Genetic Variations and mRNA Expression of Goat DNAH1 and Their Associations with Litter Size

Wang, Zhen; Wang, Ruolan; Pan, Chuanying; Chen, Hong; Liu, Qu; Wu, Lian; Guo, Zhang; Zhu, Haijing; Lan, Xianyong

doi:10.3390/cells11081371

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…qPCR was performed using SYBR Green Real-time PCR Master Mix (TaKaRa, China). 2 −ΔΔCt method and Student's t tests were used for comparative analysis of gene differential expression ( Wang et al, 2022a , b ). p < 0.05 and p < 0.01 were considered significant and highly significant, respectively.…”

Section: Methodsmentioning

confidence: 99%

Unique feather color characteristics and transcriptome analysis of hair follicles in Liancheng White ducks

Wang,

Guo,

Mou

et al. 2024

Poultry Science

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Section: Methodsmentioning

confidence: 99%

Unique feather color characteristics and transcriptome analysis of hair follicles in Liancheng White ducks

Wang,

Guo,

Mou

et al. 2024

Poultry Science

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“…Reproductive performance directly affects the economic benefits of goat farming, and the ovary, as an organ for oocyte production and reproductive hormone secretion, is directly related to litter size traits [ 2 , 3 ]. Existing studies have identified several genes associated with litter size traits in goats, such as DNAH1 [ 4 ], CFAP43 [ 5 ], CTSS [ 6 ], EPHA6 [ 7 ], and POU1F1 [ 8 ]. However, the key molecular regulatory mechanisms remain unclear.DNA methylation (DNAm) is a type of epigenetic modification that modulates gene expression.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide DNA Methylation Analysis and Functional Validation of Litter Size Traits in Jining Grey Goats

Yang,

He,

Mao

et al. 2024

Genes

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DNA methylation (DNAm) is associated with the reproductive system. However, the genetic mechanism through which DNAm regulates gene expression and thus affects litter size in goats is unclear. Therefore, in the present work, genome-wide DNAm profiles of HP and LP Jining Grey goat ovary tissues were comprehensively analyzed via WGBS, and RNA-Seq data were combined to identify candidate genes associated with litter size traits in the Jining Grey goat. Finally, BSP and RT-qPCR were used to verify the sequencing results of the key genes. Notably, the DNMT genes were downregulated at the expression level in the HP group. Both groups exhibited comparable levels of methylation. A total of 976 differentially methylated regions (DMRs) (973 DMRs for CG and 3 DMRs for CHG) and 310 differentially methylated genes (DMGs) were identified in this study. Through integration of WGBS and RNA-Seq data, we identified 59 differentially methylated and differentially expressed genes (DEGs) and ultimately screened 8 key DMGs (9 DMRS) associated with litter size traits in Jining Grey goats (SERPINB2: chr24_62258801_62259000, NDRG4: chr18_27599201_27599400, CFAP43: chr26_27046601_27046800, LRP1B. chr2_79720201_79720400, EPHA6: chr1_40088601_40088800, TTC29: chr17_59385801_59386000, PDE11A: chr2_117418601_117418800 and PGF: chr10_ 16913801_16914000 and chr10_16916401_16916600). In summary, our research comprehensively analyzed the genome-wide DNAm profiles of HP and LP Jining Grey goat ovary tissues. The data findings suggest that DNAm in goat ovaries may play an important role in determining litter size.

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“…However, due to the complexity and low heritability of this trait, it is difficult to increase the kidding number in a short period of time through traditional breeding methods. With recent technological advances, single nucleotide polymorphisms (SNPs) can be rapidly identified used as a marker in the construction of high-density genetic maps, precise localization of genes, and analysis of population genetic structure; moreover, SNPs have been used as effective molecular markers in goats and other species (Wang et al, 2022). Thus, these advances have clearly and unambiguously revealed the genetic structure of complex traits.…”

Section: Introductionmentioning

confidence: 99%

ALAS1 associated with goat kidding number trait was regulated by the transcription factor ASCL2 to affect granulosa cell proliferation

et al. 2023

Animal Genetics

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ALAS1 is a member of the α‐oxoamine synthase family, which is the first rate‐limiting enzyme for heme synthesis and is important for maintaining intracellular heme levels. In the ovary, ALAS1 is associated with the regulation of ovulation‐related mitochondrial P450 cytochromes, steroid metabolism, and steroid hormone production. However, there are few studies on the relationship between ALAS1 and reproductive traits in goats. In this study, a mutation located in the promoter region of ALAS1 (g.48791372C>A) was found to be significantly (p < 0.05) associated with the kidding number of Yunshang black goats. Specifically, the mean kidding number in the first three litters and the kidding numbers of all three litters were significantly (p < 0.05) higher in individuals with the CA genotype or AA genotype than in those with the CC genotype. To further investigate the regulatory mechanism of ALAS1, the expression of ALAS1 in goat ovarian tissues with different genotypes was verified by real‐time quantitative PCR. The results showed that the expression of ALAS1 was significantly higher in the ovaries of individuals with AA genotype than those with AC and CC genotypes (p < 0.01), and the expression trend of transcription factor ASCL2 was consistent with ALAS1. Additionally, the ALAS1 g.48791372C>A mutation created a new binding site for the transcription factor ASCL2. The luciferase activity assay indicated that the mutation increased the promoter activity of ALAS1. Overexpression of the transcription factor ASCL2 induced increased expression of ALAS1 in goat granulosa cells (p < 0.05). The opposite trend was shown for the inhibition of ASCL2 expression. The results of real‐time quantitative PCR, EdU and Cell Counting Kit‐8 assays indicated that the transcription factor ASCL2 increased the proliferation of goat granulosa cells by mediating the expression of ALAS1. In conclusion, the transcription factor ASCL2 positively regulated the transcriptional activity and expression levels of ALAS1, altering granulosa cell proliferation and the kidding number in goats.

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Genetic Variations and mRNA Expression of Goat DNAH1 and Their Associations with Litter Size

Cited by 10 publications

References 41 publications

Unique feather color characteristics and transcriptome analysis of hair follicles in Liancheng White ducks

Unique feather color characteristics and transcriptome analysis of hair follicles in Liancheng White ducks

Genome-Wide DNA Methylation Analysis and Functional Validation of Litter Size Traits in Jining Grey Goats

ALAS1 associated with goat kidding number trait was regulated by the transcription factor ASCL2 to affect granulosa cell proliferation

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