Genetically engineered mesenchymal stromal cells in cancer gene therapy

Abstract

Based on our experimental data, we aimed to emphasise the perspectives of the use of mesenchymal stromal cells (MSC) in the cancer gene therapy. On the other hand, we would like to point out factors which should be taken into consideration at their clinical use. In this review we defi ne MSC as unique targets for targeted therapy. We proved the effi cacy of experimental therapeutic approach utilising enzymatic conversion of non-toxic prodrug into chemotherapeutic by engineered MSC, and we observed signifi cant cytotoxic effect in many preclinical models including metastatic disease. Treatment was enabled by affi nity of MSC to tumour tissue and subsequent delivery of therapeutic molecule into the tumour. We also observed decreased effi cacy of cell-mediated gene therapy on chemoresistant tumour cells. Moreover MSC can exert a supportive effect on tumour cells as well as to decrease the effi cacy of conventional treatment. Besides obvious unique benefi ts connected to the use of MSC we pointed also to possible risks associated with their clinical application (Ref. 24). Text in PDF www.elis.sk.

Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study

“…The therapeutic potential of MSCs has been explored in a number of phase I, II, and III clinical trials [23], of which several were targeted against graft versus-host disease and to support engraftment of hematopoietic stem cells [24, 25]. Yet, very few of these trials use MSCs to treat tumor diseases [23, 26, 27], such as gastrointestinal, lung, and ovarian cancer. As for the study targeting ovarian cancer, MSCs expressing cytokines were used as therapeutic payload [23] and were derived from bone marrow.…”

Acetylcholine Receptors in Mesenchymal Stem Cells

“…MSCs tend to differentiate down mesodermal lineages, however, under appropriate stimuli, it has been suggested that MSCs are capable of differentiation into tissues of endodermal and neuroectodermal lineages [42]. As MSCs are self-renewing cells with immunomodulatory properties and the ability to be differentiated into several lineages [46][47][48][49] they are a vital resource for tissue engineering, regenerative medicine, and cell-based therapy research [39,46,50].…”

Enhanced Anticancer Property of Ad-MSC-TRAIL on Breast Cancer Cells When Combined with the Aqueous Extract of Berberis vulgaris

“…In recent studies, gene therapy has been discussed as a potentially promising way to treat cancers, but the problems are how to guide genes to the tumor site(s) or which vehicle can selectively find cancer location and which vehicle can be stable in the tumor microenvironment (3). The ongoing process of research has shown that mesenchymal stem cells can be a noticeable strategy in anticancer therapies (4).…”