Genetic variations and haplotypic diversity in the Myostatin gene of New Zealand cattle breeds

Abstract

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Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

“…All seven of the nucleotide substitutions identified in this study have been previously reported in a study of NZ cattle breeds, which included; Hereford, Angus, Shorthorn, Charolais, Red Poll, South Devon, Simmental, Murray Grey, HF × J cross cattle, and some composite breeds [19]. The nucleotide substitutions c.373+751G/T, c.373+803T/G, c.373+877A/G, c.373+895G/C, and c.374−909C/T were identified in all the ten aforementioned breeds, while c.374−842G/C was found in all but four breeds (Red Poll, Shorthorn, Simmental, and Composites breeds) and the c.374−812A/G was only found in Shorthorn and HF × J cross breeds.…”

“…The PCR-SSCP analyses coupled with DNA sequencing revealed five banding patterns (A-E) in the region of intron 1 investigated (Figure 1). A total of seven single-nucleotide substitutions (c.373+751G/T, c.373+803T/G, c.373+877A/G, c.373+895G/C, c.374−909C/T, c.374−842G/C, c.374−812A/G) were identified, all of which have been previously reported [19].…”

“…The intron 1 region of MSTN was amplified using forward and reverse primers (5 -catggtactattgttgagag-3 and 5 -aaggcaaatctattccagg-3 respectively) adapted from the work of Haruna et al [19]. The 15-µL reactions contained the purified DNA on a 1.2-mm diameter disc of the FTA™ card, and a content of 0.25 µM for each primer, 150 µM for each dNTP (Eppendorf, Hamburg, Germany), 3.0 mM Mg 2+ , 0.5 U of Taq DNA polymerase (Qiagen, Hilden, Germany), and 1× the buffer supplied with the DNA polymerase enzyme.…”

Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations

“…Unfortunately, the primers used in this study did not cover these loci. Haruna et al [14] found seven nucleotide variations (c.373+751G>T, c.373+803 T>G, c.373+877A>G, c.373+895G>C, c.374-909C>T, c.374-842G>C, c.374-812A>G) in intron 1 of MSTN in New Zealand cattle breeds, of which two variants (c.748-281C>G and c.748-352C>T) have been reported in Qinchuan and Red Angus cattle [11]. In contrast to the results of this study, Haruna et al [14] did not observe any nucleotide variation in exon 1 of the MSTN gene in New Zealand cattle.…”

“…Mutations in the MSTN gene can affect the proper regulation of skeletal muscle mass, which is important for improved meat production traits. Many previous studies have investigated genetic variations of the MSTN gene not only in cattle [3,6,[10][11][12][13][14], but also in other livestock species, including goats [15], geese [16], chickens [17], and sheep [18]. Several cattle breeds are characterized by a double-muscling (DBM) phenotype, which increases muscle mass due to loss-of-function mutations in bovine MSTN [3,10,19,20].…”