Genetic Stability and Agronomic Evaluation of Six-Year-Old Transgenic Kiwi Plants for Rolabc and Rolb Genes

Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants

“…For transformation systems based on the development of adventitious shoots directly from leaf tissue, as we have used in our study, the production of chimeric plants was described several times (Rugini et al 1997;Matthews et al 1998). It was shown by Schmülling and Schell (1993) that transgenic plants regenerated from leaf disks and grown on selective media have not necessarily the same clonal origin.…”

Olive

“…These observations led to the hypothesis that putrescine acts during the root induction phase by means of an increase in peroxidase activity through H 2 O 2 . In fact, a drastic reduction of polyamines was observed at the basal part of able-to-root microcuttings, within 48 h after shoot preparation, even when they were cultured in putrescine-containing medium (Rugini et al, 1997).…”

“…Furthermore, in vitro propagation permits a high percentage of rooting of the explants even in those cultivars considered recalcitrant to propagation by cutting (Briccoli Bati and Lombardo, 1995). Exogenous treatment with putrescine is normally essential for rooting induction and for improving root system quality and plant growth (Rugini and Wang, 1986;Rugini, 1992;Rugini et al, 1997). The marked effect of putrescine on rooting is presumably due to a general low content of endogenous polyamines in olive shoots at the end of proliferation, in comparison with other woody species (e.g., walnut and chestnut), which are not affected by putrescine treatment (Rugini, 1992;Rugini et al, 1993).…”

“…The introduction of the parthenocarpy gene of Arabidopsis may allow fruit development and subsequently boost the yield, and may help to overcome the pollination problem as already successfully tried in eggplant (Rotino et al, 1997). In addition, the reduction of flower numbers observed in kiwi may contribute to solve the waste of energy and to reduce the alternate bearing (Rugini et al, 1997).…”

Γενετική μελέτη της έκφρασης του φύλου και της αυτοσυμβατότητας στη σποροφυτική επιλογή ακτινιδίου (cv. Tsechelidis) με χρήση κυτταρογενετικών και μοριακών μεθόδων

“…(Rugini et al, 1997;Fung et al, 1998;Wang et al, 2006). Μοριακές αναλύσεις των γενετικά τροποποιημένων φυτών του είδους A. eriantha, έδειξαν ότι τα μεταφερόμενα γονίδια είχαν ενσωματωθεί στο γένωμα και εκφράζονταν σε ιστούς φύλλων, βλαστών, ριζών, ανθέων και καρπών (Wang et al, 2006).…”