Genitourinary dysfunction in male rats after bilateral neurectomy of the motor branch of the sacral plexus

Juárez, Margarita; Hernández, Isela; Cruz, Yolanda

doi:10.1002/nau.22242

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…Nevertheless, our results suggest that the Bgm is relevant for both phases of the micturition because its blockade with lidocaine decreased of the ThP and caused episodes of saline dripping. Similar results have been reported in rats in which the transection of the motor branch of the pudendal nerve causes an increased number of small urine marks suggesting urinary incontinence . The voiding phase of micturition is also affected by the blocking of the Bgm activity by lidocaine, reducing the VV, and the MP.…”

Section: Discussionsupporting

confidence: 85%

“…Similar results have been reported in rats in which the transection of the motor branch of the pudendal nerve causes an increased number of small urine marks suggesting urinary incontinence. 24,25 The voiding phase of micturition is also affected by the blocking of the Bgm activity by lidocaine, reducing the VV, and the MP. Both variables are involved in the contractility of detrusor.…”

Section: Discussionmentioning

confidence: 99%

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Anatomic and functional properties of bulboglandularis striated muscle support its contribution as sphincter in female rabbit micturition

Corona-Quintanilla

López-Juárez

Zempoalteca

et al. 2015

Neurourology and Urodynamics

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Anatomic and functional properties of bulboglandularis striated muscle support its contribution as sphincter in female rabbit micturition

Corona-Quintanilla

López-Juárez

Zempoalteca

et al. 2015

Neurourology and Urodynamics

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“…The female rats in this study demonstrated stereotyped behavior of micturition, similar to that described previously in male rats (24,25). The design of the top of the cage with the food and water container in the center of the jumbo cage VD (B) animals.…”

Section: 56)supporting

confidence: 77%

“…Micturition was recorded as previously described (24). The recordings took place in the last 6 h of dark and the first 6 h of light.…”

Section: Methodsmentioning

confidence: 99%

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Neuroanatomic and behavioral correlates of urinary dysfunction induced by vaginal distension in rats

Palacios-Galicia

Juárez

Morán

et al. 2016

American Journal of Physiology-Renal Physiology

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-The aim of the present study was to use a model of simulated human childbirth in rats to determine the damage to genitourinary structures and behavioral signs of urinary dysfunction induced by vaginal distension (VD) in female rats. In experiment 1, the length of the genitourinary tract and the nerves associated with it were measured immediately after simulated human delivery induced by VD or sham (SH) procedures. Electroneurograms of the dorsal nerve of the clitoris (DNC) were also recorded. In experiment 2, histological characteristics of the bladder and major pelvic ganglion of VD and SH rats were evaluated. In experiment 3, urinary parameters were determined in conscious animals during 6 h of dark and 6 h of light before and 3 days after VD or SH procedures. VD significantly increased distal vagina width (P Ͻ 0.001) and the length of the motor branch of the sacral plexus (P Ͻ 0.05), DNC (P Ͻ 0.05), and vesical nerves (P Ͻ 0.01) and decreased DNC frequency and amplitude of firing. VD occluded the pelvic urethra, inducing urinary retention, hematomas in the bladder, and thinness of the epithelial (P Ͻ 0.05) and detrusor (P Ͻ 0.01) layers of the bladder. Major pelvic ganglion parameters were not modified after VD. Rats dripped urine in unusual places to void, without the stereotyped behavior of micturition after VD. The neuroanatomic injuries after VD occur alongside behavioral signs of urinary incontinence as determined by a new behavioral tool for assessing micturition in conscious animals. external urethral sphincter; dorsal nerve of the clitoris; major pelvic ganglion; micturition MICTURITION consists of two phases: storage and urine expulsion. During storage, the detrusor is relaxed while the bladder neck and urethra are activated, preventing involuntary bladder emptying (11). Extrinsic elements such as the levator ani muscle also contribute to the maintenance of continence (3). When the bladder reaches its threshold volume, spinal and supraspinal reflexes are triggered to induce bladder contraction and urethral relaxation, and urine flows through the urethra (11). Damage to the lower urinary tract and/or its innervation can induce urinary dysfunction (3,29,41).Urinary dysfunction affects the health of many women (60). Stress urinary incontinence has been described as involuntary loss of urine during effort and is the most prevalent urinary disorder related to vaginal childbirth, which is known to injure the pudendal nerve and denervate the external urethral sphincter (EUS) (3, 15, 57).Maternal pelvic viscera and nerve damage results from the difficulty of human childbirth due the large fetal head and brain relative to the maternal pelvis size. Neonates at birth have heads that are close to the size of the maternal birth canal through which they must pass during the second stage of parturition (48). Births of fetuses over 4 kg or fetal malposition often prolong parturition (30), retaining the fetus in the pelvic cavity, the main anatomic resistance to fetal expulsion. Prolonged second stage of parturition...

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Striated Perineal Muscles: Location of Somatic and Autonomic Neurons Projecting to the Male Pig Ischiocavernous Muscle. Neurochemical Features of the Sympathetic Subset

Botti¹,

Ragionieri²,

Cacchioli³

et al. 2017

The Anatomical Record

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The location, number and size of the central and peripheral neurons innervating the ischiocavernous muscle (ICM) were studied in male pigs by means of Fast Blue (FB) retrograde neuronal tracing. Moreover the immunohistochemical properties of the sympathetic ganglia were investigated combining the double immunolabeling method. After injection of FB into the left ICM, a mean number of 245.3 ± 134.9 labeled neurons were found in the ipsilateral ventral horn of the S1-S3 segments of the spinal cord (SC), 129.7 ± 45.5 in the L6-S3 ipsilateral and S2-S3 contralateral spinal ganglia (SGs), 2279.3 ± 622.1 in the ipsilateral L2-S2 and contralateral L5-S2 sympathetic trunk ganglia (STGs), 541.7 ± 158 in the bilateral caudal mesenteric ganglia (CMGs), and 78.3 ± 35.8 in the microganglia of the pelvic plexus (PGs). The mean area of the ICM projecting neurons was 1217 ± 69.7 μm in the SC, 2737.5 ± 176.5 μm in the SGs, 982.8 ± 36.8 μm in the STGs, 865.9 ± 39.14 μm in the CMGs and 426.2 ± 24.72 μm in the PGs. The FB positive neurons of autonomic ganglia contained Dopamine β hydroxylase, vesicular acetylcholine transporter, neuronal nitric oxyde sinthase, calcitonine gene related peptide, leu-enkephaline, neuropeptide Y, substance P, vasoactive intestinal polypeptide, and somatostatine often colocalized with tyrosine hydroxylase. The particular localization of the motor somatic nucleus, the abundant autonomic innervation and the qualitatively different content of ICM projecting sympathetic neurons suggest a complex regulation of this striated muscle involved in involuntary functions, such as the erection, ejaculation, micturition and defecation. Anat Rec, 301:837-848, 2018. © 2017 Wiley Periodicals, Inc.

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Genitourinary dysfunction in male rats after bilateral neurectomy of the motor branch of the sacral plexus

Cited by 7 publications

References 24 publications

Anatomic and functional properties of bulboglandularis striated muscle support its contribution as sphincter in female rabbit micturition

Anatomic and functional properties of bulboglandularis striated muscle support its contribution as sphincter in female rabbit micturition

Neuroanatomic and behavioral correlates of urinary dysfunction induced by vaginal distension in rats

Striated Perineal Muscles: Location of Somatic and Autonomic Neurons Projecting to the Male Pig Ischiocavernous Muscle. Neurochemical Features of the Sympathetic Subset

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