Genetic relationships in different sheep populations of Bangladesh based on microsatellite markers

Iranian livestock diversity is still largely unexplored, in spite of the interest in the populations historically reared in this country located near the Fertile Crescent, a major livestock domestication centre. In this investigation, the genetic diversity and differentiation of 10 Iranian indigenous fat-tailed sheep breeds were investigated using 18 microsatellite markers. Iranian breeds were found to host a high level of diversity. This conclusion is substantiated by the large number of alleles observed across loci (average 13.83, range 7-22) and by the high within-breed expected heterozygosity (average 0.75, range 0.72-0.76). Iranian sheep have a low level of genetic differentiation, as indicated by the analysis of molecular variance, which allocated a very small proportion (1.67%) of total variation to the between-population component, and by the small fixation index (FST = 0.02). Both Bayesian clustering and principal coordinates analysis revealed the absence of a detectable genetic structure. Also, no isolation by distance was observed through comparison of genetic and geographical distances. In spite of high within-breed variation, signatures of inbreeding were detected by the FIS indices, which were positive in all and statistically significant in three breeds. Possible factors explaining the patterns observed, such as considerable gene flow and inbreeding probably due to anthropogenic activities in the light of population management and conservation programmes, are discussed.

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“…) and Bangladeshi (Khan et al . ) populations. Although most of these investigations used either a larger (Peter et al .…”

Section: Discussionmentioning

confidence: 99%

Multilocus genotypic data reveal high genetic diversity and low population genetic structure of Iranian indigenous sheep

et al. 2016

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“…The breed of sampled sheep was indigenous which is predominant all over Bangladesh (Bhuiyan, 2006). However, a study based on micro-satellite markers by Khan et al (2009) described Garole sheep of Satkhira district (within Khulna division) as an independent sheep breed in Bangladesh. So, the prevalence estimated in this study is based on a representative sample of goats and sheep and would therefore be applicable to the goats and sheep (except Khulna division) populations of Bangladesh.…”

Section: Discussionmentioning

confidence: 99%

Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh

Rahman¹,

Saegerman²,

Berkvens³

et al. 2013

Preventive Veterinary Medicine

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a b s t r a c tThe true prevalence of brucellosis and diagnostic test characteristics of three conditionally dependent serological tests were estimated using the Bayesian approach in goats and sheep populations of Bangladesh. Serum samples from a random selection of 636 goats and 1044 sheep were tested in parallel by indirect ELISA (iELISA), Rose Bengal Test (RBT) and Slow Agglutination Test (SAT). The true prevalence of brucellosis in goats and sheep were estimated as 1% (95% credibility interval (CrI): 0.7-1.8) and 1.2% (95% CrI: 0.6-2.2) respectively. The sensitivity of iELISA was 92.9% in goats and 92.0% in sheep with corresponding specificities of 96.5% and 99.5% respectively. The sensitivity and specificity estimates of RBT were 80.2% and 99.6% in goats and 82.8% and 98.3% in sheep. The sensitivity and specificity of SAT were 57.1% and 99.3% in goats and 72.0% and 98.6% in sheep. In this study, three conditionally dependent serological tests for the diagnosis of small ruminant brucellosis in Bangladesh were validated. Considerable conditional dependence between IELISA and RBT and between RBT and SAT was observed among sheep. The influence of the priors on the model fit and estimated parameter values was checked using sensitivity analysis. In multiple test validation, conditional dependence should not be ignored when the tests are in fact conditionally dependent.

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“…For example, Chotonagpuri sheep breed of India is found in Meherpur and Chapainawabganj district and its adjacent regions of Bangladesh. Moreover, Garoleis, one of the promising sheep breed in West Bengal of India is also found in Sundarban area in the southern part of Bangladesh (Sharma et al, 1999;Islam and Shahjalal, 2001;Khan et al, 2009). Indigenous sheep of Bangladesh was crossed in limited scale with (i) three heads of Lohi rams from Pakistan in December 1964, (ii) (Hassan and Talukder, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Phenotypic characteristics of sheep population of Bangladesh were described intensively by research (Hassan and Talukder, 2011;Pervage et al, 2009). However, information on genetic characterization of Bangladeshi sheep populations is scare (Tsunoda et al, 1984;Khan et al, 2009) used isozymes method for characterization of Bangladeshi sheep. Khan et al (2009) (FAO, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Genetic relationship among indigenous sheep population of Bangladesh

Deb

Choudhury

Kabir

et al. 2019

Bang. J. Anim. Sci.

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The study was conducted to investigate the genetic relationships among indigenous sheep population of Bangladesh (Barind, Jamuna river basin, Coastal and Garole sheep) using microsatellite markers. A total of 96 blood samples were collected from adult sheep of Barind (24), Jamuna River Basin (24), Coastal (24), Garole(10) and available Chotanagpuri (10) sheep. Chotanagpuri sheep was used as an outgroup population. DNA was extracted from blood samples using QIAGEN DNA Mini extraction kit and was quantified using a nanodrop. FAO recommended 13 labeled microsatellite markers were used for polymerase chain reaction (PCR). PCR product was confirmed by 2% agarose gel electrophoresis and visualized by staining with ethidium bromide.The exact allele sizes in each primer were determined by GeneMaker V1.85 demo. Microsatellite tool kit and Dispan software package were used for calculation of allele frequency, number of alleles per locus, observed and expected heterozygosity and genetic distances (DA). The Dispan software was used to calculate inter-individual genetic distances. These distance values were used to construct an UPGMA tree. Results showed that average number of polymorphic alleles per locus varied from4 in HUJ616 to 12 in MAF70. Observed heterozygosity was also varied from 0.54±0.04 in Coastal to 0.63±0.03 in Barind sheep population. Genetic distance between Jamuna river basin and Barind was lowest (0.01) and between Garole and Costal was highest (0.17). Garoleand Chotonagpuri sheep has higher genetic distance from other three sheep populations. Phylogenetic dendogram showed that sheep of Jamuna river basin and barind were belonged to same genetic group. Whereas, coastal, garole and Nagpur sheeps were shown higher genetic distances from Jamuna river basin and coastal sheep. Considering findings of this study it may be concluded that the Barind and Jamuna river basin sheep belongs to a similar genetic group while, Garole and Coastal sheep are belonging to two distinct genetic groups.

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Genetic relationships in different sheep populations of Bangladesh based on microsatellite markers

Cited by 9 publications

References 4 publications

Multilocus genotypic data reveal high genetic diversity and low population genetic structure of Iranian indigenous sheep

Multilocus genotypic data reveal high genetic diversity and low population genetic structure of Iranian indigenous sheep

Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh

Genetic relationship among indigenous sheep population of Bangladesh

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