Genetic variation based on microsatellite analysis of the oriental river prawn, Macrobrachium nipponense from Qiandao Lake in China

Y, K; Feng, Jianbin; Li, J L

doi:10.4238/2012.september.20.1

Cited by 16 publications

(23 citation statements)

References 31 publications

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“…Microsatellites are codominant, highly polymorphic, and ideal for genetic diversity, population structure and genetic mapping studies (Ma et al, 2012;Liu et al, 2013). A few microsatellites are available for C. alburnus (Chen et al, 2009;Li et al, 2010;Qi et al, 2013 ), but unfortunately, the number of SSRs available for this species is limited.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)

Liu¹,

Gu²,

Jia³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA) 12 and (GA) 12 probes. The variability of these microsatellites was tested on 30 individuals cultured. The average allele number was 6.6 per locus, ranging from 3 to 12. The observed heterozygosity was from 0.4667 to 0.9000, and the expected heterozygosity was from 0.6163 to 0.9085. After using Bonferroni's correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci, but deviations from HardyWeinberg equilibrium were found in 3 loci. These microsatellites can be used to study QTL of economic importance, population genetic diversity and the construction of genetic maps for C. alburnus in the future.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)

Liu¹,

Gu²,

Jia³

et al. 2014

Genet. Mol. Res.

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show abstract

“…In this study, F ST values were 0.029-0.143, which is high compared with other fishery species (Li et al, 2006;Zhan et al, 2008;Ma et al, 2012). Long periods of geographical and ecological isolation, in addition to random mating within the natural habitat, may explain the genetic differentiation of M. meretrix (Lin, 2007).…”

Section: Molecular Distinction and Relationships Among The 5 Populationsmentioning

confidence: 54%

“…The quality of offspring is mainly determined by additive genetic factors (Liu and Cordes, 2004). Highly abundant genetic variation contributes to the survival of populations as an adaptation to environmental change (Ma et al, 2012). In this study, the SD and JS populations showed the least variation.…”

Section: Molecular Distinction and Relationships Among The 5 Populationsmentioning

confidence: 59%

“…Such deviations may be caused by heterozygote deficiency (Zhan et al, 2008), which is commonly observed in microsatellite loci (Sato et al, 2005;Zhan et al, 2008;Lu et al, 2012a), and may be caused by sample size, non-random mating because of water flow (Ma et al, 2012), or null alleles (Callen et al 1993;Zhan et al, 2008). Population genetic analyses of HWE may be compromised because of the existence of false homozygotes.…”

Section: Genetic Polymorphism and Genetic Variationmentioning

confidence: 99%

“…Microsatellite markers have been widely used because of their hypervariability, ubiquitous presence, neutrality, abundance, and co-dominance (Tautz, 1989;Weber and May, 1989;An et al, 2012;Ma et al, 2012). Simple sequence repeat loci (microsatellites) identified from the expressed sequence tag database (EST-SSR markers) are generally related to a particular function, such as resistance or growth.…”

Section: Introductionmentioning

confidence: 99%

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Microsatellite marker analysis reveals the distinction between the north and south groups of hard clam (Meretrix meretrix) in China

Dong

Yao

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Meretrix meretrix is one of the important commercial bivalves in China. A total of 198 individual clams were collected from 5 locations characteristic of the clam's 5 main natural habitats in China, that is, Shandong, Jiangsu, Fujian, Guangdong, and Guangxi. Ten polymorphic microsatellite markers were selected to examine the genetic diversity and identify genetic differences between the 5 populations. A total of 183 alleles across 10 loci were detected in the individual clams. The observed heterozygosity and expected heterozygosity ranged from 0.197 to 0.7026 and from 0.6264 to 0.9408, respectively. The genetic diversity within samples was high (8.6-11.2 alleles per locus, observed heterozygosity = 0.25-0.875 and expected heterozygosity = 0.6848-0.9259). Most of the genotype distributions significantly deviated from Hardy-Weinberg equilibrium. Genetic structure analysis showed that the 5 populations could be divided into 2 groups, the north and south groups. Neighbor-joining analysis revealed a clear distinction between the north group (Shandong and Jiangsu) and the south group (Fujian, Guangdong, and Guangxi). Locus MM1031 was used to distinguish between groups. Our results can be used for population identification and crossbreeding of M. meretrix.

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Genetic diversity and population structure of wild Macrobrachium nipponense populations across China: Implication for population management

et al. 2023

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Macrobrachium nipponense, is an important economic indigenous prawn in China, which is widely distributed in freshwater in China, Japan and Southeast Asia. In this study, D-loop region sequences was used to investigate the genetic diversity and population structure of 22 wild populations of M. nipponense through China, containing the major rivers and lakes of China. Totally 475 valid D-loop sequences from 22 populations with a length of 1110bp were obtained, and 348 variation sites and 221 haplotypes were detected. A total of 221 haplotypes were defined in 22 populations.Hap48 was the dominant haplotype, accounting for about 13%. The haplotype diversity (h) was ranged from 0.1630 (BN) ~1.0000 (AR) and the nucleotide diversity π value ranged from 0.001164 (MR) ~ 0.037168 (NR). The pairwise genetic differentiation index (FST) ranged from 0.00344 to 0.91243 and most pair-wised FST was significant (P < 0.05). The lowest FST was displayed in MR and JLR population and the highest was between NDR and NR. The phylogenetic tree of genetic distance based on UPGMA showed that all populations were divided into two branches. The DC, NDR, JLR and MR populations were clustered into one branch. The neutral test and mismatch distribution results showed that M. nipponense populations were not experienced expanding and kept a steady increase. Taken together, a joint resources protection and management strategy for M. nipponense have been suggested based on the results of this study for its sustainable use.

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Genetic variation based on microsatellite analysis of the oriental river prawn, Macrobrachium nipponense from Qiandao Lake in China

Cited by 16 publications

References 31 publications

Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)

Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)

Microsatellite marker analysis reveals the distinction between the north and south groups of hard clam (Meretrix meretrix) in China

Genetic diversity and population structure of wild Macrobrachium nipponense populations across China: Implication for population management

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