Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1

Popescu, L; Gaudreault, Natasha N.; Whitworth, Kristen M.; Murgia, Maria V.; Nietfeld, Jerome C.; Mileham, Alan J.; Samuel, Melissa; Wells, Kevin D.; Prather, Randall S.; Rowland, Raymond R. R.

doi:10.1016/j.virol.2016.11.012

Cited by 78 publications

(52 citation statements)

References 34 publications

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“…These data provide further support for the results presented in Tables 2 and 3 and support previous published reports describing increased numbers of circulating CD163-positive monocytes during acute ASFV infection 25 . Even though CD163 has been proposed as a candidate receptor for ASFV 26 , recent data show that CD163-knockout pigs support GRG infection and exhibit clinical signs consistent with acute infection 27 . The association of DEGs with the immune response was found in the results from the Human Atlas, including DEGs associated with NK and T cell function.…”

Section: Discussionmentioning

confidence: 99%

Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

Jaing

Rowland

Allen

et al. 2017

Sci Rep

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African swine fever virus (ASFV) is a macrophage-tropic virus responsible for ASF, a transboundary disease that threatens swine production world-wide. Since there are no vaccines available to control ASF after an outbreak, obtaining an understanding of the virus-host interaction is important for developing new intervention strategies. In this study, a whole transcriptomic RNA-Seq method was used to characterize differentially expressed genes in pigs infected with a low pathogenic ASFV isolate, OUR T88/3 (OURT), or the highly pathogenic Georgia 2007/1 (GRG). After infection, pigs infected with OURT showed no or few clinical signs; whereas, GRG produced clinical signs consistent with acute ASF. RNA-Seq detected the expression of ASFV genes from the whole blood of the GRG, but not the OURT pigs, consistent with the pathotypes of these strains and the replication of GRG in circulating monocytes. Even though GRG and OURT possess different pathogenic properties, there was significant overlap in the most upregulated host genes. A small number of differentially expressed microRNAs were also detected in GRG and OURT pigs. These data confirm previous studies describing the response of macrophages and lymphocytes to ASFV infection, as well as reveal unique gene pathways upregulated in response to infection with GRG.

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Section: Discussionmentioning

confidence: 99%

Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

Jaing

Rowland

Allen

et al. 2017

Sci Rep

Self Cite

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“…However, the susceptibility of host cells to ASFV seems to be linked to maturity since in vitro maturation of porcine blood monocyte cells (PBMCs) to macrophages, correlating with an up-regulation of CD203 and CD163 expression, has been shown to increase ASFV infection 24 , 43 . Nevertheless, the role of CD163 in ASFV infection is controversial since it has been published that the expression of CD163 alone is not enough to increase the susceptibility to the virus in non-permissive cells 44 , and pigs lacking CD163 showed no resistance to infection with the ASFV isolate Georgia 2007/1 45 .…”

Section: Introductionmentioning

confidence: 99%

Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production

Sánchez

Riera

Nogal

et al. 2017

Sci Rep

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African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, C∆2+ and WSL, were compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibility to ASFV infection and virus production. The virulent ASFV Armenia/07, E70 or the naturally attenuated NHV/P68 strains were used as viral models. Cells expressed only low levels of specific receptors linked to the monocyte/macrophage lineage, with low levels of infection overall, with the exception of WSL, which showed more efficient production of strain NHV/P68 but not of strains E70 and Armenia/07.

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“…receptors) was emphasized by Reiner (2016). This was confirmed by Popescu et al (2017) who reported that genetically edited pigs lacking the virus receptor CD163 for African swine fever died post virus infection.…”

Section: Breeding Strategiesmentioning

confidence: 84%

Invited review: Piglet survival: benefits of the immunocompetence

Heuß

Pröll-Cornelissen

Neuhoff

et al. 2019

Animal

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Piglet mortality has a negative impact on animal welfare and public acceptance. Moreover, the number of weaned piglets per sow mainly determines the profitability of piglet production. Increased litter sizes are associated with lower birth weights and piglet survival. Decreased survival rates and performance of piglets make the control of diseases and infections within pig production even more crucial. Consequently, selection for immunocompetence becomes an important key aspect within modern breeding programmes. However, the phenotypic recording of immune traits is difficult and expensive to realize within farm routines. Even though immune traits show genetic variability, only few examples exist on their respective suitability within a breeding programme and their relationships to economically important production traits. The analysis of immune traits for an evaluation of immunocompetence to gain a generally improved immune response is promising. Generally, in-depth knowledge of the genetic background of the immune system is needed to gain helpful insights about its possible incorporation into breeding programmes. Possible physiological drawbacks for enhanced immunocompetence must be considered with regards to the allocation theory and possible trade-offs between the immune system and performance. This review aims to discuss the relationships between the immunocompetence of the pig, piglet survival as well as the potential of these traits to be included into a breeding strategy for improved robustness.

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Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1

Cited by 78 publications

References 34 publications

Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production

Invited review: Piglet survival: benefits of the immunocompetence

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