Genetically modified cells in regenerative medicine and tissue engineering☆

Sheyn, Dima; Mizrahi, Olga; Benjamin, Shimon; Gazit, Zulma; Pelled, Gadi; Gazit, Dan

doi:10.1016/j.addr.2010.01.002

Cited by 71 publications

(40 citation statements)

References 197 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current treatments are based on transplantation of autologous chondrocytes and osteochondral allografts (Bartlett et al 2005; Sheyn et al 2010; Augustyniak et al 2015). In this regard, pluripotent stem cells have arisen as an attractive cell source for repairing large cartilage defects after the primary surgery of the laryngeal cancer.…”

Section: Cartilagementioning

confidence: 99%

The importance of stem cell engineering in head and neck oncology

et al. 2016

View full text Add to dashboard Cite

Head and neck squamous cell carcinoma is the sixth leading cause of cancer worldwide. The most common risk factors are carcinogens (tobacco, alcohol), and infection of the human papilloma virus. Surgery is still considered as the treatment of choice in case of head and neck cancer, followed by a reconstructive surgery to enhance the quality of life in the patients. However, the widespread use of artificial implants does not provide appropriate physiological activities and often cannot act as a long-term solution for the patients. Here we review the applicability of multiple stem cell types for tissue engineering of cartilage, trachea, vocal folds and nerves for head and neck injuries. The ability of the cells to self-renew and maintain their pluripotency state makes them an attractive tool in tissue engineering.

show abstract

Section: Cartilagementioning

confidence: 99%

The importance of stem cell engineering in head and neck oncology

et al. 2016

View full text Add to dashboard Cite

show abstract

“…One possible route to overcome this issue is through the use of genetically engineered cell therapy, which has become a cutting-edge approach for tissue regeneration, and it has been under intensive experimental evaluation. 196,197 Cell-based gene delivery approaches to induce bone formation through the transfection of cells with BMP-2, 4, 6, 7, and 9 genes have led to superior bone formation in several animal models. [198][199][200][201][202][203] Gene therapy approaches for cartilage regeneration have also been explored with transfection of cells with BMP-2, BMP-7, TGF-b1, IGF-1, among others, acting individually or in synergy.…”

Section: From Single To Multiple Bioactive Factor Delivery For Skeletmentioning

confidence: 99%

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

Santo

Gomes²,

Mano³

et al. 2013

Tissue Engineering Part B: Reviews

105

View full text Add to dashboard Cite

The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of bioactive factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth factors and cytokines involved in each biological response, it is expected that an appropriate combination of bioactive factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple bioactive factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of bioactive factors. A comprehensive collection of studies focused on the delivery of bioactive factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth factors.

show abstract

“…Moreover, due to the extensive process of isolation of growth factors in their purified form for tissue development, subsequently increases its cost. Thus, genetic manipulations of cells to increase the expression of target gene for the high degree of function is becoming an important focus by researchers to establish genetically modified cells with overexpressed gene function for tissue regeneration [115]. For this purpose, the most common approach for enhancing electrostatic interactions between target cells and gene vectors is by means of cationic agents such as cationic liposomes [116,117] and polymers [118].…”

Section: Magnetofectionmentioning

confidence: 99%

Magnetic Nanoparticles in Tissue Regeneration

Tripathi¹,

Melo²,

D’Souza³

2013

Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering

View full text Add to dashboard Cite

The remote control operation and manipulation of cells is the prime advantage of magnetic nanoparticles. Control of specific cellular components in vitro as well as in vivo offers a powerful tool for the understanding of cellular functions and molecular signaling to scientist, which helps to develop precise therapeutic applications by clinicians. Cells surface or intracellular labeling by the use of magnetic nanoparticles as an investigation tool in its preliminary stage are successfully developed for the diagnostic and other biomedical applications. Currently, magnetic nanoparticle applications have prominently shifted their focus towards the engineering of cells and their fate in the determination at a molecular level for three-dimensional tissue regeneration. Magnetic force-assisted tissue engineering is often referred to as a "Mag-TE." This chapter is divided into two main sections. The first section presents an overview about the properties, synthesis and structure of magnetic nanoparticles. The second section focuses on how magnetic nanoparticles have been applied in the cell directed tissue engineering and regenerative medicine with potential future prospects.

show abstract

Genetically modified cells in regenerative medicine and tissue engineering☆

Cited by 71 publications

References 197 publications

The importance of stem cell engineering in head and neck oncology

The importance of stem cell engineering in head and neck oncology

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

Magnetic Nanoparticles in Tissue Regeneration

Contact Info

Product

Resources

About