Genetic Variations and Haplotypic Diversity in the Myostatin Gene of Different Cattle Breeds in Russia

Abstract

The myostatin gene (MSTN) in cattle has a number of polymorphisms associated with increased muscle mass. The aim of the current study was to determine the haplotype frequencies of F94L and nt821(del11) MSTN polymorphisms among cattle bred for meat in Russia, using DNA analysis. Using the earlier created test systems based on the AS-PCR and PCR-RFLP methods, six populations of Aberdeen Angus (n = 684), two populations of Limousin (n = 54), one population of Simmental (n = 55), and one population of Belgian Blue (n = 137) belonging to Russian farms were genotyped on nt821(del11) and F94L MSTN polymorphisms. The animal carriers of the mutant allele of nt821(del11) MSTN associated with the double-muscling genetic defect were found in one Aberdeen Angus population at a frequency of 2.18%, but were not found in the Limousin and Simmental populations. However, 100% of the Belgian Blue population were heterozygous carriers of nt821(del11) MSTN. The frequencies of the A allele F94L MSTN desirable for productivity traits in the Limousin populations were the highest and accounted for 0.97 and 1 in populations one and two, while in the Aberdeen Angus, Simmental, and Belgian Blue populations, these figures were considerably lower at 0.04–0.08, depending on the population. The obtained data show the high genetic potential of Russian beef cattle, and facilitate an improvement in meat productivity by preserving the health of animals.

Associations between polymorphisms in the myostatin gene with calving difficulty and carcass merit in cattle

“…While the nt821 deletion is almost fixed in the Belgian Blue population ( Smith et al, 2000 ; Konovalova et al, 2021a ), it has been introduced in other breeds at a low frequency ( Gill et al, 2009 ; Konovalova et al, 2021b ). Because the haplotype with the nt821 deletion and the nt374_51 mutation was detected at a low frequency in the Angus population but was common in the Belgian Blue population, it is likely that the nt821 deletion was introduced into the Angus population through crossbreeding prior to 2003; the oldest Angus animal in the present study with that haplotype was born in 2003.…”

Polymorphism of the myostatin gene exon 1 using PCR-RFLP technique in five beef cattle in Indonesia

“…Its codes region spans 1,128 nucleotides, resulting in a protein length of 375 amino acids [10]. Among these genes, the myostatin gene regulates muscle development and growth traits [11]. Previous studies have identified two mutations in the MSTN gene on exon 1, namely SNPc.111G>C (rs523392653) and SNP.267G>A (rs383271508) [10].…”

The Effect of MSTN Mutation on Bile Acid Metabolism and Lipid Metabolism in Cattle

“…MSTN deactivation by genetic deletion or natural mutation causes muscle hypertrophy, which is widely reported in a variety of mammals [ 2 , 3 , 4 , 5 ]. In cattle breeding, MSTN has been a major subject research topic as a potential target for improving growth and production traits [ 6 , 7 , 8 ]. Using biological breeding technology, we conducted site-specific gene editing inactivation of MSTN by CRISPR/Cas9, effectively reduced the expression of MSTN, produced the purebred MSTN knockout Luxi cattle, which stably inherited the double muscle phenotype caused by MSTN deletion, forming a new breeding strain [ 9 ].…”