Genetic use restriction technologies and possible applications in the integrated pest management

Dalazen, Giliardi; Júnior, Aldo Merotto

doi:10.1590/0103-8478cr20160105

Cited by 3 publications

(2 citation statements)

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“…The lowest values of the Bt protein recorded in the Kaveri seeds indicate the possible role of the terminator technology. 25 The content of Cry1Ac in the corn samples was found to be 5.7 ± 0.30 μg in comparison with 1.4 μg g −1 in transgenic Bt 11 corn, reported by the International Cotton Advisory Committee, based on the bioassay toxicity studies. 24 Further, the Cry1Ac protein content determined in the leaves in the present study (1.76 ± 0.15 μg g −1 in CS10 and 1.6 ± 0.15 μg g −1 in CS4 variety was lower than those which exhibited Cry1Ac content of 0.20-0.29 g g −1 and a mean value of 0.24 g g −1 fresh tissue during the seasonal variations reported by the International Cotton Advisory Committee.…”

Section: Discussionmentioning

confidence: 79%

“…However, the F2 generation (Kaveri seeds) procured from Kondur, Warangal, showed comparatively lower Bt content (2.9 ± 0.06 μg). The lowest values of the Bt protein recorded in the Kaveri seeds indicate the possible role of the terminator technology . The content of Cry1Ac in the corn samples was found to be 5.7 ± 0.30 μg in comparison with 1.4 μg g −1 in transgenic Bt 11 corn, reported by the International Cotton Advisory Committee, based on the bioassay toxicity studies .…”

Section: Discussionmentioning

confidence: 99%

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Immuno‐analytical method development for detection of transgenic Cry1Ac protein and its validation

et al. 2019

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BACKGROUND Bacillus thuringiensis (Bt) synthesizes Cry1Ac protein, which is toxic to many lepidopteran pests, and the cry1ac gene has been expressed in several transgenic crop plants. The Cry1Ac protein has been isolated from Bt kurstaki HD73 and purified to homogeneity. Polyclonal antibodies were raised against purified Cry1Ac in rabbits and goat. Sandwich ELISA was developed for Cry1Ac using goat IgG as a coating antibody, and affinity‐purified rabbit IgG as the primary antibody. RESULTS The sensitivity of the assay was in the range of 0.47–1000 ng. It was subsequently employed in validating biological samples. Fifteen different cotton‐seed samples were screened: 12 were found to be Bt positive and 3 Bt negative. The CS7 seeds showed the highest Bt content of 8.51 ± 0.45 μg g−1, followed by CS8 (6.0 ± 0.02 μg g−1), CS15 (5.9 ± 0.03 μg g−1), CS9 (5.5 ± 0.05 μg g−1), and CS10 (4.83 ± 0.013 μg g−1). The CS5 seeds showed Bt content of 3.6 ± 0.21 μg g−1. The F2 generation, CS6 (Kaveri seeds) showed lower Bt content (2.9 ± 0.06 μg g−1). The CL5 samples showed Cry1Ac content of 0.99 ± 0.009 μg g−1. The amount of Cry1Ac protein in leaves, stem, and roots of germinated Bt cotton plants (CS10 and CS4) were 1.76 ± 0.15 μg g−1, 1.9 ± 0.01 μg g−1, 2.0 ± 0.1 μg g−1, and 1.6 ± 0.15 μg g−1, 1.9 ± 0.01 μg g−1, and 2.0 ± 0.01 μg g−1 dry tissue, respectively. CONCLUSION The method developed can be used for screening the expression levels of Cry1Ac in different transgenic Bt cultivars and also spurious Bt cotton seeds procured by farmers. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Immuno‐analytical method development for detection of transgenic Cry1Ac protein and its validation

et al. 2019

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The current status of the use of genetic modification and editing to improve biodiversity and ecological sustainability

Mmbando,

Ngongolo

2024

All Life

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Technologies of Genetic Material Use Restriction: Types, Molecular-Genetic Base and Ethical Analysis of Their Application

Zubik¹

2021

Biotechnol. acta

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In order to save money, some farms use the seed obtained in the process of cultivation not only for sale, but also for sowing, which has not found supporters among companies engaged in the production of genetically modified seed. To protect their rights, the latter have created technologies to limit the use of genetic material, which are intended to be used for protection the intellectual rights to reproduce plants with a changed genotype. However, these technologies contain also a commercial component and violate a number of moral principles and international acts. Aim. To describe the types of terminator technologies, their genetic and molecular basis and purpose. To assess a correspondence of their compliance with the international documents and norms. Method. Terminator technologies types, genetic bases and application and their analysis from the standpoint of international norms were studied. To achieve the goal, the methods of fact analysis, comparison and generalization were used. Results. There are two types of terminator technologies (variety- and trait-specific), which are based on the interaction of three genes, which leads to the implementation of certain phenotypic manifestations. It was found that the technologies for limiting the use of genetic material are both contradictory and consistent with a number of international legal acts, which did not make it possible to determine clearly the appropriateness of their use in agriculture. Conclusions. Terminator technologies application is still a controversial fact since they are based on the duality principle: to carry simultaneously a positive and a negative manifestation for people.

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Genetic use restriction technologies and possible applications in the integrated pest management

Cited by 3 publications

References 33 publications

Immuno‐analytical method development for detection of transgenic Cry1Ac protein and its validation

Immuno‐analytical method development for detection of transgenic Cry1Ac protein and its validation

The current status of the use of genetic modification and editing to improve biodiversity and ecological sustainability

Technologies of Genetic Material Use Restriction: Types, Molecular-Genetic Base and Ethical Analysis of Their Application

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