Genetic variation reveals the enhanced microbial hyaluronan biosynthesis via atmospheric and room temperature plasma

Yao, Zhi-Yuan; Gong, Jin‐Song; Liu, Yuru; Jiang, Jiayu; Zhang, Yue-Sheng; Su, Chang; Li, Heng; Kang, Chuan-Li; Liu, Lei; Xu, Zhenghong; Shi, Jin‐Song

doi:10.1016/j.carbpol.2023.120809

Cited by 9 publications

(4 citation statements)

References 32 publications

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Section: Resultsmentioning

confidence: 99%

“…Notably, after data quality control, the Q30 values of all sequencing data met the criteria (>90%) and the total mapping rates of the reference genomes were higher than 95% (Table S2). This result indicated that the quality of the sequencing data reached a qualified level and provided a reliable basis for subsequent analyses [28,29]. Subsequently, we further used the difference analysis mediated by DESeq2 [18] to define significant DEGs according to the rule of Padj value ≤ 0.05, |Log2FC| ≥ 1 [30].…”

Section: Transcriptome Assembly and Difference Analysismentioning

confidence: 86%

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Revealing the Mechanism of Aroma Production Driven by High Salt Stress in Trichomonascus ciferrii WLW

Xian,

Yang,

et al. 2024

Foods

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Douchi is a Chinese traditional fermented food with a unique flavor. Methyl anthranilate (MA) plays an important role in formation of this flavor. However, the complicated relationship between the MA formation and the metabolic mechanism of the key functional microorganisms remains unclear. Here, we elucidated the response mechanism of aroma production driven by high salt stress in Trichomonascus ciferrii WLW (T. ciferrii WLW), which originates from the douchi fermentation process. The highest production of MA was obtained in a 10% NaCl environment. The enhanced expression of the key enzyme genes of the pentose phosphate pathway and shikimic acid pathway directed carbon flow toward aromatic amino acid synthesis and helped sustain an increased expression of metK to synthesize a large amount of the methyl donor S-adenosylmethionine, which promoted methyl anthranilate yield. This provides a theoretical basis for in-depth research on the applications of the flavor formation mechanisms of fermented foods.

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Section: Resultsmentioning

confidence: 99%

Section: Transcriptome Assembly and Difference Analysismentioning

confidence: 86%

Revealing the Mechanism of Aroma Production Driven by High Salt Stress in Trichomonascus ciferrii WLW

Xian,

Yang,

et al. 2024

Foods

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“…This increased the specific growth rate of the mutant and consumed 40 g/L glucose to produce 27.9 g/L succinate with a glucose utilization of 0.54 g/L h and succinate productivity of 0.38 g/L h, a 2.5‐fold increase. After several rounds of ARTP mutagenesis, Yao et al, 136 found that the mutagenesis mechanism was to enhance HA biosynthesis‐related genes and weaken UDP‐Glc NAc downstream genes by regulating the direction of hyaluronic acid (HA) biosynthesis metabolic flow in the strain. This resulted in a 42.9% increase in HA production in the mutant, reaching 0.813 g L ‐1 and a molecular weight of 0.54 × 10 6 Da.…”

Section: Application Of Artp In the Field Of Mutagenesismentioning

confidence: 99%

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Li,

Shen,

Ding

et al. 2024

Cell Biochemistry & Function

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At present, atmospheric and room‐temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment‐friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional mutagenesis strategies, ARTP is used mainly to change the structure of microbial DNA, enzymes, and proteins through a series of physical, chemical, and electromagnetic effects with the organisms, leading to nucleotide breakage, conversion or inversion, causing various DNA damages, so as to screen out the microbial mutants with better biological characteristics. As a result, in recent years, ARTP mutagenesis and the combination of ARTP with traditional mutagenesis have been widely used in microbiology, showing great potential for application. In this review, the recent progress of ARTP mutagenesis in different application fields and bottlenecks of this technology are systematically summarized, with a view to providing a theoretical basis and technical support for better application. Finally, the outlook of ARTP mutagenesis is presented, and we identify the challenges in the field of microbial mutagenesis by ARTP.

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“…Hyaluronidase is a group of carbohydrate-active enzymes that possess major degradation activity toward hyaluronan (HA) (Yao et al 2023;Zhang et al 2022). Hyaluronidases are widely applied in the food industry, cosmetic industry, and medical field.…”

Section: Introductionmentioning

confidence: 99%

Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris

Zhang,

Gong,

Jiang

et al. 2024

Appl Microbiol Biotechnol

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Hyaluronidases catalyze the degradation of hyaluronan (HA), which is finding rising applications in medicine, cosmetic, and food industries. Recombinant expression of hyaluronidases in microbial hosts has been given special attention as a sustainable way to substitute animal tissue-derived hyaluronidases. In this study, we focused on optimizing the secretion of hyaluronidase from Homo sapiens in Pichia pastoris by secretion pathway engineering. The recombinant hyaluronidase was first expressed under the control of a constitutive promoter P GCW14 . Then, two endoplasmic reticulum-related secretory pathways were engineered to improve the secretion capability of the recombinant strain. Signal peptide optimization suggested redirecting the protein into co-translational translocation using the ost1-proα signal sequence improved the secretion level by 20%. Enhancing the co-translational translocation by overexpressing signal recognition particle components further enhanced the secretory capability by 48%. Then, activating the unfolded protein response by overexpressing a transcriptional factor ScHac1p led to a secreted hyaluronidase activity of 4.06 U/mL, which was 2.1-fold higher than the original strain. Finally, fed-batch fermentation elevated the production to 19.82 U/mL. The combined engineering strategy described here could be applied to enhance the secretion capability of other proteins in yeast hosts. Key points• Improving protein secretion by enhancing co-translational translocation in P. pastoris was reported for the first time.• Overexpressing Hac1p homologous from different origins improved the rhPH-20 secretion.• A 4.9-fold increase in rhPH-20 secretion was achieved after fermentation optimization and fed-batch fermentation.

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Genetic variation reveals the enhanced microbial hyaluronan biosynthesis via atmospheric and room temperature plasma

Cited by 9 publications

References 32 publications

Revealing the Mechanism of Aroma Production Driven by High Salt Stress in Trichomonascus ciferrii WLW

Revealing the Mechanism of Aroma Production Driven by High Salt Stress in Trichomonascus ciferrii WLW

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris

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