Genetic variation among a world-wide collection of isolates of Fusarium oxysporum f. sp. cubense analysed by RAPD-PCR fingerprinting

Bentley, Sharon A; Pegg, K. G.; Dale, James L.

doi:10.1016/s0953-7562(09)81225-2

Cited by 85 publications

(66 citation statements)

References 17 publications

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“…One would, therefore, expect that the rest of the genomes of individuals in one vegetative compatibility group (VCG) of an asexual species would also be very similar (Leslie, 1990;Leslie, 1993). Although individuals in VCGs of F. oxysporum are related by clonal descent (Gordon and Martyn, 1997;Leslie, 1993), a mutation at a single vic locus would place closely related individuals in different VCGs (Bentley and Dale, 1995;Bentley et al, 1998). Thus, VCGs represent good phenotypic characters for assessing diversity within populations, but genetic relationships among VCGs must be assessed by other means.…”

Section: Characterization Of Formae Speciales Based On Vegetative Commentioning

confidence: 99%

Current status of the taxonomic position of Fusarium oxysporum formae specialis cubense within the Fusarium oxysporum complex

Fourie

Steenkamp

Ploetz

et al. 2011

Infection, Genetics and Evolution

129

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Fusarium oxysporum is an asexual fungal species that includes human and animal pathogens and a diverse range of nonpathogens. Pathogenic and nonpathogenic strains of this species can be distinguished from each other with pathogenicity tests, but not with morphological analysis or sexual compatibility studies. Substantial genetic diversity among isolates has led to the realization that F. oxysporum represents a complex of cryptic species. Fusarium oxysporum f. sp cubense (Foc), causal agent of Fusarium wilt of banana, is one of the more than 150 plant pathogenic forms of F. oxysporum. Multi-gene phylogenetic studies of Foc revealed at least eight phylogenetic lineages, a finding that was supported by random amplified polymorphic DNAs, restriction fragment length polymorphisms and amplified fragment length polymorphisms. Most of these lineages consist of isolates in closely related 2 vegetative compatibility groups, some of which possess opposite mating type alleles, MAT-1 and MAT-2; thus, the evolutionary history of this fungus may have included recent sexual reproduction. The ability to cause disease on all or some of the current race differential cultivars has evolved convergently in the taxon, as members of some races appear in different phylogenetic lineages. Therefore, various factors including co-evolution the plant host and horizontal gene transfer are thought to have shaped the evolutionary history of Foc. This review discusses the evolution of Foc as a model formae specialis in F. oxysporum in relation to recent research findings involving DNA-based studies.

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Section: Characterization Of Formae Speciales Based On Vegetative Commentioning

confidence: 99%

Current status of the taxonomic position of Fusarium oxysporum formae specialis cubense within the Fusarium oxysporum complex

Fourie

Steenkamp

Ploetz

et al. 2011

Infection, Genetics and Evolution

129

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“…Based on vegetative compatibility, F. oxysporum f. sp. cubense isolates have been separated into 24 so-called vegetative compatibility groups (VCGs) (5,29,47,68). Finally, various DNA-based tools have been used to separate F. oxysporum f. sp.…”

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confidence: 99%

Evolutionary Relationships among theFusarium oxysporumf. sp.cubenseVegetative Compatibility Groups

Fourie

Steenkamp

Gordon

et al. 2009

Appl Environ Microbiol

145

204

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Fusarium oxysporum f. sp. cubense, the causal agent of fusarium wilt of banana (Musa spp.), is one of the most destructive strains of the vascular wilt fungus F. oxysporum. Genetic relatedness among and within vegetative compatibility groups (VCGs) of F. oxysporum f. sp. cubense was studied by sequencing two nuclear and two mitochondrial DNA regions in a collection of 70 F. oxysporum isolates that include representatives of 20 VCGs of F. oxysporum f. sp. cubense, other formae speciales, and nonpathogens. To determine the ability of F. oxysporum f. sp. cubense to sexually recombine, crosses were made between isolates of opposite mating types. Phylogenetic analysis separated the F. oxysporum isolates into two clades and eight lineages. Phylogenetic relationships between F. oxysporum f. sp. cubense and other formae speciales of F. oxysporum and the relationships among VCGs and races of F. oxysporum f. sp. cubense clearly showed that F. oxysporum f. sp. cubense's ability to cause disease on banana has emerged multiple times, independently, and that the ability to cause disease to a specific banana cultivar is also a polyphyletic trait. These analyses further suggest that both coevolution with the host and horizontal gene transfer may have played important roles in the evolutionary history of the pathogen. All examined isolates harbored one of the two mating-type idiomorphs, but never both, which suggests a heterothallic mating system should sexual reproduction occur. Although, no sexual structures were observed, some lineages of F. oxysporum f. sp. cubense harbored MAT-1 and MAT-2 isolates, suggesting a potential that these lineages have a sexual origin that might be more recent than initially anticipated.

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“…The highly similar banding patterns generated in both analyses showed close relationship among the FOC isolates, as no correlation was found between the banding patterns and the banana cultivars and locations. A study by [2] found that DNA fingerprinting revealed that the FOC isolates were generally VCG specific and neither correlates with location nor banana cultivar. In another study [1] , also pointed out that genetic profile of F. oxysporum from tomato plants did not correlate with locations.…”

Section: Discussionmentioning

confidence: 99%

“…Bentley et al [2] suggested that genetic variation in FOC might be due to adaptation and co-evolution of the fungus with the host and environmental factors of the location.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Fusarium Oxysporum F. Sp. Cubense of Banana

Leong¹,

Zakaria²,

Baharuddin³

2009

American J. of Applied Sciences

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Problem statement: Morphological characterization of Fusarium species which emphasize on microscopic and cultural characteristics are not sufficient to characterize Fusarium Oxysporum F. sp. Cubense (FOC) from banana as these characteristics could easily influence by environmental factors. As an alternative molecular methods were used to characterize and to assess genetic variation of FOC from different banana cultivars. Knowledge on the genetic variation is important to determine the genetic relationship between FOC isolates from different banana cultivars. Approach: Two PCRbased methods, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) and restriction analysis of the Internal Transcribed Spacer and 5.8S regions (ITS+5.8S regions) were used to characterize Fusarium Oxysporum F. sp. Cubense (FOC) isolates from different banana cultivars. The genetic relationship of the FOC isolates were analyzed using Un-weighted Pair-Group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient. Results: Restriction patterns of the ITS+5.8S regions using nine restriction enzymes namely, Alu I, Eco RI, Eco 88I, Bsu RI, Bsu 15I, Hin fI, Hin 6I, Msp I and Taq I and ERIC-PCR showed low variation among the FOC isolates studied, indicating close relationship among the isolates. Unweighted Pair-group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient showed that the FOC isolates were grouped into two main clusters with similarity value of 41.4-100% in PCR-RFLP of ITS + 5.8S and 45-100% similarity based on ERIC-PCR analysis, respectively. Cluster analysis of the combined data also showed that the FOC isolates were grouped into two clusters, sharing 42.9-100% similarity. Conclusion/Recommendations: The results of the present study indicate that the FOC isolates were closely related regardless of banana cultivars and location.

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Genetic variation among a world-wide collection of isolates of Fusarium oxysporum f. sp. cubense analysed by RAPD-PCR fingerprinting

Cited by 85 publications

References 17 publications

Current status of the taxonomic position of Fusarium oxysporum formae specialis cubense within the Fusarium oxysporum complex

Current status of the taxonomic position of Fusarium oxysporum formae specialis cubense within the Fusarium oxysporum complex

Evolutionary Relationships among theFusarium oxysporumf. sp.cubenseVegetative Compatibility Groups

Molecular Characterization of Fusarium Oxysporum F. Sp. Cubense of Banana

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