 family was more frequently detected 18(26.1%) in our study. This is consistent with reports from studies in Benin [51] and Sudan [58] but differs from previous study in Ethiopia [57] and Brazzaville, Republic of Congo [47]. This suggests that the diversity of P. falciparum infections may differ according to geographic location, transmission intensity and sample population.…”