Genital Human Papillomavirus Testing by in Situ Hybridization in Liquid Atypical Cytologic Materials and Follow-up Biopsies

Bewtra, Chhanda; Xiao, Qiang; Soundararajan, Suganthi; Gatalica, Zoran; Hatcher, Lori

doi:10.1159/000326119

Cited by 9 publications

(6 citation statements)

References 5 publications

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“…Hesselink et al 14 We observed a low HPV positive rate (23.5%) using INFORM HPV in women who had ASCUS Pap results. A similar low HPV positive rate (33.0%) in women with ASCUS was also reported in SurePath Pap specimens by Bewtra et al, 11 although no direct comparison data with HC2 were available in both studies. Direct comparison between INFORM HPV and HC2 were reported in ThinPrep Pap specimens in which lower positive rates were also observed using ISH assay compared to those using HC2.…”

Section: Discussionsupporting

confidence: 77%

“…Schiller et al 15 Currently, for the triage of women with ASCUS Pap results, a sensitivity of 95.9-96.3% and an NPV of 98.9-99.5% has been widely accepted as a standard that was established by ALTS clinical trial using HC2 for predicting CIN 2/3. 2 In a recently published study using SurePath as the cytology collection method, Bewtra et al 11 reported that in 100 women with ASCUS and follow-up biopsies, the INFORM HPV correctly predicted CIN 2/3 in 13 of 18 women, for a sensitivity of 72%. Davis-Devine et al 10 compared INFORM HPV with HC2 in SurePath Pap specimens.…”

Section: Discussionmentioning

confidence: 99%

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A Human Papillomavirus Testing System in Women with Abnormal Pap Results

et al. 2007

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

A Human Papillomavirus Testing System in Women with Abnormal Pap Results

et al. 2007

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“…Positive staining was visualized using the precipitating chromogenic reaction NBT/BCIP with a nuclear localization. A positive ISH is defined by a dark blue nuclear stain, in papillary squamous epithelium [10,[13][14][15][16].…”

Section: Methodsmentioning

confidence: 99%

Papillomas of the External Ear Canal: Report of Ten cases in Chinese Patients with HPV In Situ Hybridization

Wang

Yee

Wen

et al. 2009

Head and Neck Pathol

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Squamous papilloma is a benign exophytic proliferation which can occur occasionally in the external ear canal. It is widely assumed that the Human Papilloma Virus (HPV) is an etiologic factor of papillomas. Available techniques for detection of HPV genomes include immunohistochemistry, Southern blot hybridization, in situ hybridization (ISH), and polymerase chain reaction. To our knowledge, HPV typing has not been reported on tissue sections of papillomas in the external ear canal. We report HPV ISH analysis in ten cases of papillomas, involving the external ear canal in Chinese patients. These papilloma excrescences were less than 1 cm in diameter, and were benign morphologically. Automated HPV ISH analysis was performed for the hybridization of DNA probes, including both low-risk and high-risk HPV subtypes. HPV ISH results revealed that seven out of ten cases were positive for low-risk HPV (6, 11), three cases demonstrated no hybridization for low-risk HPV probe, and none of the cases revealed any detection of high-risk HPV (16, 18, 31, 33, 35, 39, 51, 52, 56, 58, and 66). On follow-up after 18-29 months (average 24.5 months), eight patients were doing well, with no local recurrence after excision. Two patients were lost to follow-up. Our results confirm that benign papillomas of the external ear canal are associated with low-risk HPV infection with benign behavior and neither recurrence nor high grade dysplasia.

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“…Examination of exfoliated cells rather than biopsy specimens is more convenient for the screening of patients at high risk of cervical cancer. While a number of independent articles on the usefulness of in situ hybridization (ISH) or P16 immunocytochemistry have been published [25][26][27][28][29][30][31][32][33][34][35][36][37][38][39], there have been no reports of the usefulness of these techniques applied together to LBC specimens, with the results interpreted in relation to confirmed histological findings. We examined the physical status of the HPV DNA by ISH and the level of expression of the P16 protein by immunocytochemistry in 153 LBC specimens, along with the corresponding biopsy specimens that were available for 106 patients.…”

Section: Introductionmentioning

confidence: 99%

Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

et al. 2008

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Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

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Genital Human Papillomavirus Testing by in Situ Hybridization in Liquid Atypical Cytologic Materials and Follow-up Biopsies

Cited by 9 publications

References 5 publications

A Human Papillomavirus Testing System in Women with Abnormal Pap Results

A Human Papillomavirus Testing System in Women with Abnormal Pap Results

Papillomas of the External Ear Canal: Report of Ten cases in Chinese Patients with HPV In Situ Hybridization

Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

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