Genetic variation in captive population of chinese alligator, Alligator sinensis , revealed by random amplified polymorphic DNA (RAPD)

Wu, Xiao; Wang, Yi Quan; Zhou, Kai; Zhu, Wei; Nie, Ji; Wang, Chao Lin; Xie, Wan Shu

doi:10.1016/s0006-3207(01)00249-x

Cited by 23 publications

(24 citation statements)

References 29 publications

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“…This may be attributed to the different ability of DNA and biochemical markers to detect polymorphisms. Using RAPD markers on agarose gels, Wu et al (2002) found 3.11 and 10.88% polymorphism in two subpopulations of Alligator sinensis. The genetic distance between the two subpopulations was only of 0.0009.…”

Section: Discussionmentioning

confidence: 96%

“…There is scarce information about RAPD markers in this reptile order (Glenn et al, '98;Wu et al, 2002) and no previous RAPD data in C. latirostris. Recently, population studies on C. latirostris were conducted in Brazil Villela, 2004) by means of specific microsatellite primers developed for Zucoloto et al (2002) that allowed detecting seven polymorphic loci in C. latirostris (Zucoloto et al, 2006).…”

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confidence: 99%

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Genetic and morphometric variability in Caiman latirostris (broad‐snouted caiman), Reptilia, Alligatoridae

et al. 2009

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Caiman latirostris (broad-snouted caiman) is a crocodilian species from Argentina subject of management plans. The goal of this study was estimating the distribution of genetic variability using RAPD markers and quantitative traits in wild populations of C. latirostris from Santa Fe province, Argentina. We sampled animals from four populations to obtain DNA and morphometric measurements. Eight RAPD primers were used and PCR products were analyzed on 4% polyacrylamide gels stained with silver nitrate. Eleven allometric measurements were obtained in animals within 48 hr after birth. We were able to reveal a relatively high number of variable markers in the studied populations. Our estimates of polymorphism and heterozygosity are higher than recorded values in other crocodilians using isozymes, the studied populations showed low levels of gene flow and some population subdivision. The study of quantitative traits conducted by nested analysis of variance and principal component analysis indicated higher levels of variance among nests within populations than among populations. We found that some head measurements have the highest contribution to morphological differences among populations; this fact could support the role of these traits in reproductive or feeding behavior. Estimated genetic differentiation value (F(ST)) among populations was higher than quantitative trait differentiation value (Q(ST)), suggesting a higher contribution of neutral than adaptive loci to the genetic differentiation among populations. Quantitative traits are probably more related with fitness and the differentiation among populations remained relatively lower. The high heritability estimated for some traits indicates great potential to improve them in management plans.

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Section: Discussionmentioning

confidence: 96%

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confidence: 99%

Genetic and morphometric variability in Caiman latirostris (broad‐snouted caiman), Reptilia, Alligatoridae

et al. 2009

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“…Random Amplified Polymorphic DNA (RAPD) is one class of DNA markers used to study the structure of genetic diversity in many species (Wu et al, 2002;Almeida et al, 2003;Telles et al, 2006). This molecular marker can be used for rapid testing, due to its relatively low cost per reaction, the small amounts of DNA needed and the simple method of acquiring data on variation in genomic DNA (Welsh and McClelland, 1990;Hadrys et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil

et al. 2007

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This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An analysis of molecular variance (AMOVA) was performed on the random amplified polymorphic DNA (RAPD) haplotypes. The genetic distances obtained by calculating pairwise Φst among local samples were used to determine population relationships using the unweighted pair-group method (UPGMA) and non-metric multidimensional scaling (NMDS). The cophenetic correlation was calculated to confirm agreement between the genetic matrix and the unweighted pair group method with averages (UPGMA) dendrogram. To determine if genetic distances were correlated to geographical distances we constructed pairwise genetic distance and geographical distance matrices and compared them using the Mantel test. The AMOVA results indicated significant genetic differences (p < 0.001) between E. nattereri populations, representing 69.5% of the within population genetic diversity. The Mantel test showed no significant correlation (r = 0.03; p = 0.45) between the genetic and geographical distance matrices. Our findings indicate that the genetic variation of E. nattereri populations was randomly distributed in geographic space and that gene flow for this species is probably structured at spatial scales smaller than those between our samples

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“…Because the pedigree information of alligators is unclear in ARCCAR, however, the genetic structure of captive populations of Chinese alligators has not been clarified entirely. Besides random amplified polymorphic DNA (RAPD) markers reported by Wu et al [2002], few previous researches had been reported so far on the genetic structure of captive Chinese alligators. Therefore, it is necessary to analyses the genetic structure of captive alligators with more molecular markers.…”

Section: Introductionmentioning

confidence: 99%

AFLP Analysis of genetic variation on captive-bred chinese alligators: an application to select individuals for release

Yan

Wang

et al. 2006

Zoo Biol.

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The Chinese alligator (Alligator sinensis) is a critically endangered species in China. Wild populations of Chinese alligator are on the edge of extinction. Through a release program, some captive-bred alligators will be selected and released into the wild to supplement and renew natural populations. The purpose of this study was to investigate the genetic variation of captive-bred Chinese alligators by AFLP markers and to select individuals with maximally different genetic backgrounds for release. Forty-three captive-bred alligators of the second filial generation from the Anhui Research Center for Chinese Alligator Reproduction (ARCCAR) were surveyed using four primer combinations, yielding 117 AFLP markers. According to AFLP fingerprints, six samples had distinctly different band patterns compared to other samples. When the six samples were removed from the analysis, there were 19 monomorphic loci and 98 polymorphic loci yielding 84% polymorphic loci. Moreover, the genetic similarity (GS) among 37 samples varied from 0.13-0.97, and the average was 0.750370.0064 standard error (SE). When the six samples were included, the GS value among the 43 samples declined and varied from 0.06-0.97, and the average was 0.652370.0079 SE. Based on a cluster analysis using UPGMA, a dendrogram of the 43 alligators was constructed. According to the cluster analysis and gender of the 43 samples, eight Chinese alligators with very different genetic backgrounds were selected and suggested for release with two groups in the future.

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Genetic variation in captive population of chinese alligator, Alligator sinensis , revealed by random amplified polymorphic DNA (RAPD)

Cited by 23 publications

References 29 publications

Genetic and morphometric variability in Caiman latirostris (broad‐snouted caiman), Reptilia, Alligatoridae

Genetic and morphometric variability in Caiman latirostris (broad‐snouted caiman), Reptilia, Alligatoridae

Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil

AFLP Analysis of genetic variation on captive-bred chinese alligators: an application to select individuals for release

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