Genetic polymorphisms of 21 STR loci of Golden<i>e</i>ye™ DNA ID 22NC kit in five ethnic groups of China

Zhang, Jiashuo; Bao, Yun; Tao, Ruiyang; Guo, Fei; Sheng, Xiang; Bian, Yingnan; Liu, Xiling; Zhang, Suhua; Li, Chengtao

doi:10.1080/20961790.2018.1479148

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…To determine the concordance between our new system and other commercially available kits, 20 samples were genotyped using our new system, Huaxia Platinum kit (Applied Biosystems) and Goldeneye DNA ID 22NC Kit (Peoplespot) [24, 25], following the manufacturers’ recommendations. The concordance of STR loci CSF1PO, D1S1656, D2S1338, D2S441, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, Penta E, Penta D, TH01, TPOX, and vWA contained in the new typing system was established by comparison to the profiles obtained with Huaxia Platinum kit.…”

Section: Methodsmentioning

confidence: 99%

Development and validation of a forensic six‐dye multiplex assay with 29 STR loci

Tao

et al. 2021

Electrophoresis

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This paper describes the development and validation of a novel 31‐locus, six‐dye STR multiplex system, which is designed to meet the needs of the rapidly growing Chinese forensic database. This new assay combines 20 extended‐CODIS core loci (D3S1358, D5S818, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D8S1179, D18S51, D16S539, D13S317, FGA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), nine highly polymorphic loci in Chinese Han population (D3S3045, D6S1043, D6S477, D8S1132, D10S1435, D15S659, D19S253, Penta D, and Penta E), and two gender determining markers, amelogenin and Y‐Indel, which could amplify DNA from extracts, as well as direct amplification from substrates. To demonstrate the suitability for forensic applications, this system was validated by precision and accuracy evaluation, concordance tests, case sample tests, sensitivity, species specificity, stability, stutter calculation, and DNA mixtures, according to the guidelines described by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and regulations published by the China Ministry of Public Security. The validation results indicate the robustness and reliability of this new system, and it could be a potentially helpful tool for human identification and paternity testing in the Chinese population, as well as facilitating global forensic DNA data sharing.

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Section: Methodsmentioning

confidence: 99%

Development and validation of a forensic six‐dye multiplex assay with 29 STR loci

Tao

et al. 2021

Electrophoresis

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A newly devised multiplex assay of novel polymorphic non-CODIS STRs as a valuable tool for forensic application

Zhang

Tao

et al. 2020

Forensic Science International: Genetics

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An Interpretation of the Genetic Polymorphism and Population Genetic Background of Ankang Han Population via a Novel InDel Panel

Cui

Jin

Fang

et al. 2021

Forensic Sciences Research

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In this research, genotyping data of 43 InDel loci in 311 Han individuals in Ankang City, Shaanxi Province, China were detected using a self-developed five-dye multiplex amplification panel. The allelic frequencies and forensic parameters of all InDel loci were calculated. The combined power of discrimination and probability of exclusion values were 0.999 999 999 999 999 998 827 39 and 0.999 887 424, respectively, which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population. Moreover, genetic distances, pairwise F ST values, principal component analyses, phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups. Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups.

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Genetic polymorphisms of 21 STR loci of Goldeneye™ DNA ID 22NC kit in five ethnic groups of China

Cited by 3 publications

References 18 publications

Development and validation of a forensic six‐dye multiplex assay with 29 STR loci

Development and validation of a forensic six‐dye multiplex assay with 29 STR loci

A newly devised multiplex assay of novel polymorphic non-CODIS STRs as a valuable tool for forensic application

An Interpretation of the Genetic Polymorphism and Population Genetic Background of Ankang Han Population via a Novel InDel Panel

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