Genetic studies on reference strains of mutans streptococci

Ota, Fusao; Yamato, Masayuki; Hayashi, Masako; Ota, Masayuki; Koga, Tetsuro; Sherin, Ahmed; Mukai, Chiharu; Sakai, Kentaro; Yamamoto, Shigeru

doi:10.1078/0944-5013-00162

Cited by 4 publications

(7 citation statements)

References 20 publications

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“…pyogenes mentioned above, preparation of more mAbs is awaited to understand the immunobiology of oral streptococci responsible for dental caries and other streptococci responsible for tonsillitis, nephritis and other conditions in humans and animals. The number of serotypic variations by the mAbs are shown in table 4 and agrees well with that based on ribotype variations reported before [Ota et al, 2002].…”

Section: Serological Diversity Of Eight Serotypes Of Mutans Streptococcisupporting

confidence: 78%

“…After drying in the air, the membrane was exposed to UV light to fi x the DNA. Hybridisation was carried out as described previously [Ota et al, 2002] except that digoxigenin-labelled whole genomic DNA of reference strains was used as a probe.…”

Section: Eia For Antibody Specifi City and Serological Identifi Catiomentioning

confidence: 99%

“…rRNA probes have also demonstrated the existence of genotypic heterogeneity among mutans streptococcal isolates [Saarela et al, 1993]. Furthermore, Ota et al [2002] demonstrated genetic variations among 25 reference strains by ribotyping. There are no reports describing a complete set of mAbs which can identify 8 serotypes of the mutans group of streptococci and indicating serological variations similar to the genetic variations described earlier.…”

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confidence: 99%

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Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Ota

Ota²,

Mahmud³

et al. 2005

Caries Res

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A set of monoclonal antibodies were prepared by the conventional cell fusion of myeloma cells (SP2/0-Ag14) with spleen cells from BALB/c mice immunised with whole cells of a strain of mutans streptococci. Their specificities were examined against 35 reference strains of mutans streptococci, 34 reference strains of other oral streptococci and 8 reference strains of other microorganisms often inhabiting the oral cavity. Specificity was examined by enzyme immunoassay using whole cells. A total of 52 strains, consisting of 19 strains isolated in Japan, 19 strains isolated in Italy and 14 strains isolated in England, were characterised by conventional physiological and biochemical tests and then serotyped by the use of 8 monoclonal antibodies with different specificities. They were also confirmed by guanine-plus-cytosine contents of their nucleic acid and DNA-DNA hybridisation test. The results indicated that all monoclonal antibodies are useful for identification of 8 serotypes of the mutans streptococci responsible for dental caries. They also suggest the existence of more serological varieties among mutans species.

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Section: Serological Diversity Of Eight Serotypes Of Mutans Streptococcisupporting

confidence: 78%

Section: Eia For Antibody Specifi City and Serological Identifi Catiomentioning

confidence: 99%

mentioning

confidence: 99%

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Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Ota

Ota²,

Mahmud³

et al. 2005

Caries Res

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“…Importantly, these transmission patterns were detected despite the limited number of available isolates per subject, and may thus reflect an underestimation of the true level of homology among ms strains from family members. A recent study (25) suggested that S. mutans is genetically more diverse than S. sobrinus, a finding that may partly explain the higher homology of intrafamilial strains of S. sobrinus as well as the occurrence of an identical S. sobrinus ribotype in two unrelated subjects. This observation is in agreement with other studies (8)(9)(10)(11)(12).…”

Section: Discussionmentioning

confidence: 99%

Longitudinal study of intrafamilial mutans streptococci ribotypes

Köhler

Lundberg

Birkhed

et al. 2003

European J Oral Sciences

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To explore the intrafamilial homology and longitudinal stability of colonization by early acquired mutans streptococci, genomic fingerprinting was performed on 254 strains (192 Streptococcus mutans and 62 Streptococcus sobrinus strains) collected from 16 families (16 mother-child pairs, seven fathers and four siblings). Genomic DNA was digested by the restriction endonuclease HindIII, followed by gel electrophoresis, Southern blotting, and hybridization with a digoxigenin-labeled 16S rDNA probe, and hybrid detection by enhanced chemiluminescence. One to five ribotypes were identified per person, and between two and nine (median five) within each family. Fourteen of the 16 mother-child pairs showed homology for at least one ribotype (range 1-4). Six of the seven father-child pairs had one ribotype in common. Ten of the 13 longitudinally examined children showed persistence of at least one ribotype over a period of up to 16 yr. The results support the notion of intrafamilial transfer of mutans streptococci, and suggest that colonization of early acquired strains persists into young adulthood.

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“…Newer systems are being developed such as terminal restriction fragment-length polymorphism (T-RFLP) [11,12], digoxigenin-labelled peptide nucleic acid to detect lactobacilli PCR amplicons immobilized on membranes from denaturing gradient gel electrophoresis [13] and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindromic PCR (REP-PCR) [14]. This is important in order to differentiate reliable and proven strains and products from those of dubious quality as referenced above.…”

Section: Step One: Identify the Exact Strain That Is Being Usedmentioning

confidence: 99%

The Importance of Guidelines in the Development and Application of Probiotics

Reid

2005

CPD

160

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Probiotics, defined as "Live microorganisms which when administered in adequate amounts confer a health benefit on the host" have many attributes including the lack of adverse side effects associated with their use. While probiotics have proven benefits, the optimism associated with their use is counterbalanced by the fact that many so-called "probiotic" products are unreliable in content and unproven clinically. Therefore much remains to be done to gain the acceptance of the broader medical community. Recognition of the obvious product inequality and the lack of any regulatory guidelines lead to the development of Operating Standards in 2002 (FAO/WHO), that would ensure product safety, reliability and a level playing field for all companies producing probiotic products. The guidelines constitute a set of parameters required for a product/strain to be termed "probiotic" and also the clinically relevant steps to be followed to move probiotics closer to being embraced by the medical community. These include i) implementation of Guidelines for use of probiotics; ii) phase I, II and III clinical trials to prove health benefits that are as good as or better than standard prevention or treatments for a particular condition or disease; iii) Good Manufacturing Practice and production of high quality products; iv) studies to identify mechanism of action in vivo; v) informative/ precise labelling; vi) development of probiotic organisms that can carry vaccines to hosts and/or anti- viral probiotics; vii) expansion of proven strains to benefit the oral cavity, nasopharynx, respiratory tract, stomach, vagina, bladder and skin as well as for cancer, allergies and recovery from surgery/ injury.

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Genetic studies on reference strains of mutans streptococci

Cited by 4 publications

References 20 publications

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Longitudinal study of intrafamilial mutans streptococci ribotypes

The Importance of Guidelines in the Development and Application of Probiotics

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