Genetic variation in three Chinese peat mosses (Sphagnum) based on microsatellite markers, with primer information and analysis of ascertainment bias

Shaw, A. Jonathan; Cao, Tong; Wang, Lisong; Flatberg, Kjell Ivar; Flatberg, Bergfrid; Shaw, Blanka; Zhou, Ping; Boles, Sandra B.; Terracciano, Stefano

doi:10.1639/0007-2745(2008)111[271:gvitcp]2.0.co;2

Cited by 68 publications

(83 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…omegabiotek.com) according to the manufacturer's protocols. Individuals were genotyped at 12 microsatellite loci previously described for Sphagnum (Shaw et al, 2008b), that is, markers 4, 9, 10, 14, 17, 18, 19, 20, 22, 28, 29 and 30. Vouchers for each population, including the whole samples and the remaining part of the plants from which the DNA is extracted from are deposited at the herbarium in Trondheim (TRH).…”

Section: Molecular Analysesmentioning

confidence: 99%

The narrow endemic Norwegian peat moss Sphagnum troendelagicum originated before the last glacial maximum

et al. 2010

View full text Add to dashboard Cite

It is commonly found that individual hybrid, polyploid species originate recurrently and that many polyploid species originated relatively recently. It has been previously hypothesized that the extremely rare allopolyploid peat moss Sphagnum troendelagicum has originated multiple times, possibly after the last glacial maximum in Scandinavia. This conclusion was based on low linkage disequilibrium in anonymous genetic markers within natural populations, in which sexual reproduction has never been observed. Here we employ microsatellite markers and chloroplast DNA (cpDNA)-encoded trnG sequence data to test hypotheses concerning the origin and evolution of this species. We find that S. tenellum is the maternal progenitor and S. balticum is the paternal progenitor of S. troendelagicum. Using various Bayesian approaches, we estimate that S. troendelagicum originated before the Holocene but not before c. 80 000 years ago (median expected time since speciation 40 000 years before present). The observed lack of complete linkage disequilibrium in the genome of this species suggests cryptic sexual reproduction and recombination. Several lines of evidence suggest multiple origins for S. troendelagicum, but a single origin is supported by approximate Bayesian computation analyses. We hypothesize that S. troendelagicum originated in a peat-dominated refugium before last glacial maximum, and subsequently immigrated to central Norway by means of spore flow during the last thousands of years.

show abstract

Section: Molecular Analysesmentioning

confidence: 99%

The narrow endemic Norwegian peat moss Sphagnum troendelagicum originated before the last glacial maximum

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The microsatellite genotyping protocols are described by Ricca et al (2008). The primer sequences for microsatellite amplification are those provided by Shaw et al (2008). The following 18 markers were used in this study : 1, 3, 4, 4long, 7, 9, 10, 12, 14, 17, 18, 19, 20, 22, 26, 28, 29 and 30.…”

Section: Molecular Methodsmentioning

confidence: 99%

Microsatellite variation in three calcium-tolerant species of peat moss detected specific genotypes of Sphagnum warnstorfii on magnesium-rich bedrock

Mikulášková¹,

Veleba²,

Šmerda³

et al. 2017

Preslia

View full text Add to dashboard Cite

Peat mosses are a key functional group in peatlands, driving biogeochemical cycles, habitat development and changes in species composition. They are generally intolerant of calcium and magnesium bicarbonate, but some species are adapted to mineral-rich fens. A previous study found a coincidence between genetic variation and the ability to tolerate high pH/calcium levels in Sphagnum warnstorfii. Here we compare its microsatellite variation with that of two rarer calcium-tolerant species (Sphagnum subnitens, S. contortum), using a novel data set from Eurasia. Because physiological experiments indicate that S. warnstorfii can tolerate high magnesium levels, we included also samples from dolomite and serpentinite. Genetic diversity of S. warnstorfii was higher than that of other species. The Bayesian analysis in program Structure resulted in two population groups of S. warnstorfii. One group coincided with dolomite (Italy, Austria, Estonia) and moderately magnesium-rich (but calcium-poor) rocks (serpentinite, metadolerite, cordieritebearing migmatite on the Bohemian Massif), while the second one coincided with magnesiumpoor bedrock across Eurasia. The principal coordinate analysis revealed a cline between populations from magnesium-rich and magnesium-poor bedrocks, with populations from dolomite and serpentinite forming one extreme. Populations from magnesium-poor bedrock located far from any dolomite or serpentinite formed the opposite extreme of the cline. We demonstrate for the first time that magnesium toxicity may drive bryophyte microevolution, as has repeatedly been shown for vascular plants, including ferns. K e y w o r d s: Bryophyta,

show abstract

“…All specimens are listed in Table 1. Plants were genotyped for microsatellite markers: 1,12,7,17,22,65,68 and 78 (names follow Shaw et al 2008, Stenøien et al 2011. DNA extraction, amplification in multiplexed PCR, and genotyping follow Kyrkjeeide et al (2016).…”

Section: Molecular Analysesmentioning

confidence: 99%

Sphagnum incundum a new species in Sphagnum subg. Acutifolia (Sphagnaceae) from boreal and arctic regions of North America

et al. 2018

Self Cite

View full text Add to dashboard Cite

We describe Sphagnum incundum in Sphagnum subg. Acutifolia (Sphagnaceae, Bryophyta). We used both molecular and morphological methods to describe the new species. Molecular relationships with closely related species were explored based on microsatellites and nuclear and plastid DNA sequences. The morphological description is based on qualitative examination of morphological characters and measurements of leaves and hyalocysts. Morphological characters are compared between closely related species. The results from Feulgen densitometry and microsatellite analysis show that S. incundum is gametophytically haploid. Molecular analyses show that it is a close relative to S. flavicomans, S. subfulvum and S. subnitens, but differs both genetically and in morphological key characters, justifying the description of S. incundum as a new species. The new peatmoss is found in North America along the western coast of Greenland, in Canada from Quebec and Northwest Territories, and Alaska (United States). The new species has a boreal to arctic distribution.

show abstract

Genetic variation in three Chinese peat mosses (Sphagnum) based on microsatellite markers, with primer information and analysis of ascertainment bias

Cited by 68 publications

References 23 publications

The narrow endemic Norwegian peat moss Sphagnum troendelagicum originated before the last glacial maximum

The narrow endemic Norwegian peat moss Sphagnum troendelagicum originated before the last glacial maximum

Microsatellite variation in three calcium-tolerant species of peat moss detected specific genotypes of Sphagnum warnstorfii on magnesium-rich bedrock

Sphagnum incundum a new species in Sphagnum subg. Acutifolia (Sphagnaceae) from boreal and arctic regions of North America

Contact Info

Product

Resources

About