Genetic variability and phylogenetic aspects in species of the genus Macrobrachium

Abstract

ABSTRACT. The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and phylogenetic relationship between Macrobrachium amazonicum and Macrobrachium jelskii, which are closely related species. Additionally, they are syntopics and their taxonomy poses problems because it is difficult to differentiate between the species. We used the mitochondrial gene sequences COI and 16S rRNA to assess the genetic structure of these species in 3 populations that were collected from Tiete hydrographic basin (São Paulo State, Brazil). The interspecific Genetic variability in the genus Macrobrachium comparison of samples that were collected at the same and different locations showed a low rate of genetic variability. This similarity was attributed to the recent introduction of these species in the regions that were sampled and the habitat conditions in which they inhabit. In addition, these results may be consistent with the hypothesis that they are a single species, interspecific hybrids, or metapopulation. The dendrogram analyses did not reveal the formation of clusters, confirming the disturbances in the genetic structure of the samples that were analyzed in this study. These data are pioneers to these crustaceans, and they confirm the ecological and evolutionary problems between these Macrobrachium species.

Genetic variation of giant freshwater prawns Macrobrachium rosenbergii wild population of South Sulawesi, Indonesia

“…The results of the 700 bp giant freshwater prawn DNA amplification (Figure 2) are consistent with those obtained by Jimoh et al (2013) using the same primer. In addition, other species had ranges that were similar to the results of the DNA amplification, such as 680 bp in Macrobrachium amazonicum and Macrobrachium jelskii (Guerra et al 2014), 710 bp in Macrobrachium lanchesteri (Khanarnpai et al 2019), and 650 bp in Macrobrachium and Caridina (Udayasuriyan et al 2017).…”

“…Several methods for analyzing DNA are available, including Random Amplified Polymorphism DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Microsatellite DNA, DNA fingerprinting, and mt-DNA. Alam et al (2017), Chen et al (2015Chen et al ( , 2017, Guerra et al (2014), and Khanarnpai et al (2019) have used mt-DNA on Macrobrachium. The COI mt-DNA fragments and 16SrRNA D-loop mt-DNA sequences are widely used in population genetic analysis because they do not encode proteins and are unaffected by the selection (Liao et al 2016;Maltsev et al 2015).…”

New Record of Macrobrachium lar (Fabricius, 1798) (Crustacea: Decapoda: Palaemonidae) from Al-Hammar Marsh, Southern Iraq

“…Macrobrachium Bate, 1868 is a widely distributed and abundant macroinvertebrate genus in inland water. It has been recorded in several tropical and subtropical regions such as lakes, ponds, rivers, as well as in estuaries (Cook et al, 2002;Guerra et al, 2014). Species of Macrobrachium found in lowlands of Africa, Asia, Oceania, North, Central and South America.…”

“…Although most of the species are inhabiting freshwater environments, some can tolerate higher salinities and found near sea coasts where water is rather brackish (Wowor et al, 2009;Pileggi & Mantelatto, 2010). Update, this genus includes approximately 210 species of prawns and is considered as the largest palaemonid genus, with the greatest diversity occurring in the Indo-Pacific region and the Americas (Mantelatto et al, 2008;De Grave & Fransen, 2011;Guerra et al, 2014).…”