Genetic resources of macroalgae: Development of an efficient method using microsatellite markers in non-model organisms

Mauger, Stéphane; Baud, Aurélien; Le Corguillé, Gildas; Tanguy, Gwenn; Legeay, Erwan; Creis, Emeline; Valero, Myriam; Potin, Philippe; Destombe, Christophe

doi:10.1016/j.algal.2023.103251

Cited by 3 publications

References 59 publications

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Eucheumatoid Seaweed Farming in the Sulu Archipelago, Philippines: Practices, Challenges, and Opportunities for Sustainable Development

Halun,

Burias,

Amil-Jinang

et al. 2024

Preprint

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Eucheumatoid Seaweed Farming in the Sulu Archipelago, Philippines: Practices, Challenges, and Opportunities for Sustainable Development

Halun,

Burias,

Amil-Jinang

et al. 2024

Preprint

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Genetic marker: a genome mapping tool to decode genetic diversity of livestock animals

Panchariya,

Dutta,

Ananya

et al. 2024

Front. Genet.

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Genotyping is the process of determining the genetic makeup of an organism by examining its DNA sequences using various genetic markers. It has been widely used in various fields, such as agriculture, biomedical and conservation research, to study genetic diversity, inheritance, the genetic basis of disease-associated traits, evolution, adaptation, etc., Genotyping markers have evolved immensely and are broadly classified as random markers (RFLP, RAPD, AFLP, etc.) and functional markers (SCoT, CDDP, SRAP, etc.). However, functional markers are very limited in genotype studies, especially in animal science, despite their advantages in overcoming the limitations of random markers, which are directly linked with phenotypic traits, high specificity, and similar logistic requirements. The current review surveyed the available random and functional markers for genotyping applications, focusing on livestock including plant and microbe domains. This review article summarises the application, advantages, and limitations of developed markers and methods for genotyping applications. This review aims to make the reader aware of all available markers, their design principles, and methods, and we discuss the marker inheritance patterns of RLFP and AFLP. The review further outlines the marker selection for particular applications and endorses the application of functional markers in genotyping research.

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Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana

Sweiss,

Hasan,

Odat

2024

J. Microbiol. Biotechnol.

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Chlorella sorokiniana green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of C. sorokiniana have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of C. sorokiniana to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the C. sorokiniana genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of C. sorokiniana , and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of C. sorokiniana .

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Genetic resources of macroalgae: Development of an efficient method using microsatellite markers in non-model organisms

Cited by 3 publications

References 59 publications

Eucheumatoid Seaweed Farming in the Sulu Archipelago, Philippines: Practices, Challenges, and Opportunities for Sustainable Development

Eucheumatoid Seaweed Farming in the Sulu Archipelago, Philippines: Practices, Challenges, and Opportunities for Sustainable Development

Genetic marker: a genome mapping tool to decode genetic diversity of livestock animals

Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana

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