Genistein inhibits migration and invasion of cervical cancer HeLa cells by regulating FAK-paxillin and MAPK signaling pathways

Chen, Chunlin; Wang, Yanxi; Chen, Si; Ruan, Xiaofeng; Liao, Huiyan; Zhang, Yingxuan; Sun, Jianhua; Gao, Jie; Deng, Gaopi

doi:10.1016/j.tjog.2020.03.012

Cited by 22 publications

(14 citation statements)

References 25 publications

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“…The activation of the FAK-paxillin signaling pathway has been considered a crucial index for tumor metastasis (39). In the current study, carbon ions distinctly limited the area and speed of wound healing cell migration as well as the number of invading cells through the invasion chamber (Figure 3).…”

Section: Discussionmentioning

confidence: 53%

Carbon Ion Irradiation Enhances the Anti-tumor Efficiency in Tongue Squamous Cell Carcinoma via Modulating the FAK Signaling

Qian

Bing

et al. 2021

Front. Public Health

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Oral cancer is a very aggressive disease with high rates of recurrence and metastasis. This study aimed at addressing how efficiently tongue cancer is suppressed after carbon ion irradiation. Here, the close relationship between upregulated expression of focal adhesion kinase (FAK) and high metastatic status in tongue squamous cell carcinoma patients was validated using bioinformatics and immunohistochemical analyses. Our data indicated that FAK suppression significantly enhanced the killing effect induced by irradiation in the tongue cancer cell line CAL27, as evidenced by increased apoptotic induction and reduced colony formation. More importantly, in FAK-deficient cells, carbon ion irradiation was shown to remarkably inhibit migration and invasion by delaying wound healing and slowing down motility. Further studies revealed that irradiation exposure caused disorganization of the actin cytoskeleton and reduced cell adhesive energy in FAK-deficient cells. Moreover, carbon ion treatment, in combination with FAK silencing, markedly blocked the phosphorylation levels of FAK, and paxillin, which partly contributed to the reduced motility of tongue squamous cell carcinoma CAL27 cells. Collectively, these results suggest that the prominent obstructing role of carbon ion irradiation in the growth inhibition and metastatic behavior of tumors, including attenuation of cell adhesiveness, motility, and invasiveness, could be distinctly modulated by FAK-mediated downstream pathways.

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Section: Discussionmentioning

confidence: 53%

Carbon Ion Irradiation Enhances the Anti-tumor Efficiency in Tongue Squamous Cell Carcinoma via Modulating the FAK Signaling

Qian

Bing

et al. 2021

Front. Public Health

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“…In contrast, following treatment with genistein, a significant inhibition of p38 and ERK/MAPK phosphorylation was found, suggesting that this isoflavone has a modulatory effect on this pathway. 106 Colorectal cancer: Qiu et al 107 evaluated the effects of 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone (5HHMF) on human colon cancer cells. It was found that treatment with this flavonoid did not affect the levels of epidermal growth factor receptor (EGFR) in HCT116 cells, the compound inhibited EGFR activity by reducing phosphorylation at Tyr 1068.…”

Section: Flavonoids and The Tgf-β Signalingmentioning

confidence: 99%

The role of flavonoids in the regulation of epithelial‐mesenchymal transition in cancer: A review on targeting signaling pathways and metastasis

Proença

Freitas

Ribeiro

et al. 2023

Medicinal Research Reviews

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One of the hallmarks of cancer is metastasis, a process that entails the spread of cancer cells to distant regions in the body, culminating in tumor formation in secondary organs. Importantly, the proinflammatory environment surrounding cancer cells further contributes to cancer cell transformation and extracellular matrix destruction. During metastasis, front‐rear polarity and emergence of migratory and invasive features are manifestations of epithelial‐mesenchymal transition (EMT). A variety of transcription factors (TFs) are implicated in the execution of EMT, the most prominent belonging to the Snail Family Transcriptional Repressor (SNAI) and Zinc Finger E‐Box Binding Homeobox (ZEB) families of TFs. These TFs are regulated by interaction with specific microRNAs (miRNAs), as miR34 and miR200. Among the several secondary metabolites produced in plants, flavonoids constitute a major group of bioactive molecules, with several described effects including antioxidant, antiinflammatory, antidiabetic, antiobesogenic, and anticancer effects. This review scrutinizes the modulatory role of flavonoids on the activity of SNAI/ZEB TFs and on their regulatory miRNAs, miR‐34, and miR‐200. The modulatory role of flavonoids can attenuate mesenchymal features and stimulate epithelial features, thereby inhibiting and reversing EMT. Moreover, this modulation is concomitant with the attenuation of signaling pathways involved in diverse processes as cell proliferation, cell growth, cell cycle progression, apoptosis inhibition, morphogenesis, cell fate, cell migration, cell polarity, and wound healing. The antimetastatic potential of these versatile compounds is emerging and represents an opportunity for the synthesis of more specific and potent agents.

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“…First, the phosphorylation status of Cx37 in quiescent endothelium in mice and humans (with either polymorphism) is unknown and could differ for the polymorphic forms. Second, the phosphorylation status of hCx37-P319 and hCx37-S319 as expressed in HeLa and SK-Hep-1 cells is unknown and could differ from what occurs in Rin cells, as both HeLa and SK-Hep-1 are metastatic cancer cell lines with likely aberrant growth regulatory signaling [82, 83]. And third, the downstream mediators of Cx37-induced growth arrest in Rin and endothelial cells are unknown and could be dysregulated in HeLa and SK-Hep-1 cells.…”

Section: Phosphorylation Of Cx37 Likely Induces a Closed Channel Conf...mentioning

confidence: 99%

Connexin37 Regulates Cell Cycle in the Vasculature

Fang

Burt

2022

J Vasc Res

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Control of vascular cell growth responses is critical for development and maintenance of a healthy vasculature. Connexins – the proteins comprising gap junction channels – are key regulators of cell growth in diseases such as cancer, but their involvement in controlling cell growth in the vasculature is less well appreciated. Connexin37 (Cx37) is one of four connexin isotypes expressed in the vessel wall. Its primary role in blood vessels relies on its unique ability to transduce flow-sensitive signals into changes in cell cycle status of endothelial (and perhaps, mural) cells. Here, we review available evidence for Cx37’s role in the regulation of vascular growth, vessel organization, and vascular tone in healthy and diseased vasculature. We propose a novel mechanism whereby Cx37 accomplishes this with a phosphorylation-dependent transition between closed (growth-suppressive) and multiple open (growth-permissive) channel conformations that result from interactions of the C-terminus with cell-cycle regulators to limit or support cell cycle progression. Lastly, we discuss Cx37 and its downstream signaling as a novel potential target in the treatment of cardiovascular disease, and we address outstanding research questions that still challenge the development of such therapies.

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Genistein inhibits migration and invasion of cervical cancer HeLa cells by regulating FAK-paxillin and MAPK signaling pathways

Cited by 22 publications

References 25 publications

Carbon Ion Irradiation Enhances the Anti-tumor Efficiency in Tongue Squamous Cell Carcinoma via Modulating the FAK Signaling

Carbon Ion Irradiation Enhances the Anti-tumor Efficiency in Tongue Squamous Cell Carcinoma via Modulating the FAK Signaling

The role of flavonoids in the regulation of epithelial‐mesenchymal transition in cancer: A review on targeting signaling pathways and metastasis

Connexin37 Regulates Cell Cycle in the Vasculature

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