Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

Ishikawa, Francine Hiromi; Souza, Elaine Aparecida de; Davide, Livia Maria Chamma

doi:10.2478/s11756-008-0039-6

Cited by 21 publications

(18 citation statements)

References 16 publications

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“…Incompatibility could be a mechanism of self-defense (Glass et al, 2000). The large number of anastomosis groups was in agreement with the results obtained by RodriguezGuerra et al (2003) and Ishikawa et al (2008), who showed variability for the isolates of C. lindemuthianum studied and a great variation in the number of anastomosis groups.…”

Section: Discussionsupporting

confidence: 91%

“…Some studies have been done for the common bean fungus Colletotrichum lindemuthianum, from which several anastomosis groups have been identified in natural populations (Rodríguez-Guerra et al, 2003;Ishikawa et al, 2008). According to the authors, the occurrence of anastomosis could be an important mechanism that can lead to the formation of different lineages of C. lindemuthianum.…”

Section: Angular Leaf Spot Of Common Bean (Phaseolus Vulgaris L) Is mentioning

confidence: 99%

“…According to the authors, the occurrence of anastomosis could be an important mechanism that can lead to the formation of different lineages of C. lindemuthianum. Genetically different isolates of C. lindemuthianum showed the capacity for hyphal anastomosis between one another, which could contribute to increased genetic variability (Ishikawa et al, 2008). However, studies using nit mutants showed a predominance of vegetative incompatibility in C. lindemuthianum (Castro-Prado et al, 2007;Barcelos et al, 2011), and despite anastomosis formation, vegetative incompatibility reaction occurs afterwards, leading to cell death (Ishikawa et al, 2012).…”

Section: Angular Leaf Spot Of Common Bean (Phaseolus Vulgaris L) Is mentioning

confidence: 99%

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Anastomosis groups and molecular variation in Pseudocercospora griseola

et al. 2015

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ABSTRACT. The fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P. grisola isolates and to evaluate the variability of isolates using analyses of anastomosis groups and RAPD markers. Hyphal anastomosis was observed in all isolates. Three isolates showed 85.0% compatibility and were compatible with 17 isolates. This is the first report of the occurrence of anastomosis between P. griseola isolates. 7437 Variation in Pseudocercospora griseola©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7436-7445 (2015) Sixteen anastomosis groups were observed and only one group was formed by five isolates (Pg-01, Pg-02, Pg-07, Pg-08, and Pg-12). There was a large number of anastomosis groups and absence of clustering among isolates for hyphal fusion, demonstrating the existence of genetic variability for loci involved in the control of this trait. High genotypic variability by RAPD markers was detected, but there was no relationship between genotype and anastomosis group. More studies are necessary to elucidate further the process of asexual recombination in this phytopathogen.

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Section: Discussionsupporting

confidence: 91%

Section: Angular Leaf Spot Of Common Bean (Phaseolus Vulgaris L) Is mentioning

confidence: 99%

Section: Angular Leaf Spot Of Common Bean (Phaseolus Vulgaris L) Is mentioning

confidence: 99%

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Anastomosis groups and molecular variation in Pseudocercospora griseola

et al. 2015

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“…Likewise, Ishikawa, Souza, and Davide (2008) worked with 13 isolates of race 65 from the State of Minas Gerais from different regions and years and used RAPD analysis to identify 11 groups with the phylogenetic tree, which demonstrates the presence of variability within race 65. Santos, Antunes, Rey, and Rosseto (2008) reported pathogenic variability within isolates of race 65, 73 and 81, and two isolates of the same race were inoculated into 37 cultivars used in this study.…”

Section: Similarity and Dissimilarity Measuresmentioning

confidence: 99%

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

et al. 2016

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ABSTRACT. The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and 3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic tree obtained based on the sequences of the ITS1 and ITS2 regions revealed the formation of two groups, one with a subgroup. The results reveal high molecular variability among isolates of race 65 of C. lindemuthianum.Keywords: anthracnose, genetic variability, pathogens, dissimilarity.Caracterização da raça 65 de Colletotrichum lindemuthianum utilizando sequenciamento das regiões ITS RESUMO. O presente trabalho teve como objetivo caracterizar isolados de C. lindemuthianum da raça 65 provenientes de diversas regiões do Brasil, por meio de sequenciamento de regiões ITS. Um total de 17 isolados da raça 65, coletados nos estados do Mato Grosso, Minas Gerais, Paraná, Santa Catarina e São Paulo, foram estudados. As análises das sequências dos isolados 8, 9, 12, 14 e 15 revelaram a presença de dois SNPs na região ITS1 nas mesmas posições. Estes mesmos isolados quando analisados juntamente com a sequência do isolado 17 apresentaram um SNP na região ITS2. A maior dissimilaridade genética foi de 0,772 observada entre os isolados 11 e 3 e entre os isolados 11 e 10. Por sua vez, os isolados 7 e 2 foram os mais similares, com valor de distância genética de 0,002. A árvore filogenética obtida com base nas sequências das regiões ITS1 e ITS2 revelou a formação de dois grupos, sendo um com a divisão de um subgrupo. Estes resultados revelam uma elevada variabilidade molecular entre isolados da raça 65 de C. lindemuthianum.

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“…The most economically viable and effective strategy for disease control is by using bean cultivars resistant to fungi (Ishikawa et al, 2008). However, this kind of management is difficult to implement due to the high pathogenic variability of C. lindemuthianum (Ishikawa et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Essential oil of Cymbopogon flexuosus, Vernonia polyanthes and potassium phosphite in control of bean anthracnose

Silva¹,

Souza²,

Alves³

et al. 2015

J. Med. Plants Res.

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This study evaluated essential oils of Cymbopogon flexuosus (EOC), Vernonia polyanthes (EOV), and potassium phosphite (PP) in control of bean anthracnose caused by Colletotrichum lindemuthianum. This study assessed mycelial growth and conidial germination in vitro, scanning electron microscopy (SEM), and enzymes peroxidase (POX) and phenylalanine ammonia-lyase (PAL). EOC and EOV in doses 1,450 and 1,320 μL L -1 reduced disease severity by 57.2 and 37.6%, respectively. Major components identified in EOC were geraniol 46.8% and nerol 33.4%, and germacrene-D (42.2%) and bicyclogermacrene (17.2%) in EOV. SEM images showed that PP, EOC and EOV reduced mycelial growth and emission of germ tubes. PP and EOC increased POX and PAL rates in bean tissue. Bean anthracnose was controlled by direct antifungal activity of PP and EOC and induction of defense enzymes

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Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

Cited by 21 publications

References 16 publications

Anastomosis groups and molecular variation in Pseudocercospora griseola

Anastomosis groups and molecular variation in Pseudocercospora griseola

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

Essential oil of Cymbopogon flexuosus, Vernonia polyanthes and potassium phosphite in control of bean anthracnose

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