Genetic variation for seed storage protein composition in rapeseed (Brassica napus) and development of near‐infrared reflectance spectroscopy calibration equations

Abstract

Rapeseed protein consists mainly of the seed storage protein cruciferin and napin. Cruciferin and napin have different nutritional values and techno‐functional properties. Shifting the cruciferin/napin ratio towards either more napin or more cruciferin could allow diversified applications. The objective of this study was to investigate the genetic variation of cruciferin and napin in modern winter rapeseed cultivars. Cruciferin and napin contents were analysed by SDS‐PAGE. Genetic variation for both protein fractions was highly significant. Heritabilities were high ranging from 74% for cruciferin to 82% for napin. Napin was positively correlated with glucosinolate (rS = .52**) and seed protein content (rS = .48**). Additional plant material with much larger trait variation was included to develop near‐infrared reflectance spectroscopical calibrations. The Near‐infrared reflectance spectroscopy (NIRS) equations showed high fractions of explained variance in cross and independent validation of around .9 for all traits, indicating that the NIRS equations can be applied in routine screening of plant material.

Carbohydrate metabolism enzymes and phenotypic characterization of diverse lines of the climate‐resilient food, feed, and bioenergy crop Camelina sativa

“…In PCA including enzymatic and agronomic data, yield, harvest index (HI) and seed oil content clustered together and were negatively correlated with dry matter biomass formation, height, onset of flowering and seed protein content. These correlations are in line with that of other crops including Brassica napus (Chang et al, 2022;Marjanović-Jeromela et al, 2011;Stolte et al, 2022) and the negative correlation between seed oil content and seed protein content reported for camelina (Vollmann et al, 2005). It is worth noting that the correlations observed were not only evident in developing seeds but also in leaves, underpinning that physiological process throughout development influence agronomic characters.…”

Genotypic Variations of Morpho-physiological Parameters of Rapeseed (Brassica rapa var. Toria) under Rainfed Condition of Assam

“…Significantly higher protein content was recorded in the cultivar Valesca (21.54%) and lowest in Express (18.19%). Stolte et al (2022) also reported genetic variation for seed storage protein in modern winter rapeseed cultivars (Brassica napus), which varied in the range of 17.5-18.3%.…”