Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited

Abstract

Background: Bamboo is among the important plants that help shape the socio-economic fabric of rural India. It provides employment, sustains business ventures, has medicinal applications and even helps in carbon sequestration. Out of 125 indigenous species, Dendrocalamus strictus (Roxb.) Nees occupies 53% of the area of bamboo in the country. Moreover, D. strictus may be used in afforestation of wastelands and rural development programmes due to its adaptability in wider landscapes. Dendrocalamus strictus has different growth forms based on edaphic factors and climatic conditions. DNA profiling was used to analyse the genetic diversity among the different growth forms of D. strictus present in three different locations of Uttarakhand. Methods: The study area includes three locations, first, reserve forest of the Forest Research Institute (FRI), Dehradun; second, Shivpuri near Byasi, Rishikesh; and third, Chiriapur range (Haridwar district). A standard method was used to isolate DNA from young leaves from ten clumps of each growth form. Ten RAPD primers were screened for polymorphism from A and N operon primers and a standard PCR protocol was followed to amplify and visualise DNA bands. The data matrix was analysed and interpreted using statistical software and methods. Results: The cluster analysis, genetic structure parameters, moderate coefficient of gene differentiation and low gene flow value all indicated that these growth forms are genetically dissimilar and that geographic separation as well as physiological/flowering barriers has influenced these variations. These genetically different growth forms can be called ecotypes. Conclusions: Such a study has not been attempted previously with bamboo and will help inform the conservation of the genetic pool of bamboo ecotypes. Seeds of these ecotypes are monocarpic in nature, which means that bamboo plants flower once in their lifetime, so they must be collected and multiplied (as plantations) in their respective habitats.

Aromatic Profile Variation of Essential Oil from Dried Makwhaen Fruit and Related Species

“…For RAPD analysis of the genomic DNA, 10-base primers from Operon Technologies (Alameda, GA, USA) and UBC (University of British Columbia, Canada) were chosen ( Table S4 ). A total of nine primers from previous studies were screened [ 43 , 44 , 45 , 46 ]. The polymerase chain reaction (PCR) was adjusted to 10 μL −1 containing 8 μL −1 of OnePCRTM Plus (Genedirex, Taoyuan, Taiwan), 1 μL −1 of 1 μm RAPD primer and 1 μL −1 of 10 ng genomic DNA.…”

Effect of growth forms of bamboo on the mycorrhizal and fluorescent pseudomonas population

“…The study was conducted at three locations: first, reserve forest of the FRI, Dehradun (latitudes of 30° 20’N and longitudes of 78° 00’E); second, Shivpuri near Byasi Rishikesh (latitudes of 30° 06’N and longitude of 78° 25’E); and third, Chiriapur range, Haridwar district (between the latitude of 29° 52’N and longitude of 78° 11’E). These locations were selected based on the presence of different growth forms of D. strictus (Das et al, 2017). Climatic and soil physico-chemical parameters were also recorded (Table 1).…”

“…These variations, evolved due to the environmental and other factors, are a matter of investigation. A study conducted by Das et al (2017) explored the genetic variability among and within growth forms of bamboo using RAPD.…”

Population structure and functional diversity of mycorrhizal fungi and Pseudomonas fluorescence in different growth forms of Dendrocalamus strictus

“…These variations, evolved due to the environmental and other factors, are a matter of investigation. A study on the genetic bamboo using RAPD was done by Das et al, (2017).One of the important problems in tropical biomass production is the low-soil-phosphate (P) availability.…”