Genetic variations of Chrysomya megacephala populations in Malaysia (Diptera: Calliphoridae)

Chong, Yi-Vern; Chua, Tock Hing; Song, Beng Kah

doi:10.4236/ae.2014.21009

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…Relevant population genetic markers for C. megacephala have been explored. COI , COII and ISSR markers differentiated between eastern and western populations of C. megacephala in Malaysia [ 169 ], where orographic barriers occur. This study found less genetic variation between populations than expected (relative to related blow flies), and suggested that it was due to urbanization and intercity transport of flies by human activities [ 169 ].…”

Section: Forensic Significancementioning

confidence: 99%

“…COI , COII and ISSR markers differentiated between eastern and western populations of C. megacephala in Malaysia [ 169 ], where orographic barriers occur. This study found less genetic variation between populations than expected (relative to related blow flies), and suggested that it was due to urbanization and intercity transport of flies by human activities [ 169 ]. Amplified fragment length polymorphisms of C. megacephala sampled across Florida and Alabama indicated some spatial differentiation, but also that its genetic diversity was diminished relative to both populations in its native range and populations of native blow flies in the USA [ 59 ].…”

Section: Forensic Significancementioning

confidence: 99%

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The uses of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) in forensic entomology

Badenhorst

Villet

2018

Forensic Sciences Research

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Chrysomya megacephala (Fabricius, 1794) occurs on every continent and is closely associated with carrion and decaying material in human environments. Its abilities to find dead bodies and carry pathogens give it a prominence in human affairs that may involve prosecution or litigation, and therefore forensic entomologists. The identification, geographical distribution and biology of the species are reviewed to provide a background for approaches that four branches of forensic entomology (urban, stored-product, medico-criminal and environmental) might take to investigations involving this fly.

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Section: Forensic Significancementioning

confidence: 99%

Section: Forensic Significancementioning

confidence: 99%

The uses of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) in forensic entomology

Badenhorst

Villet

2018

Forensic Sciences Research

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“…megacephala in Malaysia (5 locations) using COI. Neighbour joining tree based on COI sequence showed two main groups, one branch comprised Penang and Selangor populations while another consisted of Johor, Pahang, and Sabah populations [54]. Including more genes in such analyses could be promising.…”

Section: Discussionmentioning

confidence: 99%

A molecular, morphological, and physiological comparison of English and German populations of Calliphora vicina (Diptera: Calliphoridae)

et al. 2018

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The bluebottle blow fly Calliphora vicina is a common species distributed throughout Europe that can play an important role as forensic evidence in crime investigations. Developmental rates of C. vicina from distinct populations from Germany and England were compared under different temperature regimes to explore the use of growth data from different geographical regions for local case work. Wing morphometrics and molecular analysis between these populations were also studied as indicators for biological differences. One colony each of German and English C. vicina were cultured at the Institute of Legal Medicine in Frankfurt, Germany. Three different temperature regimes were applied, two constant (16°C & 25°C) and one variable (17–26°C, room temperature = RT). At seven time points (600, 850, 1200, 1450, 1800, 2050, and 2400 accumulated degree hours), larval lengths were measured; additionally, the durations of the post feeding stage and intrapuparial metamorphosis were recorded. For the morphometric and molecular study, 184 females and 133 males from each C. vicina population (Germany n = 3, England n = 4) were sampled. Right wings were measured based on 19 landmarks and analyzed using canonical variates analysis and discriminant function analysis. DNA was isolated from three legs per specimen (n = 61) using 5% chelex. A 784 bp long fragment of the mitochondrial cytochrome b gene was sequenced; sequences were aligned and phylogenetically analyzed. Similar larval growth rates of C. vicina were found from different geographic populations at different temperatures during the major part of development. Nevertheless, because minor differences were found a wider range of temperatures and sampling more time points should be analyzed to obtain more information relevant for forensic case work. Wing shape variation showed a difference between the German and English populations (P<0.0001). However, separation between the seven German and English populations at the smaller geographic scale remained ambiguous. Molecular phylogenetic analysis by maximum likelihood method could not unambiguously separate the different geographic populations at a national (Germany vs England) or local level.

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“…Research by Chong et al (2014) suggested that since the Chrysomya genomes tend to be highly repetitive, using the repeat motif as the priming region could create reliable polymorphic loci. These markers would again only be counted if the resulting amplicon was longer than 50 and shorter than 500 base pairs long.…”

Section: Clc Genomics Workhopmentioning

confidence: 99%

“…Using published information on insect microsatellites, I searched for the various motifs that have been successful with C. megacephala as well as other members of the family Calliphoridae (Florin & Gyllenstrand, 2002;He et al, 2007;Torres et al, 2008;Chong et al, 2014;Farncombe et al, 2014). The CLC also uses Primer 3 parameters to determine possible primer pairs, their annealing temperatures, GC content, and self-complementarity.…”

Section: Clc Genomics Workhopmentioning

confidence: 99%

Employing Limited Next Generation Sequence Data for the Development of Genetic Loci of Phylogenetic and Population Genetic Utility

Evenstone¹

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Genetic variations of Chrysomya megacephala populations in Malaysia (Diptera: Calliphoridae)

Abstract: ABSTRACT

Cited by 8 publications

References 32 publications

The uses of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) in forensic entomology

The uses of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) in forensic entomology

A molecular, morphological, and physiological comparison of English and German populations of Calliphora vicina (Diptera: Calliphoridae)

Employing Limited Next Generation Sequence Data for the Development of Genetic Loci of Phylogenetic and Population Genetic Utility

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