Genetic Regulation of the Function of Antibody-Producing Cells

Biozzi, G; Stiffel, C; Mouton, D; Bouthillier, Y; Decreusefond, C

doi:10.1016/b978-0-12-057550-3.50046-6

Cited by 50 publications

(48 citation statements)

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“…This difference in origin and function of PFC and RFC explains why the interline difference is greater as measured in terms of humoral antibodies or antibody-secreting cells (PFC and plasma RFC) than in terms of total RFC (Tables I and II). It has been, in fact, demonstrated that the selective breeding affects only bone marrowderived antibody-producing ceils, while the potentiality of thymus-derived lymphocytes is not modified (4).…”

Section: Discussionmentioning

confidence: 99%

“…dinitrophenyl hapten, 2 pneumococcal polysaccharide 3 and T4 bacteriophage. 4 The two lines of mice also differ in their ability to synthesize humoral antibody against histocompatibility antigens (4), antibody enhancing tumor growth, s and in the incidence of benzopyrene-induced tumors (9). The difference in immunoresponsiveness of the two lines involves the production of various classes of antibody: 19S, 7S 3'2, 7S 3"1, and reagins (6,8).…”

Section: (From the Institut D'immuno-biologie Inserm And Cnrmentioning

confidence: 99%

“…Antigen recognition constitutes the initial discriminative phase of the response which is followed by the productive phase of antibody synthesis. Genetic control of antigen recognition is generally operated by thymus-derived lymphocytes (T) ~ (1-3) while the phase of antibody synthesis depends on genetic regulation of the life cycle of bone marrow-derived lymphocytes (B) (4). The study of responsiveness to antigens of restricted heterogeneity, such as synthetic polypeptides, has led to the identification of specific immune response genes controlling antigen recognition (5).…”

Section: (From the Institut D'immuno-biologie Inserm And Cnrmentioning

confidence: 99%

See 2 more Smart Citations

Cytodynamics of the Immune Response in Two Lines of Mice Genetically Selected for "High" and "Low" Antibody Synthesis

Biozzi

Stiffel

Mouton

et al. 1972

The Journal of Experimental Medicine

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Two lines of mice have been separated by selective breeding for the character "agglutinin production to heterologous erythrocytes." Around the 18th generation of selection the two lines could be considered as homozygous for the character investigated. This trait is under the control of a group of additive genes. The interline difference in the production of anti-SE agglutinins was verified for the range of antigen doses from subimmunogenic to maximal. After intravenous immunization with an optimal dose of SE, the duration of the exponential rise in serum antibody was 4–5 days in both lines. At this time most of the interline difference in responsiveness is already expressed. A cytodynamic study carried out in terms of plaque-forming cells (PFC) and rosette-forming cells (RFC) in the spleen during the exponential phase showed that the principal interline difference is found in the doubling time of cells engaged in the immune response. More precise cytodynamic analysis made in terms of RFC showed that the doubling time of RFC is 9 hr in high responder and 16 hr in low responder mice. The duration of the exponential rise and the number of target cells stimulated by antigen is the same in both lines. The interline difference at the end of the exponential rise (4 days postimmunization) is larger in terms of serum antibody (30–40-fold) than in terms of PFC or RFC (20- and 11-fold, respectively). A morphological study of RFC in nonimmunized mice showed that about 90% of rosettes were formed by small lymphocytes in both lines. The remainder were medium-sized lymphocytes. At the peak of the cellular response the RFC have differentiated into large lymphocytes, blast cells, and plasma cells. The contribution of plasma cells to RFC is much greater in the high than in the low line. The cytodynamic and morphologic results presented in this article are compatible with the hypothesis that the group of genes segregated in each line during the selective breeding control and regulate the rate of multiplication and differentiation of the antibody-producing cells.

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Section: Discussionmentioning

confidence: 99%

Section: (From the Institut D'immuno-biologie Inserm And Cnrmentioning

confidence: 99%

Section: (From the Institut D'immuno-biologie Inserm And Cnrmentioning

confidence: 99%

See 1 more Smart Citation

Cytodynamics of the Immune Response in Two Lines of Mice Genetically Selected for "High" and "Low" Antibody Synthesis

Biozzi

Stiffel

Mouton

et al. 1972

The Journal of Experimental Medicine

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“…Many experiments, carried out in inbred strains of mice, analyzing the role of candidate genes on Ab responsiveness (principally the H-2 and Igh loci) have also provided evidence for large background effects (1)(2)(3)(4). The production of high (H) and low (L) Ab responder lines of mice by selective breeding for Ab production to heterologous erythrocytes has opened the way to investigate the multigenic control of quantitative Ab responses (5). It was estimated that 10 independently segregating loci endowed with additive effects are responsible for the huge (240-fold) multispecific difference separating H and L Ab titer phenotypes (6).…”

mentioning

confidence: 99%

Toward genetic dissection of high and low antibody responsiveness in Biozzi mice

Puel

Mevel

Bouthillier

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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“…It has recently been established through the use of mixed agglutination (4,5), inhibition of rosette formation (6), immunofluorescence (7), and radioautography (7,8) The anti-b4 (2C74-5) and anti-b5 (88K-3) antisera were obtained by immunization of rabbits of alaKb6b6 genotype with purified immunoglobulin-G prepared from pooled sera of alalb4b4 and alalblb5 rabbits, respectively (12).…”

mentioning

confidence: 99%

Membrane-Associated Immunoglobulin of Rabbit Peripheral Blood Lymphocytes: Allelic Exclusion at the b Locus

Davie

Paul

Mage

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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Light chain allotypic determinants were demonstrated on the surface of peripheral blood lymphocytes of rabbits. The anti-b4 (2C74-5) and anti-b5 (88K-3) antisera were obtained by immunization of rabbits of alaKb6b6 genotype with purified immunoglobulin-G prepared from pooled sera of alalb4b4 and alalblb5 rabbits, respectively (12).Anti-b4 allotype antibodies were specifically purified by first passing the antiserum over an agarose conjugate (13) of b5-IgG to remove any cross-reacting antibodies. The effluent was then allowed to absorb to a b4-IgG-agarose column.The anti-b4 antibodies were then eluted with a glycine -HC1 430 Abbreviation: PBS, phosphate-buffered saline.

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Genetic Regulation of the Function of Antibody-Producing Cells

Cited by 50 publications

References 14 publications

Cytodynamics of the Immune Response in Two Lines of Mice Genetically Selected for "High" and "Low" Antibody Synthesis

Cytodynamics of the Immune Response in Two Lines of Mice Genetically Selected for "High" and "Low" Antibody Synthesis

Toward genetic dissection of high and low antibody responsiveness in Biozzi mice

Membrane-Associated Immunoglobulin of Rabbit Peripheral Blood Lymphocytes: Allelic Exclusion at the b Locus

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