Geniposide, a novel agonist for GLP-1 receptor, prevents PC12 cells from oxidative damage via MAP kinase pathway

Liu, Jianhui; Yin, Fei; Zheng, Xiangkuo; Jiajia, Jing; Hu, Yinhe

doi:10.1016/j.neuint.2007.04.021

Cited by 141 publications

(110 citation statements)

References 27 publications

Supporting

Mentioning

109

Contrasting

Order By: Relevance

“…The cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as described before. 11,12) To determine whether PI3K signaling pathway is involved in the cytoprotective effect of geniposide, LY294002, a selective inhibitor of PI3K, was used, which was added to cell cultures 30 min before treatment with SIN-1. Control neurons were treated with the appropriate amount of vehicle.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Geniposide Induces the Expression of Heme Oxygenase-1 via PI3K/Nrf2-Signaling to Enhance the Antioxidant Capacity in Primary Hippocampal Neurons

Yin

Liu

Zheng

et al. 2010

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

Oxidative stress in brain is emerging as a potential causal factor in aging and age-related neurodegenerative disorders. A large body of evidence shows that induction of endogenous antioxidative proteins seems to be a reasonable strategy for delaying the progression of cell injury. In this study, geniposide upregulates the expression of heme oxygenase-1 (HO-1) to attenuate the cell apoptosis induced by 3-morpholinosydnonimine hydrochloride (SIN-1) in primary cultured hippocampal neurons. Furthermore, geniposide induces the nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) and activation of phosphatidylinositol 3-kinase (PI3K) in the presence of oxidative stress, and both LY294002 (a specific inhibitor of PI3K) and Zinc protoporphyrin (ZnPP, an inhibitor of HO-1) decrease the cytoprotective action of geniposide in hippocampal neurons. Taken together, the novel cytoprotective mechanism of geniposide to antagonize oxidative stress may be involved in PI3K-and Nrf2-mediated upregulation of the antioxidative enzyme HO-1.

show abstract

Section: Methodsmentioning

confidence: 99%

“…9) Our previous works showed that geniposide was a novel agonist for glucagon-like peptide 1 receptor (GLP-1R), which induced the neuronal differentiation of PC12 cells and prevented PC12 cells from oxidative stress. [10][11][12] However, the mechanisms involved in these cytoprotective effects of geniposide remain to be fully established.…”

mentioning

confidence: 99%

Geniposide Induces the Expression of Heme Oxygenase-1 via PI3K/Nrf2-Signaling to Enhance the Antioxidant Capacity in Primary Hippocampal Neurons

Yin

Liu

Zheng

et al. 2010

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was found that geniposide, with the activation of GLP-1R, induced insulin secretion in a dosedependent manner and showed neurotrophic properties Brought to you by | MIT Libraries Authenticated Download Date | 5/9/18 11:23 PM by stimulating cAMP production. Furthermore, the phosphatidylinositol 3-kinase (PI3K) signaling pathway and the mitogen-activated protein kinases (MAPK) pathway are involved in the neuroprotection of geniposide against oxidative damage in PC12 cells and SH-SY5Y cells (Liu et al, 2007Guo et al, 2012;Sharma et al, 2013). The activities of geniposide in neurons include an increased expression of genes that are linked to cell growth and repair, inhibition of apoptosis, and reduction of inflammatory responses (see Figure 2 for details on the underlying molecular mechanism).…”

Section: Molecular Pathwaysmentioning

confidence: 99%

“…One study found that genipin suppressed apoptosis in cultured cells via the inhibition of caspase activation and mitochondrial function (Yamamoto et al, 2000). Furthermore, strong evidence suggests that geniposide regulates the expression of apoptosis-related proteins via the MAPK signaling pathway, thereby overcoming the toxicity of Aβ (Liu et al, 2007 …”

Section: Possible Neuroprotective Mechanisms In Ad Geniposide Reducesmentioning

confidence: 99%

“…Furthermore, geniposide could increase the expression of HO-1 and resist the oxidative damage induced by H 2 O 2 and 3-morpholinosydnonimine hydrochloride (SIN-1) in PC12 cells by activating the MAPK-p90RSK, PI3K/Akt-Nrf2, and protein kinase A (PKA)-cAMP-response element binding protein signaling pathways (Liu et al, 2007Yin et al, 2010a,b). Pretreatment with geniposide markedly improved the viability of cells and regulated the expression of apoptotic protein involved in mitochondrial-mediated apoptosis in PC12 cells induced by CoCl 2 .…”

Section: Attenuation Of Mitochondrial Oxidative Stress By Geniposidementioning

confidence: 99%

See 1 more Smart Citation

Neuroprotective effects of geniposide on Alzheimer’s disease pathology

Liu

Hölscher³

et al. 2015

Reviews in the Neurosciences

View full text Add to dashboard Cite

AbstractA growing body of evidence has linked two of the most common aged-related diseases: type 2 diabetes mellitus (T2DM) and Alzheimer’s disease (AD). It has led to the notion that drugs developed for the treatment of T2DM may be beneficial in modifying the pathophysiology of AD. As a receptor agonist of glucagon-like peptide-1 (GLP-1R), which is a newer drug class to treat T2DM, geniposide shows clear effects in inhibiting pathological processes underlying AD, such as promoting neurite outgrowth. In the present article, we review the possible molecular mechanisms of geniposide to protect the brain from pathologic damages underlying AD: reducing amyloid plaques, inhibiting τ phosphorylation, preventing memory impairment and loss of synapses, reducing oxidative stress and the chronic inflammatory response, and promoting neurite outgrowth via the GLP-1R signaling pathway. In summary, the Chinese herb geniposide shows great promise as a novel treatment for AD.

show abstract

Simultaneous determination of geniposide and its metabolites genipin and genipinine in culture of Aspergillus niger by HPLC

Chen

Han

Zhang

et al. 2008

Biomedical Chromatography

View full text Add to dashboard Cite

When cultivated with Aspergillus niger, geniposide, an important drug, is transformed into genipin and genipinine. A simple and rapid HPLC method for simultaneous determination of geniposide and its two metabolites in broth of A. niger is described. The chromatographic separation was achieved on a C18 ODS column (250 x 4.6 mm) by gradient elution with 0.1% formic acid in water and 0.1% formic acid in acetonitrile as the gradient mixtures. The flow rate was 1 mL/min, the detection wavelength was 238 nm and the column temperature was kept at 28 degrees C. The retention times of geniposide, genipin and genipinine were 10.9, 13.8 and 21.5 min, respectively. The mean absolute recoveries of three analysts were over 98%. Quantification limits were 0.01 microg/mL for geniposide and 0.02 microg/mL for the two metabolites. The method was applied for the quantification of geniposide, genipin and genipinine during fermentation and the evaluation of the bioavailabilities of these three compounds in Caco-2 monolayer.

show abstract

Geniposide, a novel agonist for GLP-1 receptor, prevents PC12 cells from oxidative damage via MAP kinase pathway

Cited by 141 publications

References 27 publications

Geniposide Induces the Expression of Heme Oxygenase-1 via PI3K/Nrf2-Signaling to Enhance the Antioxidant Capacity in Primary Hippocampal Neurons

Geniposide Induces the Expression of Heme Oxygenase-1 via PI3K/Nrf2-Signaling to Enhance the Antioxidant Capacity in Primary Hippocampal Neurons

Neuroprotective effects of geniposide on Alzheimer’s disease pathology

Simultaneous determination of geniposide and its metabolites genipin and genipinine in culture of Aspergillus niger by HPLC

Contact Info

Product

Resources

About