Genetic selection for resistance to mycoplasmal pneumonia of swine (<scp>MPS</scp>) in the <scp>L</scp>andrace line influences the expression of soluble factors in blood after <scp>MPS</scp> vaccine sensitization

Shimazu, Tomoyuki; Borjigin, Liushiqi; Katayama, Yuki; Li, Meihua; Satoh, Takumi; Watanabe, Kouichi; Kitazawa, Haruki; Roh, Sanggun; Aso, Hisashi; Katoh, K.; Suda, Yoshihito; Sakuma, Akiko; Nakajo, Mitsuru; Suzuki, Keiichi

doi:10.1111/asj.12158

Cited by 13 publications

(27 citation statements)

References 36 publications

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“…However, there are no reports on the relationship between Mhp or MPS and IL-17. The present result, in which increased breeding value of IFN-γ concentration corresponded to decreased breeding value of morbid MPS lesions over five generations is supported by the reported IFN-γ response to vaccination with Mhp (Shimazu et al 2014). Therefore, the strong negative genetic correlation between MPS lesions and IL-17 in the present study represents an important finding.…”

Section: Genetic Trendssupporting

confidence: 85%

“…The importance of IL-17 in mycoplasmal infection has been reported only for mice (Sieve et al 2009). Shimazu et al (2014) previously reported that IFN-γ concentration in serum after MPS vaccination was significantly higher in the MPS-selected Landrace line compared with a general, non-selected Landrace line. However, an important role of IL-17 in recruitment of the neutrophilic leukocyte in the biophylaxis response to infection is pointed out (Wu et al 2007).…”

Section: Genetic Trendsmentioning

confidence: 89%

“…Selection traits included ADG, backfat thickness (BF), peripheral blood cortisol concentration and morbid MPS lesions (Kadowaki et al 2012). In addition, when the MPS vaccine was inoculated, the increase cytokines such as IFN-γ and IL-10 of the selected group was higher than that in non-selected Landrace pigs (Shimazu et al 2014). In that experiment, the total number of peripheral blood white blood cells (WBC), phagocyte activity (PA), complement alternative pathway activity (CAPA), granular leukocyte and lymphocytes ratio (GLR), and sheep red blood cell (SRBC)-specific antibody production (AP) were measured at approximately 105 kg body weight (BW), and breeding values were estimated.…”

Section: Introductionmentioning

confidence: 99%

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Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Sato

Okamura

Kojima-Shibata³

et al. 2015

Animal Science Journal

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Mycoplasma pneumonia of swine (MPS) is responsible for significant economic losses in the swine industry. We selected Landrace pigs for reduced MPS pulmonary lesions over five generations, and measured concentrations of the following cytokines: interleukin (IL)-10, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ to estimate their correlation with MPS lesions. Sheep red blood cells (SRBC) were injected twice intramuscularly at 70 and 95 kg body weight. Blood serum samples were collected after 1 week of secondary SRBC inoculation and cytokine concentrations were analyzed by ELISA. Genetic parameters and breeding values were estimated. The heritability estimates of IL-10, IL-13, IL-17, TNF-α and IFN-γ were 0.20 ± 0.06, 0.12 ± 0.06, 0.27 ± 0.07, 0.20 ± 0.10 and 0.05 ± 0.03, respectively. Genetic correlations of IL-17 and TNF-α with pulmonary MPS lesions were high (-0.86 ± 0.13 and 0.69 ± 0.29, respectively) and those of IFN-γ and IL-13 with MPS lesions were moderately negative (-0.45). Through selection, the breeding values of IL-17 and IFN-γ increased substantially and those of TNF-α decreased. These results suggest that innate and cellular immunity are more important for the suppression of pulmonary lesions in MPS than humoral-mediated immunity, such as antibody response.

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Section: Genetic Trendssupporting

confidence: 85%

Section: Genetic Trendsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Sato

Okamura

Kojima-Shibata³

et al. 2015

Animal Science Journal

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“…However, the search for significant and applicable gene variants has developed into an ever-expanding and successful branch of clinical research, including viral (Pseudorabiesvirus [88]; Influenza A (e.g. [143]); bacterial (Haemophilus parasuis [131]; Actinobacillus pleuropneumoniae [93,94]; Mycoplasma hyopneumoniae (e.g., [108]); Streptococcus suis [136]) and parasitic diseases (Sarcocystis [89]; Ascaris suum [100,101]). More than 2,500 quantitative trait loci (QTL) have been published for health parameters in the pig, among them 400 for resistance/susceptibility against a broad range of pathogens (http://www.animalgenome.org/QTLdb/; current status: October 2016).…”

Section: Examples On the Waymentioning

confidence: 99%

Genetic resistance - an alternative for controlling PRRS?

Reiner

2016

Porc Health Manag

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PRRS is one of the most challenging diseases for world-wide pig production. Attempts for a sustainable control of this scourge by vaccination have not yet fully satisfied. With an increasing knowledge and methodology in disease resistance, a new world-wide endeavour has been started to support the combat of animal diseases, based on the existence of valuable gene variants with regard to any host-pathogen interaction. Several groups have produced a wealth of evidence for natural variability in resistance/susceptibility to PRRS in our commercial breeding lines. However, up to now, exploiting existing variation has failed because of the difficulty to detect the carriers of favourable and unfavourable alleles, especially with regard to such complex polygenic traits like resistance to PRRS. New hope comes from new genomic tools like next generation sequencing which have become extremely fast and low priced. Thus, research is booming world-wide and the jigsaw puzzle is filling up – slowly but steadily. On the other hand, knowledge from virological and biomedical basic research has opened the way for an “intervening way”, i.e. the modification of identified key genes that occupy key positions in PRRS pathogenesis, like CD163. CD163 was identified as the striking receptor in PRRSV entry and its knockout from the genome by gene editing has led to the production of pigs that were completely resistant to PRRSV – a milestone in modern pig breeding. However, at this early step, concerns remain about the acceptance of societies for gene edited products and regulation still awaits upgrading to the new technology. Further questions arise with regard to upcoming patents from an ethical and legal point of view. Eventually, the importance of CD163 for homeostasis, defence and immunity demands for more insight before its complete or partial silencing can be answered. Whatever path will be followed, even a partial abolishment of PRRSV replication will lead to a significant improvement of the disastrous herd situation, with a significant impact on welfare, performance, antimicrobial consumption and consumer protection. Genetics will be part of a future solution.

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“…hyopneumoniae vaccine sensitization (Shimazu et al . , ). The Research and Development Center, Nippon Meat Packers Inc., Japan, improved the Large White breed by selecting for higher peripheral blood immunity over six generations.…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics of pig lines generated by crossing an MPS pulmonary lesion selected Landrace line and a highly immune capacity selected Large White line

Borjigin

Shimazu

Katayama

et al. 2015

Animal Science Journal

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To understand the influence of crossbreeding on Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics, two crossbred lines were characterized. One crossbred line, LaWa, was generated by crossing the MPS pulmonary lesion selected Landrace line (La) and the highly immune-selected Large White line (Wa). The second crossbred line, LaWb, was generated by crossing the La line and the nonselected Large White line (Wb). The crossbred LbWb line (nonselected Landrace line × nonselected Large White line) and the La line were used as controls. The LaWa and LaWb lines had an intermediate level of MPS lung lesions between La and LbWb lines, although the difference was not statistically significant. After stimulation with sheep red blood cells (SRBCs), the LaWb and LaWa lines showed immune characteristics similar to that of the La line; the number of monocytes in peripheral blood increased, while B cells, T cells, secretion of SRBC-specific immunoglobulin G, and interleukin (IL)-13 decreased. Additionally, the number of natural killer (NK) cells and the expression of IL-4 and IL-17 were significantly higher in the LaWb and LaWa lines, respectively. These data suggested that crossbreeding of La and Wa lines resulted in the inheritance of some of the selected immune responses.

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Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

Cited by 13 publications

References 36 publications

Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Genetic resistance - an alternative for controlling PRRS?

Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics of pig lines generated by crossing an MPS pulmonary lesion selected Landrace line and a highly immune capacity selected Large White line

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